2018
DOI: 10.1017/s0967199418000175
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Temporal expression of pluripotency-associated transcription factors in sheep and cattle preimplantation embryos

Abstract: SummaryPluripotency-associated transcription factors (PATFs) modulate gene expression during early mammalian embryogenesis. Despite a strong understanding of PATFs during mouse embryogenesis, limited progress has been made in ruminants. This work aimed to describe the temporal expression of eight PATFs during both sheep and cattle preimplantation development. Transcript availability of PATFs was evaluated by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) in eggs, cleavage-stage embryos,… Show more

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Cited by 9 publications
(8 citation statements)
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References 52 publications
(68 reference statements)
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“…aries inter-genus RT-qPCR as assessed by five algorithms. Fourthly, some primers have been used for intra-specific RT-PCR in eggs and early embryos of livestock species [42,43].…”
Section: Discussionmentioning
confidence: 99%
“…aries inter-genus RT-qPCR as assessed by five algorithms. Fourthly, some primers have been used for intra-specific RT-PCR in eggs and early embryos of livestock species [42,43].…”
Section: Discussionmentioning
confidence: 99%
“…COCs with homogeneous cytoplasm and at least one compact layer of cumulus cells were selected for in vitro maturation (IVM), as previously described by Silva et al (2018). The COCs were incubated at 38.5°C, under 5% CO 2 in air and saturated humidity for 24 h.…”
Section: Oocyte Collection and In Vitro Maturationmentioning
confidence: 99%
“…Denuded oocytes were washed in synthetic oviduct fluid (SOF) medium and subjected to PA by 5.0 μM ionomycin in SOF medium for 5 min and washed once in SOF medium. Next, oocytes were incubated in 2 mM 6-dimethylaminopurine (6-DMAP) for 3 h as previously described by Silva et al (2018). Activated oocytes were extensively washed in SOF medium and transferred to a culture dish containing a cumulus cell monolayer.…”
Section: Parthenogenetic Activation and Embryo Culturementioning
confidence: 99%
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