Temporal expression of the major lignin peroxidase genes of Phanerochaete chrysosporium

Boominathan, K.; D’Souza, Trevor M.; Naidu, P S; Dosoretz, Carlos G.; Reddy, C. A.

doi:10.1128/aem.59.11.3946-3950.1993

Cited by 23 publications

(15 citation statements)

References 18 publications

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“…However, we have shown that PCR of cDNA preparations can be used for more sensitive analysis that also allows the resolution of genes coding for isozymes (4). This is important since it has been shown that there are gene families with differential gene expression (2,4,9,21,33,34,43,44).…”

Section: Discussionmentioning

confidence: 99%

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PCR-mediated analysis of lignocellulolytic gene transcription by Phanerochaete chrysosporium: substrate-dependent differential expression within gene families

Broda

Birch

Brooks

et al. 1995

Appl Environ Microbiol

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We compare the kinetics of appearance of supernatant enzyme activities (lignin peroxidase, manganese peroxidase, and cellulase) and gene expression (LIG, mnp, and cbhI gene families and the unique cbhII gene) in Phanerochaete chrysosporium ME446 when grown on four different carbon sources: ball-milled straw, representing the natural substrate lignocellulose; Avicel as a crystalline cellulose; and high and low concentrations of glucose, in all cases with limiting nitrogen. PCR-based technology utilizing pairs of primers specific for particular genes showed that there is differential expression between and within the families. There were a number of instances of mRNA species being present only on a single day, implying tight regulation of lignocellulose degradation at the mRNA level. The patterns of extracellular enzyme activities and mnp and cbh gene expression are similar whereas LIG gene expression can be detected when no corresponding enzyme activity is observed in the extracellular supernatant. The enzyme produced under these conditions is presumably sequestered by the mycelium and is likely to be functionally significant. Another striking result is that cellulose, in the form of Avicel, elicits the expression of three LIG genes for which there is no expression under the same conditions with the other carbon sources.

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Section: Discussionmentioning

confidence: 99%

“…It has been shown that for both LiPs and MnPs there are families of genes which are differentially expressed (2,4,33,35,43). Cellobiohydrolases have been characterized and show synergy (46), and whereas a complex family of CBHI-encoding genes showing differential expression exists (8,9,41), only a single CBHII-encoding gene exists (44).…”

mentioning

confidence: 99%

PCR-mediated analysis of lignocellulolytic gene transcription by Phanerochaete chrysosporium: substrate-dependent differential expression within gene families

Broda

Birch

Brooks

et al. 1995

Appl Environ Microbiol

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“…Information regarding enzyme expression in white-rot fungi has mostly been obtained from cultures grown in chemically defined liquid media; extensive data concerning LiP isozyme profiles have been gathered from liquid cultures at the level both of protein (5,8,12,30) and of mRNA transcripts (4,5,38,49). Very little data exist for white-rot fungi grown on solid substrates.…”

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confidence: 99%

Expression of lip genes during growth in soil and oxidation of anthracene by Phanerochaete chrysosporium

Bogan

Schoenike

Lamar

et al. 1996

Appl Environ Microbiol

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mRNA extraction from soil and quantitation by competitive reverse transcription-PCR were combined to study the expression of the 10 known lignin peroxidase (lip) genes in anthracene-transforming soil cultures of Phanerochaete chrysosporium. Levels of extractable lipA transcript and protein (LiP H8) were well correlated, although they were separated by a 2-day lag period. The patterns of transcript abundance over time in soil-grown P. chrysosporium varied among the nine lip mRNAs detected; comparison with lip gene expression under different liquid culture conditions suggested an early phase of carbon limitation for the cultures as a whole, which was followed by a transition to nitrogen starvation. Anthracene transformation occurred throughout the 25-day course of the experiment and, therefore, likely involves mechanisms distinct from those involved in oxidation of non-LiP substrate polycyclic aromatic hydrocarbons.

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“…However, this is not the case; in contrast to the LiP H8-encoding genes discussed above, those for H2 can be strongly expressed in N sufficiency (Holzbaur and Tien, 1988;Stewart et al, 1992;Dosoretz et al, 1993). lig5, encoding an H2-like isoenzyme in strain ME446, is expressed when both N and C are limited (James et al, 1992;Brooks et al, 1993;Broda et al, 1995), and CLG4, the equivalent gene in strain BKM-F-1767, is expressed when only N is limited (Boominathan et al, 1993). Together, these studies show that among the lip genes those encoding the H2 proteins are expressed under the widest variety of conditions and ages of culture and from the earliest time points.…”

Section: Lignocellulolytic Gene Expression: Peroxidasesmentioning

confidence: 91%

Lignocellulose degradation by Phanerochaete chrysosporium: gene families and gene expression for a complex process

Broda

Birch

Brooks

et al. 1996

Molecular Microbiology

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Phanerochaete chrysosporium completely degrades lignocellulose. The most recalcitrant component, lignin, is oxidized by the radical products of lignin and manganese peroxidases, whereas cellulose and hemicellulose are hydrolysed. Both peroxidases and cellulases exist as complex families at both the DNA and protein levels. The lignin peroxidases may function principally when mycelium-bound and, therefore, undetectable in culture supernatants. Moreover, methods for the study of P. chrysosporium must be applicable to solid substrate as well as liquid-culture conditions. For these reasons, detailed studies of gene expression, made possible by the reverse transcriptase-polymerase chain reaction method, are essential. Such studies reveal that gene families are subject to differential expression. The cellulase system has some differences from that of Trichoderma reesei; the distinction made between the activities of exocellobiohydrolases and endoglucanases needs to be re-appraised in both species. Current studies also seek to reconstruct the systems of degradation of lignocellulose and its individual components by heterologous expression of individual proteins in recombinant systems, and their use in mechanistic studies singly and in combinations.

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Temporal expression of the major lignin peroxidase genes of Phanerochaete chrysosporium

Cited by 23 publications

References 18 publications

PCR-mediated analysis of lignocellulolytic gene transcription by Phanerochaete chrysosporium: substrate-dependent differential expression within gene families

PCR-mediated analysis of lignocellulolytic gene transcription by Phanerochaete chrysosporium: substrate-dependent differential expression within gene families

Expression of lip genes during growth in soil and oxidation of anthracene by Phanerochaete chrysosporium

Lignocellulose degradation by Phanerochaete chrysosporium: gene families and gene expression for a complex process

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