Terrestrial and marine Antarctic fungi extracts active against <i>Xanthomonas citri</i>
 subsp. <i>citri</i>

Vieira, Gabrielle; Puric, Jelena; Morão, Luana Galvão; Santos, Juliana Aparecida dos; Inforsato, Fábio José; Sette, Lara Durães; Ferreira, Henrique; Sass, Daiane Cristina

doi:10.1111/lam.12890

Cited by 31 publications

(18 citation statements)

References 21 publications

(33 reference statements)

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“…With the exception of Plectosphaerella, all the other genera were also identified in a similar study, which showed the diversity in sea foam-derived fungi on the coast of the Canadian Prince Edward Island [4]. The literature shows that these species are common across a wide array of niches in both terrestrial and marine environments [43][44][45][46]. Similar to previous studies, the majority of sea foam fungal isolates found in this study are ubiquitous saprophytes [1,4].…”

Section: Discussionsupporting

confidence: 87%

Mining the Metabolome and the Agricultural and Pharmaceutical Potential of Sea Foam-Derived Fungi

et al. 2020

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Sea foam harbors a diverse range of fungal spores with biological and ecological relevance in marine environments. Fungi are known as the producers of secondary metabolites that are used in health and agricultural sectors, however the potentials of sea foam-derived fungi have remained unexplored. In this study, organic extracts of six foam-derived fungal isolates belonging to the genera Penicillium, Cladosporium, Emericellopsis and Plectosphaerella were investigated for their antimicrobial activity against plant and human pathogens and anticancer activity. In parallel, an untargeted metabolomics study using UPLC-QToF–MS/MS-based molecular networking (MN) was performed to unlock their chemical inventory. Penicillium strains were identified as the most prolific producers of compounds with an average of 165 parent ions per strain. In total, 49 known mycotoxins and functional metabolites were annotated to specific and ubiquitous parent ions, revealing considerable chemical diversity. This allowed the identification of putative new derivatives, such as a new analog of the antimicrobial tetrapeptide, fungisporin. Regarding bioactivity, the Penicillium sp. isolate 31.68F1B showed a strong and broad-spectrum activity against seven plant and human pathogens, with the phytopathogen Magnaporthe oryzae and the human pathogen Candida albicans being the most susceptible (IC50 values 2.2 and 6.3 µg/mL, respectively). This is the first study mining the metabolome of the sea foam-derived fungi by MS/MS-based molecular networking, and assessing their biological activities against phytopathogens.

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Section: Discussionsupporting

confidence: 87%

Mining the Metabolome and the Agricultural and Pharmaceutical Potential of Sea Foam-Derived Fungi

et al. 2020

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“…Other studies reported the bioactivity of this genus against Xanthomonas (Henríquez et al, 2014), including some from our research group, against X. citri, X. euvesicatoria and X. axonopodis pv. passiflorae (Vieira et al, 2018, but so far no molecules have been described.…”

Section: Discussionmentioning

confidence: 99%

“…New microorganisms and natural products can be isolated from extreme environments, such as Antarctica (Pikuta et al, 2007). Many studies highlight the potential of the Antarctic environment in the production of bioactive metabolites (Furbino et al, 2014), antifungal substances (Svahn et al, 2015) and active compounds against Xanthomonas species, including X. citri (Moncheva et al, 2002;Encheva-Malinova et al, 2015;Puric et al, 2018;Vieira et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Anti-Xanthomonas activity of Antarctic fungi crude extracts

Ferrarezi¹,

Santos²,

Sette³

et al. 2019

Afr. J. Biotechnol.

