Temporal Expression Profiles Reveal Potential Targets during Postembryonic Development of Forensically Important Sarcophaga peregrina (Diptera: Sarcophagidae)

Abstract

Sarcophaga peregrina (Robineau-Desvoidy, 1830) is a species of medical and forensic importance. In order to investigate the molecular mechanism during postembryonic development and identify specific genes that may serve as potential targets, transcriptome analysis was used to investigate its gene expression dynamics from the larval to pupal stages, based on our previous de novo-assembled genome of S. peregrina. Totals of 2457, 3656, 3764, and 2554 differentially expressed genes were identified. The specific genes encoding the structural constituent of cuticle were significantly differentially expressed, suggesting that degradation and synthesis of cuticle-related proteins might actively occur during metamorphosis. Molting (20-hydroxyecdysone, 20E) and juvenile (JH) hormone pathways were significantly enriched, and gene expression levels changed in a dynamic pattern during the developmental stages. In addition, the genes in the oxidative phosphorylation pathway were significantly expressed at a high level during the larval stage, and down-regulated from the wandering to pupal stages. Weighted gene co-expression correlation network analysis (WGCNA) further demonstrated the potential regulation mechanism of tyrosine metabolism in the process of puparium tanning. Moreover, 10 consistently up-regulated genes were further validated by qRT-PCR. The utility of the models was then examined in a blind study, indicating the ability to predict larval development. The developmental, stage-specific gene profiles suggest novel molecular markers for age prediction of forensically important flies.

Shotgun metagenomics reveals the gut microbial diversity and functions in Vespa mandarinia (Hymenoptera: Vespidae) at multiple life stages

“…In larvae, oxidative phosphorylation is the most abundant function, followed by nicotinate and nicotinamide metabolism, purine metabolism, and pyrimidine metabolism. The oxidative phosphorylation genes were expressed at a high level during the larval stage of V. mandarinia , which has been observed in other insects (Ren et al, 2022 ). Sufficient ATP was produced through oxidative phosphorylation for energy supply to promote the rapid growth of insect larvae (Bretscher and O'Connor, 2020 ).…”

Genome-wide analysis of long noncoding RNAs and their association in regulating the metamorphosis of the Sarcophaga peregrina (Diptera: Sarcophagidae)

“…Sarcophaga peregrina (Robineau-Desvoidy, 1830) (Diptera: Sarcophagidae), is an important hygiene pest, and as vectors of parasitic disease agents, they can cause myiasis in humans and other mammals [19]. Several human myiasis cases caused by S. peregrina have occurred in different sites, such as the nose, mouth and the eyes, suggesting that proper control measures for flies involved in myiasis in humans are necessary [20].…”

Molecular identification and functional analysis of chitinase genes reveal their importance in the metamorphosis of Sarcophaga peregrina (Diptera: Sarcophagidae)

“…We identified several genes that displayed significant differential expression patterns based on transcriptome datasets obtained from developmental stage comparisons during S. peregrina pupariation ( Supplementary Fig. S1 and Supplementary Table S1 ) ( Ren et al 2022 ). Among them, SpIDGF2 (gene ID: Contig1.824) and SpCht10 (gene ID: Contig11.188) demonstrated a temporal decrease in expression trend during the metamorphosis, suggesting their potential roles ( Supplementary Fig.…”