Temporal detection of Lawsonia intracellularis using serology and real-time PCR in Thoroughbred horses residing on a farm endemic for equine proliferative enteropathy

Pusterla, Nicola; Jackson, Ryan; Robert, Wilson; Collier, Jo; Mapes, S.; Gebhart, Connie J.

doi:10.1016/j.vetmic.2008.10.004

Cited by 35 publications

(32 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Here, relying on a large field study with naturally occurring cases, we have examined the effect of passively acquired antibodies and found that they do not significantly affect the occurrence of presumptive clinical or subclinical EPE. Given that passively acquired IgG is short‐lived , that IgG is the predominant immunoglobulin in colostrum and hyperimmune plasma products, and that L. intracellularis infection is not a systemic infection but rather a localised, mucosal infection, it is not surprising that no significant effect of passively acquired antibodies was noted in this study. Other potential reasons for weanling predisposition to EPE continue to include management changes associated with weaning and the post weaning period (including stress), loss of maternally derived nutrition and immunological factors (i.e.…”

Section: Discussionmentioning

confidence: 68%

The effect of passively acquired antibodies onLawsonia intracellularisinfection and immunity in the horse

Page

Stills

Horohov

2014

Equine Veterinary Journal

View full text Add to dashboard Cite

This is the first study to show that passively acquired antibodies to L. intracellularis do not have an effect on the occurrence of clinical or subclinical EPE. A number of novel findings, including identification of the disease rate among naturally exposed horses, warrant additional work as they may help to identify potential risk factors for L. intracellularis exposure and/or the reservoir host(s) of the bacterium.

show abstract

Section: Discussionmentioning

confidence: 68%

The effect of passively acquired antibodies onLawsonia intracellularisinfection and immunity in the horse

Page

Stills

Horohov

2014

Equine Veterinary Journal

View full text Add to dashboard Cite

show abstract

“…Equine proliferative enteropathy was first reported in horses in 1982 by Duhamel and Wheeldon (1982). Since 1996, several reports of sporadic cases and outbreaks on breeding farms have been described (Williams et al, 1996;Frank et al, 1998;Brees et al, 1999;Lavoie et al, 2000;Schumacher et al, 2000;Bihr, 2003;McClintock and Collins, 2004;Deprez et al, 2005;Dauvillier et al, 2006;Sampieri et al, 2006;Wuersch et al, 2006;Feary et al, 2007;Frazer, 2008;McGurrin et al, 2008;Pusterla et al, 2008Pusterla et al, , 2009aGuimarães-Ladeira et al, 2009;Merlo et al, 2009;Shimizu et al, 2010;Van Den Wollenberg et al, 2011). In the last few years, reported cases of EPE have been increasing, occurring primarily in post-weaning foals and occasionally in adult horses.…”

Section: Etiologymentioning

confidence: 99%

“…In these cases, abdominocentesis will yield a non-inflammatory transudate. An ante-mortem diagnosis is generally confirmed via PCR detection of L. intracellularis in feces or rectal swab and/or serology (Pusterla et al, 2009a).…”

Section: Diagnosismentioning

confidence: 99%

Lawsonia intracellularis infection and proliferative enteropathy in foals

Pusterla

Gebhart

2013

Veterinary Microbiology

Self Cite

View full text Add to dashboard Cite

Equine proliferative enteropathy (EPE) is a disease of foals caused by the obligate intracellular organism Lawsonia intracellularis. This organism is unique in that it causes proliferation of infected enterocytes, resulting in thickening of the intestinal epithelium, most often the small intestine. This disease affects mainly weanling foals and causes fever, lethargy, peripheral edema, diarrhea, colic and weight loss. The diagnosis of EPE may be challenging and relies on the presence of hypoproteinemia, thickening of segments of the small intestinal wall observed on abdominal ultrasonography, positive serology and molecular detection of L. intracellularis in feces. The epidemiology and genetic basis for pathogenesis for this disease is beginning to be elucidated. Phenotypic traits, genomic features, and gene expression profiles during L. intracellularis infection in vitro and in vivo are presented. In addition, this article reviews the epidemiology, pathological and clinicopathological findings, diagnosis, and control of EPE.

show abstract

“…However, the first serum dilution used by that author was 1:30, while serological tests in our lab were 1:60, as this dilution showed the least nonspecific labeling. Epidemiological investigations on farms with clinical cases indicate that 10-65% of healthy adult horses and foals are seropositive for L. intracellularis (Frazer 2008, Pusterla et al 2009). The percentage of positive animals ranged from 3.57% to 16.67% in Minas Gerais herds, while the properties had no cases of clinical disease, which justifies lower rates of seropositive (Guimarães-Ladeira et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Equine proliferative enteropathy on a Brazilian farm

et al. 2015

View full text Add to dashboard Cite

ABSTRACT. - Lawsonia intracellularis infection on a horse farm in the Midwest region of Brazil is described. Thirty-nine foals a few days to months old from a herd with 300 horses, experienced diarrhea with variable characteristics and intensities, weight loss, hyperemic mucous membranes and dehydration. In foals 3 to 6 months of age, hypoproteinemia associated with submandibular edema were also common. Intestinal fragments of a 7-month-old foal were sent to an animal disease laboratory for diagnosis. The observed macroscopic lesions were hyperemic serosa, thickening of the intestinal wall with a corrugation, thickening of the mucosa folds and reduction of intestinal lumen. Histological analysis of the small and large intestine revealed enterocyte hyperplasia of the crypts associated with diffuse marked decrease in the number of goblet cells and positive L. intracellularis antigen labeling by immunohistochemistry. Three out of 11 animals of the same property were seropositive for L. intracellularis, demonstrating the circulation of the agent throughout the farm, but none were PCR positive in fecal samples. Based on clinical signs and pathological findings, the diagnosis of equine proliferative enteropathy was confirmed.

show abstract

Temporal detection of Lawsonia intracellularis using serology and real-time PCR in Thoroughbred horses residing on a farm endemic for equine proliferative enteropathy

Cited by 35 publications

References 7 publications

The effect of passively acquired antibodies onLawsonia intracellularisinfection and immunity in the horse

The effect of passively acquired antibodies onLawsonia intracellularisinfection and immunity in the horse

Lawsonia intracellularis infection and proliferative enteropathy in foals

Equine proliferative enteropathy on a Brazilian farm

Contact Info

Product

Resources

About