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Agriculture suffers considerable losses in its production caused by plant diseases. In the citrus culture some problems were associated with citrus canker caused by Xanthomonas citri subsp. citri. That same way, sugarcane plantations are impaired to leaf scald disease caused by Xanthomonas albilineans. One of the measures used to contain citrus canker is the spray of cupric bactericides; however for scalding leaves there are no satisfactory control. Due to the phytosanitary problem caused by Xanthomonas (X. citri and X. albilineans) and by the difficulty of its control, the search for new forms of defense, less harmful to the environment, has become increasingly required. Thus, the aim of this work was to obtain crude organic extracts from filamentous fungi isolated from Antarctic soil samples and assess its bioactivity potential against X. citri and X. albilineans. One hundred and twenty-two extracts were tested, seven extracts inhibited the cell growth of X. citri, one was bioactive only against X. albilineans and one extract inhibited the cell growth of both bacteria. The bioactive extracts had a mean inhibition value of 96% against both bacteria. The values of MIC90 and MBC of bioactive extracts were also determined; for X. citri the isolate 3.1 Fe presented the lowest values of MIC90 (0.28 mg/mL) and MBC (1.0 mg/mL) and the two bioactive isolates for X. albilineans (1.1-Fe and B-Fe) presented the same values of MIC90 (1.4 mg/mL) and MBC (1.5 mg/mL). The filamentous fungi that produced positive extracts were identified as belonging to the genus Pseudogymnoascus (n=8) and Cladosporium (n=1). The filamentous fungi isolated from Antarctic soil produced compounds with bioactivity against phytopathogens from the Xanthomonas genus.

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“…To date, the species P. appendiculatus , P. destructans , P. pannorum , P. verrucosus , and P. vinaceous have been isolated from different Antarctic environments (Loque et al, 2010; Gomes et al, 2018; Alves et al, 2019; Kochkina et al, 2019; Rosa et al, 2019). Several studies have shown that extracts of these fungi produce bioactive metabolites with potential application, including antibacterial, antifungal, tripanocidal, herbicidal, and antitumoral activities (Furbino et al, 2014; Henríquez et al, 2014; Gonçalves et al, 2015; Gomes et al, 2018; Purić et al, 2018; Vieira et al, 2018). On the other hand, Pseudogymnoascus strains of Antarctic origin also have shown ability to produce cold-adapted enzymes with potential application in several industrial processes (Loperena et al, 2012; Poveda et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Genetic Transformation of the Filamentous Fungus Pseudogymnoascus verrucosus of Antarctic Origin

et al. 2019

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Cold-adapted fungi isolated from Antarctica, in particular those belonging to the genus Pseudogymnoascus, are producers of secondary metabolites with interesting bioactive properties as well as enzymes with potential biotechnological applications. However, at genetic level, the study of these fungi has been hindered by the lack of suitable genetic tools such as transformation systems. In fungi, the availability of transformation systems is a key to address the functional analysis of genes related with the production of a particular metabolite or enzyme. To the best of our knowledge, the transformation of Pseudogymnoascus strains of Antarctic origin has not been achieved yet. In this work, we describe for the first time the successful transformation of a Pseudogymnoascus verrucosus strain of Antarctic origin, using two methodologies: the polyethylene glycol (PEG)-mediated transformation, and the electroporation of germinated conidia. We achieved transformation efficiencies of 15.87 ± 5.16 transformants per μg of DNA and 2.67 ± 1.15 transformants per μg of DNA for PEG-mediated transformation and electroporation of germinated conidia, respectively. These results indicate that PEG-mediated transformation is a very efficient method for the transformation of this Antarctic fungus. The genetic transformation of Pseudogymnoascus verrucosus described in this work represents the first example of transformation of a filamentous fungus of Antarctic origin.

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Terrestrial and marine Antarctic fungi extracts active against Xanthomonas citri subsp. citri

Cited by 31 publications

References 21 publications

Mining the Metabolome and the Agricultural and Pharmaceutical Potential of Sea Foam-Derived Fungi

Mining the Metabolome and the Agricultural and Pharmaceutical Potential of Sea Foam-Derived Fungi

Anti-Xanthomonas activity of Antarctic fungi crude extracts

Genetic Transformation of the Filamentous Fungus Pseudogymnoascus verrucosus of Antarctic Origin

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