Term Stillbirth Caused by Oral Fusobacterium nucleatum

Han, Yiping W.; Fardini, Yann; Chen, Casey; Iacampo, Karla G.; Peraino, Victoria A.; Shamonki, Jaime; Redline, Raymond W.

doi:10.1097/aog.0b013e3181cb9955

Cited by 250 publications

(251 citation statements)

References 8 publications

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“…These studies demonstrated that bacteria administered intravenously make their way to placental tissue, proliferate there, and then invade into amniotic fluid and fetal tissues, eventually resulting in fetal resorption and stillbirth (Han et al 2004;Liu et al 2007). Further evidence for the hematogenous route of dissemination from oral to uterine tissue has come from analysis of bacterial isolates from oral and amniotic fluid sources in the same individual (Han et al 2010). Another study developed primer sets capable of distinguishing between the five subspecies of F. nucleatum and performed PCR using these primer sets on DNA isolated from different body sites of 10 women with localized periodontal pockets who delivered preterm (Gonzales-Marin et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…A less appreciated site of F. nucleatum infection is the reproductive tract; the organism can cause both vaginal (Hillier et al 1993;Hitti et al 2001;Holst et al 1994) and intrauterine infections (Altshuler and Hyde 1985;Chaim and Mazor 1992;Digiulio et al 2010). In fact, F. nucleatum is one of the most frequently isolated organisms from amniotic fluid of pregnant women who experience preterm labor (Chaim and Mazor 1992;Hill 1998;Bearfield et al 2002;Hitti et al 2001), and has also been associated with spontaneous miscarriage and stillbirth (Altshuler and Hyde 1985;Chaim and Mazor 1992;Han et al 2010). Periodontal disease, which is thought to affect~40% of reproductive age women (Boggess 2008), has also been linked with increased risks of pregnancy complications such as preterm birth and low birth weight (Offenbacher et al 1996;Dasanayake 1998;Pitiphat et al 2008;Guimaraes et al 2010;Saddki et al 2008).…”

Section: Introductionmentioning

confidence: 99%

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Discovery and characterization ofde novosialic acid biosynthesis in the phylumFusobacterium

Lewis¹,

Robinson²,

Agarwal³

et al. 2016

Glycobiology

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Sialic acids are nine-carbon backbone carbohydrates found in prominent outermost positions of glycosylated molecules in mammals. Mimicry of sialic acid (N-acetylneuraminic acid, Neu5Ac) enables some pathogenic bacteria to evade host defenses. Fusobacterium nucleatum is a ubiquitous oral bacterium also linked with invasive infections throughout the body. We employed multidisciplinary approaches to test predictions that F. nucleatum engages in de novo synthesis of sialic acids. Here we show that F. nucleatum sbsp. polymorphum ATCC10953 NeuB (putative Neu5Ac synthase) restores Neu5Ac synthesis to an Escherichia coli neuB mutant. Moreover, purified F. nucleatum NeuB participated in synthesis of Neu5Ac from N-acetylmannosamine and phosphoenolpyruvate in vitro. Further studies support the interpretation that F. nucleatum ATCC10953 NeuA encodes a functional CMP-sialic acid synthetase and suggest that it may also contain a C-terminal sialic acid O-acetylesterase. We also performed BLAST queries of F. nucleatum genomes, revealing that only 4/31 strains encode a complete pathway for de novo Neu5Ac synthesis. Biochemical studies including mass spectrometry were consistent with the bioinformatic predictions, showing that F. nucleatum ATCC10953 synthesizes high levels of Neu5Ac, whereas ATCC23726 and ATCC25586 do not express detectable levels above background. While there are a number of examples of sialic acid mimicry in other phyla, these experiments provide the first biochemical and genetic evidence that a member of the phylum Fusobacterium can engage in de novo Neu5Ac synthesis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Discovery and characterization ofde novosialic acid biosynthesis in the phylumFusobacterium

