Tethering antimicrobial peptides: Current status and potential challenges

Onaizi, Sagheer A.; Leong, Susanna Su Jan

doi:10.1016/j.biotechadv.2010.08.012

Cited by 272 publications

(262 citation statements)

References 90 publications

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“…The MRSA count on Ti-P-EN discs was reduced by 1.64 log units compared to that on Ti discs, demonstrating the clinical potential of such coatings to resist MRSA or other bacterial contamination. The potential mechanism of the prophylactic efficacy of the enoxacin-bonded titanium observed in this study may be divided into two parts, including the antiadhesive property at the early stage, because the bacteria were killed on contact, and the flexible PEG spacers (17), which allowed the enoxacin to react with the bacteria and clear the bacteria adhering to the implant surface.…”

Section: Discussionmentioning

confidence: 99%

Covalent Immobilization of Enoxacin onto Titanium Implant Surfaces for Inhibiting Multiple Bacterial Species Infection and In Vivo Methicillin-Resistant Staphylococcus aureus Infection Prophylaxis

Nie

Long

et al. 2017

Antimicrob Agents Chemother

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Infection is one of the most important causes of titanium implant failure in vivo. A developing prophylactic method involves the immobilization of antibiotics, especially vancomycin, onto the surface of the titanium implant. However, these methods have a limited effect in curbing multiple bacterial infections due to antibiotic specificity. In the current study, enoxacin was covalently bound to an aminefunctionalized Ti surface by use of a polyethylene glycol (PEG) spacer, and the bactericidal effectiveness was investigated in vitro and in vivo. The titanium surface was amine functionalized with 3-aminopropyltriethoxysilane (APTES), through which PEG spacer molecules were covalently immobilized onto the titanium, and then the enoxacin was covalently bound to the PEG, which was confirmed by X-ray photoelectron spectrometry (XPS). A spread plate assay, confocal laser scanning microscopy (CLSM), and scanning electron microscopy (SEM) were used to characterize the antimicrobial activity. For the in vivo study, Ti implants were inoculated with methicillin-resistant Staphylococcus aureus (MRSA) and implanted into the femoral medullary cavity of rats. The degree of infection was assessed by radiography, micro-computed tomography, and determination of the counts of adherent bacteria 3 weeks after surgery. Our data demonstrate that the enoxacin-modified PEGylated Ti surface effectively prevented bacterial colonization without compromising cell viability, adhesion, or proliferation in vitro. Furthermore, it prevented MRSA infection of the Ti implants in vivo. Taken together, our results demonstrate that the use of enoxacin-modified Ti is a potential approach to the alleviation of infections of Ti implants by multiple bacterial species.

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Section: Discussionmentioning

confidence: 99%

Covalent Immobilization of Enoxacin onto Titanium Implant Surfaces for Inhibiting Multiple Bacterial Species Infection and In Vivo Methicillin-Resistant Staphylococcus aureus Infection Prophylaxis

Nie

Long

et al. 2017

Antimicrob Agents Chemother

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“…In this regard, the design and development of methodologies that can specifically focus the action of antimicrobial agents on the polymeric surface is very attractive. Based on this issue, antimicrobial peptides (AMPs) are an attractive alternative to classical antimicrobial drugs and antibiotics, due to their broad spectrum activity, relatively nontoxic nature towards host cells and are effective at low concentrations [83][84][85]. These natural oligopeptides, which are components of the immune natural defense system of various living organisms, exert their activity by creating pores or structural defects in the bacteria cell membranes.…”

Section: Antimicrobial and Antibacterial Coatingsmentioning

confidence: 99%

Polymer Brushes with Precise Architectures for Molecular Biorecognition

Pérez-Perrino

Molina

Navarro

2015

Design of Polymeric Platforms for Selective Biorecognition

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“…The hospital infections and various dermatomes are among the potential health risks [1]. Accordingly, the demand for antimicrobial floor and wall coating materials in clinical, industrial and domestic spaces, especially on wettable surfaces as well as antimicrobial textiles is expected to increase.…”

Section: Introductionmentioning

confidence: 99%

Formation of Antibacterial Effect on Ceramic Tile Surfaces

Özcan¹,

Açıkbaş²,

Açıkbaş³

2017

ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY a - Applied Sciences and Engineering

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Biocidal antimicrobial molecular barrier (BAMB) solutions are known to provide antimicrobial effect on the surfaces in industrial applications. However, there has been a lack of scientific reports about the subject in the literature. In this study, in order to impart an antimicrobial surface property on ceramic surfaces, a BAMB solution was applied on gloss fired ceramic wall tile substrates and the surface antimicrobial activity results were compared with that of plain wall tiles (without BAMB application). The ceramic surfaces were cleaned, and stove dried at120°C prior to spray coating with a BAMB solution. The coated substrates were dried in the ambient. The intactness of the coatings was checked with the bromophenol blue test. The microstructural and molecular characterization of the BAMB coated surfaces were carried out with SEM imaging and surface FTIR, respectively. The antimicrobial activity tests of the surfaces were conducted according to ASTM E2180-07 (Standard Test Method for Determining the Activity of Incorporated Antimicrobial Agent in Polymeric or Hydrophobic Materials). The microorganisms used were Staphylococcus aureus (ATCC 6538) and Pseudomonas aeruginosa (ATCC 15442) bacteria. The BAMB coated surfaces showed less flocculent bacterial growth in comparison to uncoated ceramic surfaces leading to the conclusion that the BAMB improved the antimicrobial property.

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Tethering antimicrobial peptides: Current status and potential challenges

Cited by 272 publications

References 90 publications

Covalent Immobilization of Enoxacin onto Titanium Implant Surfaces for Inhibiting Multiple Bacterial Species Infection and In Vivo Methicillin-Resistant Staphylococcus aureus Infection Prophylaxis

Covalent Immobilization of Enoxacin onto Titanium Implant Surfaces for Inhibiting Multiple Bacterial Species Infection and In Vivo Methicillin-Resistant Staphylococcus aureus Infection Prophylaxis

Polymer Brushes with Precise Architectures for Molecular Biorecognition

Formation of Antibacterial Effect on Ceramic Tile Surfaces

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