Temporal variation in pools of amino acids, inorganic and microbial N in a temperate grassland soil

Warren, Charles R.; Taranto, Maria T.

doi:10.1016/j.soilbio.2009.11.017

Cited by 48 publications

(19 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…woodland in the Snowy Mountains of Australia (36°06′S; 148°32′E; 1500 m altitude). The soil is classified as a humic umbrosol (World Reference Base) or chernic tenosol (Isbell, 2002) and has been described previously (Warren, 2009b; Warren & Taranto, 2010). The soil is a well‐drained sandy loam without coarse fragments > 2 mm, pH (H 2 O) is 4.5, organic C (Walkley and Black method) is 12–17%, and total N is 0.2–0.3%.…”

Section: Methodsmentioning

confidence: 99%

Post‐uptake metabolism affects quantification of amino acid uptake

Warren

2011

New Phytologist

Self Cite

View full text Add to dashboard Cite

Summary• The quantitative significance of amino acids to plant nutrition remains controversial. This experiment determined whether post-uptake metabolism and root to shoot export differ between glycine and glutamine, and examined implications for estimation of amino acid uptake.• Field soil containing a Eucalyptus pauciflora seedling was injected with uniformly 13 C-and 15 N-labelled glycine or glutamine. I quantified 15 N and 13 C excess in leaves and roots and intact labelled amino acids in leaves, roots and stem xylem sap. A tunable diode laser quantified fluxes of 12 CO 2 and 13 CO 2 from leaves and soil.• 60-360 min after addition of amino acid, intact molecules of U-13 C, 15 N glutamine were < 5% of 15 N excess in roots, whereas U-13 C, 15 N glycine was 30-100% of 15 N excess in roots. Intact molecules of glutamine, but not glycine, were exported from roots to shoots.• Post-uptake metabolism and transport complicate interpretation of isotope labelling such that root and shoot contents of intact amino acid, 13 C and 15 N may not reflect rates of uptake. Future experiments should focus on reconciling discrepancies between intact amino acid, 13 C and 15 N by determining the turnover of amino acids within roots. Alternatively, post-uptake metabolism and transport could be minimized by harvesting plants within minutes of isotope addition.

show abstract

Section: Methodsmentioning

confidence: 99%

Post‐uptake metabolism affects quantification of amino acid uptake

Warren

2011

New Phytologist

Self Cite

View full text Add to dashboard Cite

show abstract

“…Additional complications are that the profile and amount of metabolites extracted are strongly dependent on the type of extractant (e.g. water versus 1 M KCl, Warren and Taranto 2010), shaking time and ratio of soil to extractant (Jones and Willett 2006). The limitations of soil extracts mean that they are unlikely to be representative of the in situ milieu, but yield data that can be compared within studies and (with rigorous control of extraction conditions) among studies.…”

Section: Sampling Of Organic N From Soil Solutionmentioning

confidence: 99%

Organic N molecules in the soil solution: what is known, what is unknown and the path forwards

Warren

2013

Plant Soil

Self Cite

View full text Add to dashboard Cite

Background Many biological questions about N availability and the N cycle require knowledge of the abundance and identity of molecules comprising the pool of organic N. Moreover, basic knowledge of the molecular composition of the soil solution can give rise to new hypotheses via data-driven or inductive reasoning. Scope This paper examines the composition of organic N molecules in the soil solution. Our perception of organic N in the soil solution is shaped by analytical approaches, and thus I briefly review approaches for sampling and analysis of the soil solution. I give examples of hypotheses generated by knowledge of the molecular composition of organic N and conclude by suggesting priorities for future research. Conclusions Studies of the molecular composition of organic N are very much in their infancy. Amino acids, their oligomers and polymers are consistently large components of the pool of organic N. The soil solution also contains organic N compounds from at least another 12 compound classes, but almost nothing is known about their functional significance. Uncovering the role of these other compounds in the N cycle would enrich our understanding of organic N and the N cycle, and place studies of amino acids and their polymers in a broader context.

show abstract

“…Determining the rewetting dynamics of N compounds has been challenging because it is difficult to monitor minute-to hour-scale changes in N supply in intact soil. Studies have mostly relied on destructive sampling, but disturbances during soil collection and analysis can alter microbial processes (Dumont et al, 2006;Lee et al, 2007) and N concentrations (Rousk and Jones, 2010;Warren and Taranto, 2010;Inselsbacher, 2014). For instance, destructive sampling may overestimate N availability because bulk soil extractions may release protected N in organo-mineral complexes that had not been available for microbial uptake (Van Gestel et al, 1991;Fierer and Schimel, 2003).…”

Section: Introductionmentioning

confidence: 99%

Linking NO and N2O emission pulses with the mobilization of mineral and organic N upon rewetting dry soils

Leitner

Homyak

Blankinship

et al. 2017

Soil Biology and Biochemistry

View full text Add to dashboard Cite

Drying and rewetting of soils triggers a cascade of physical, chemical, and biological processes; understanding these responses to varying moisture levels becomes increasingly important in the context of changing precipitation patterns. When soils dry and water content decreases, diffusion is limited and substrates can accumulate. Upon rewetting, these substrates are mobilized and can energize hot moments of intense biogeochemical cycling, leading to pulses of trace gas emissions. Until recently, it was difficult to follow the rewetting dynamics of nutrient cycling in the field without physically disturbing the soil. Here

show abstract

Temporal variation in pools of amino acids, inorganic and microbial N in a temperate grassland soil

Cited by 48 publications

References 36 publications

Post‐uptake metabolism affects quantification of amino acid uptake

Post‐uptake metabolism affects quantification of amino acid uptake

Organic N molecules in the soil solution: what is known, what is unknown and the path forwards

Linking NO and N2O emission pulses with the mobilization of mineral and organic N upon rewetting dry soils

Contact Info

Product

Resources

About