Temporal Coordination of Collective Migration and Lumen Formation by Antagonism between Two Nuclear Receptors

Wang, Heng; Liu, Lingli; Li, Sheng; Emery, Grégory; Chen, Jiong

doi:10.1016/j.isci.2020.101335

Cited by 8 publications

(6 citation statements)

References 62 publications

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“…These migration-related activities included regulation of the cytoskeleton, cell adhesion, small GTPase activity, and cell polarity. Cluster C0 had additional functional interaction modules with proteins required for septate junction formation and proteins involved in ecdysone signaling response, both of which are required for border cell migration ( Figure 4A ) [53; 25; 27; 54]. Cluster C8 was further enriched with modules of proteins with annotated broad functions in development, including cell signaling, serine-threonine kinase activities, and regulation of gene expression ( Figure 4C ).…”

Section: Resultsmentioning

confidence: 99%

Transcriptome analysis reveals temporally regulated genetic networks duringDrosophilaborder cell collective migration

Burghardt,

Rakijas,

Tyagi

et al. 2023

Preprint

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BackgroundCollective cell migration underlies many essential processes, including sculpting organs during embryogenesis, wound healing in the adult, and metastasis of cancer cells. At mid-oogenesis,Drosophilaborder cells undergo collective migration. Border cells round up into a small group, detach from the epithelium, and migrate – at first rapidly through the surrounding tissue, then slower, with the cluster rotating several times before stopping at the oocyte. While specific genes that promote cell signaling, polarization of the cluster, formation of protrusions, and cell-cell adhesion are known to regulate border cell migration, there may be additional genes that promote these distinct dynamic phases of border cell migration. Therefore, we sought to identify genes whose expression patterns changed during border cell migration.ResultsWe performed RNA-sequencing on border cells isolated at pre-, mid-, and late-migration stages. We report that 1,729 transcripts, in nine co-expression gene clusters, are temporally and differentially expressed across the three migration stages. Gene ontology analyses and constructed protein-protein interaction networks identified genes expected to function in collective migration, such as regulators of the cytoskeleton, adhesion, and tissue morphogenesis, but also a notable enrichment of genes involved in immune signaling, ribosome biogenesis, and stress responses. Finally, we validated thein vivoexpression and function of a subset of identified genes in border cells.ConclusionsOverall, our results identified differentially and temporally expressed genetic networks that may facilitate the efficient development and migration of border cells. The genes identified here represent a wealth of new candidates to investigate the molecular nature of dynamic collective cell migrations in developing tissues.

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Section: Resultsmentioning

confidence: 99%

Transcriptome analysis reveals temporally regulated genetic networks duringDrosophilaborder cell collective migration

Burghardt,

Rakijas,

Tyagi

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Cluster C0 had additional functional interaction modules with proteins required for septate junction formation and proteins involved in ecdysone signaling response, both of which are required for border cell migration (Fig. 5 A) [ 26 , 28 , 55 , 56 ]. Cluster C8 was further enriched with modules of proteins with annotated broad functions in development, including cell signaling, serine-threonine kinase activities, and regulation of gene expression (Fig.…”

Section: Resultsmentioning

confidence: 99%

Transcriptome analysis reveals temporally regulated genetic networks during Drosophila border cell collective migration

Burghardt,

Rakijas,

Tyagi

et al. 2023

BMC Genomics

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Background Collective cell migration underlies many essential processes, including sculpting organs during embryogenesis, wound healing in the adult, and metastasis of cancer cells. At mid-oogenesis, Drosophila border cells undergo collective migration. Border cells round up into a small group at the pre-migration stage, detach from the epithelium and undergo a dynamic and highly regulated migration at the mid-migration stage, and stop at the oocyte, their final destination, at the post-migration stage. While specific genes that promote cell signaling, polarization of the cluster, formation of protrusions, and cell-cell adhesion are known to regulate border cell migration, there may be additional genes that promote these distinct active phases of border cell migration. Therefore, we sought to identify genes whose expression patterns changed during border cell migration. Results We performed RNA-sequencing on border cells isolated at pre-, mid-, and post-migration stages. We report that 1,729 transcripts, in nine co-expression gene clusters, are temporally and differentially expressed across the three migration stages. Gene ontology analyses and constructed protein-protein interaction networks identified genes expected to function in collective migration, such as regulators of the cytoskeleton, adhesion, and tissue morphogenesis, but also uncovered a notable enrichment of genes involved in immune signaling, ribosome biogenesis, and stress responses. Finally, we validated the in vivo expression and function of a subset of identified genes in border cells. Conclusions Overall, our results identified differentially and temporally expressed genetic networks that may facilitate the efficient development and migration of border cells. The genes identified here represent a wealth of new candidates to investigate the molecular nature of dynamic collective cell migrations in developing tissues.

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“…Ce modèle a révélé le haut degré de complexité nécessaire à la régulation de la migration collective. À côté des processus que nous avons décrits, l'implication d'autres voies de signalisation a été mise en évidence dans des processus aussi divers que la différenciation des cellules de bordure, la cinétique de leur migration [49,50], et l'ancrage des cellules après leur migration [51].…”

Section: Perspectivesunclassified

Je mène donc tu suis

Emery

2023

Med Sci (Paris)

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Lors du développement et de la cicatrisation, les cellules se déplacent souvent par un processus de « migration cellulaire collective ». Un procédé identique est employé par les cellules de certaines tumeurs cancéreuses lors de la formation de métastases. Un remarquable modèle d’étude de la migration cellulaire collective est celui de l’étude du groupe (cluster) de cellules de bordure de la drosophile, qui permet d’observer et de manipuler une migration collective dans son environnement naturel. Cette revue décrit la machinerie moléculaire qui permet à ce groupe de cellules de migrer directionnellement, en se concentrant sur les mécanismes permettant aux cellules de détecter et réagir aux chimioattractants et d’organiser le groupe en cellules leaders et suiveuses.

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Temporal Coordination of Collective Migration and Lumen Formation by Antagonism between Two Nuclear Receptors

Cited by 8 publications

References 62 publications

Transcriptome analysis reveals temporally regulated genetic networks duringDrosophilaborder cell collective migration

Transcriptome analysis reveals temporally regulated genetic networks duringDrosophilaborder cell collective migration

Transcriptome analysis reveals temporally regulated genetic networks during Drosophila border cell collective migration

Je mène donc tu suis

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