Lewis¹,

Robinson²,

Agarwal³

et al. 2016

Glycobiology

View full text Add to dashboard Cite

show abstract

“…A case report of a stillbirth caused by F. nucleatum from the mother's mouth highlights the fact that an oral periodontal pathogen can, by hematologic pathway, colonize placenta and provoke fetal complications [20].…”

Section: Association Of Periodontal Health To Complications During Prmentioning

confidence: 99%

Association of Periodontal Disease and Pre-term Low Birth Weight Infants

Varadan

Ramamurthy

2014

J Obstet Gynecol India

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“…Hill demonstrated that Fusobacterium nucleatum can be isolated from amniotic fluid cultures of women with preterm labour and intact membranes suggesting the transient bacteraemia had originated from the mouth via haematogenous spread and infection of the amniotic fluid through the placenta [28]. More recently, Han and colleagues have reported a term stillbirth case by association was caused by Fusobacterium nucleatum, she had previously demonstrated that Fusobacterium nucleatum induced premature and term stillbirths in pregnant mice [29,30].…”

Section: Discussionmentioning

confidence: 99%

Can Oral Anaerobic Bacteria cause Adverse Pregnancy Outcomes?/ Дали Оралните Анаеробни Бактерии Можат Да Доведат До Лоши Опстетрички Исходи?

Andonova

Iliev

Živković

et al. 2015

Prilozi

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Aim: Maternal periodontal infection has been recognized as a risk factor for premature and low birthweight infants. It is suspected that pathogens causing periodontal disease may translocate to the amniotic cavity and so contribute to triggering an adverse pregnancy outcome. The aim of this study was to evaluate whether the presence of specific periodontal pathogens may influence the incidence of preterm labor and premature birth. Material and Methods: This study was designed as a hospital-based case-control study. A total of 70 pregnant women, aged 18-40 with single live pregnancy were recruited from the Departement of Gynecolgy and Obstetrics at a General hospital in Sibenik, Croatia, between March 2013 to March 2014. The case group: 30 pregnant women who were hospitalised with signs of premature labor. Control group: 40 patients with normal pregnancy post-delivery up to 48 hrs, who had given birth at term, and the baby had a weight of more than 2500 gr. These women had undergone microbiological examination at the time of recruitment, microbial samples, paper point subgingival swabs were obtained in both groups and processed by anaerobic culturing. Standard procedures were used for culture and identification of bacteria. Information was collected on demographics, health behaviors, and obstetric and systemic diseases that may have influence the premature delivery. Results: The levels of periodontal pathogens tended to be higher in the premature (case group) labor compared to the term deliveries (control group). Levels of Porphyromonas gingivalis, Fuscobacterium nucleatum, Actinomyces actinomycetecomitans were statistically significantly higher in premature births as compared to term deliveries, adjusting for baseline levels. The joint effects of red and orange microbial clusters were significantly higher in the premature group compared to the term group. Conclusions: The study shows a significant association betwen periodontal anaerobic infection and adverse pregnancy outcome. High levels of periodontal pathogens during pregnancy are associated with an increased risk for preterm delivery. Further studies elucidating the role of the microbial load and maternal immune response as related to pregnancy outcome seem merited.

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Term Stillbirth Caused by Oral Fusobacterium nucleatum

Abstract: Background-Intrauterine infection is a recognized cause of adverse pregnancy outcome but the source of infection is often undetermined. We report a case of stillbirth caused by Fusobacterium nucleatum that originated in the mother's mouth.

Cited by 250 publications

References 8 publications

Discovery and characterization ofde novosialic acid biosynthesis in the phylumFusobacterium

Discovery and characterization ofde novosialic acid biosynthesis in the phylumFusobacterium

Association of Periodontal Disease and Pre-term Low Birth Weight Infants

Can Oral Anaerobic Bacteria cause Adverse Pregnancy Outcomes?/ Дали Оралните Анаеробни Бактерии Можат Да Доведат До Лоши Опстетрички Исходи?

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