Teratology and Carbonic Anhydrase Inhibition

The Effect of Acetazolamide on Cerebral Blood Flow and Oxygen Utilization in the Rhesus Monkey

“…This effect occurs within minutes of acetazolamide administration, which is consistent with the 3 min in vitro equilibration time between erythrocytic carbonic anhydrase and acetazolamide reported by Maren et al. (18). Furthermore, these data suggest that the effect may, in fact, be primarily achieved through an effect of acetazolamide on the erythrocytic carbonic anhydrase, because it is unlikely that a significant amount ofthe drug can cross the bloodbrain barrier in this time.…”

The Effect of Acetazolamide on Cerebral Blood Flow and Oxygen Utilization in the Rhesus Monkey

“…This effect occurs within minutes of acetazolamide administration, which is consistent with the 3 min in vitro equilibration time between erythrocytic carbonic anhydrase and acetazolamide reported by Maren et al. (18). Furthermore, these data suggest that the effect may, in fact, be primarily achieved through an effect of acetazolamide on the erythrocytic carbonic anhydrase, because it is unlikely that a significant amount ofthe drug can cross the bloodbrain barrier in this time.…”

Carbonic anhydrase in the sarcoplasmic reticulum of rabbit skeletal muscle.

“…The slope of the regression line represents the inhibition constant, KI, of the enzyme for acetazolamide if the inhibition is non-competitive. Such a type of inhibition has been reported for carbonic anhydrase II under similar assay conditions by various authors (Maren, Parcell & Malik, 1960;Leibman & Alford, 1960;Maren & Wiley, 1968).…”

Inhibition of carbonic anhydrase by parathyroid hormone and cyclic AMP in rat renal cortex in vitro.

“…This value is higher than the measured cyclic AMP concentration, 0.05 mM, in the isolated renal tubular cells (33), and the measured value, 26.2 nM, in the mixture of carbonic anhydrase after 10 min incubation with PTH in the present experiments. Here again, it should be emphasized that the optimal concentration of cyclic AMP in an in vitro system is not directly comparable to that in vivo.…”

The Effect of Acetazolamide on Cerebral Blood Flow and Oxygen Utilization in the Rhesus Monkey

“…This effect occurs within minutes of acetazolamide administration, which is consistent with the 3 min in vitro equilibration time between erythrocytic carbonic anhydrase and acetazolamide reported by Maren et al. (18). Furthermore, these data suggest that the effect may, in fact, be primarily achieved through an effect of acetazolamide on the erythrocytic carbonic anhydrase, because it is unlikely that a significant amount ofthe drug can cross the bloodbrain barrier in this time.…”

Carbonic anhydrase in the sarcoplasmic reticulum of rabbit skeletal muscle.

“…The slope of the regression line represents the inhibition constant, KI, of the enzyme for acetazolamide if the inhibition is non-competitive. Such a type of inhibition has been reported for carbonic anhydrase II under similar assay conditions by various authors (Maren, Parcell & Malik, 1960;Leibman & Alford, 1960;Maren & Wiley, 1968).…”

Inhibition of carbonic anhydrase by parathyroid hormone and cyclic AMP in rat renal cortex in vitro.

“…This value is higher than the measured cyclic AMP concentration, 0.05 mM, in the isolated renal tubular cells (33), and the measured value, 26.2 nM, in the mixture of carbonic anhydrase after 10 min incubation with PTH in the present experiments. Here again, it should be emphasized that the optimal concentration of cyclic AMP in an in vitro system is not directly comparable to that in vivo.…”

The Effect of Acetazolamide on Cerebral Blood Flow and Oxygen Utilization in the Rhesus Monkey

“…This effect occurs within minutes of acetazolamide administration, which is consistent with the 3 min in vitro equilibration time between erythrocytic carbonic anhydrase and acetazolamide reported by Maren et al. (18). Furthermore, these data suggest that the effect may, in fact, be primarily achieved through an effect of acetazolamide on the erythrocytic carbonic anhydrase, because it is unlikely that a significant amount ofthe drug can cross the bloodbrain barrier in this time.…”

Carbonic anhydrase in the sarcoplasmic reticulum of rabbit skeletal muscle.

“…The slope of the regression line represents the inhibition constant, KI, of the enzyme for acetazolamide if the inhibition is non-competitive. Such a type of inhibition has been reported for carbonic anhydrase II under similar assay conditions by various authors (Maren, Parcell & Malik, 1960;Leibman & Alford, 1960;Maren & Wiley, 1968).…”

Inhibition of carbonic anhydrase by parathyroid hormone and cyclic AMP in rat renal cortex in vitro.

“…This value is higher than the measured cyclic AMP concentration, 0.05 mM, in the isolated renal tubular cells (33), and the measured value, 26.2 nM, in the mixture of carbonic anhydrase after 10 min incubation with PTH in the present experiments. Here again, it should be emphasized that the optimal concentration of cyclic AMP in an in vitro system is not directly comparable to that in vivo.…”