Temporal and spatial expression and function of TaDlea3 in Triticum aestivum during developmental stages under drought stress

Chen, Juan; Fan, Lei; Du, Yanjun; Zhu, Weining; Tang, Ziqin; Li, Na; Zhang, David; Zhang, Linsheng

doi:10.1016/j.plantsci.2016.08.010

Cited by 7 publications

(6 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Environmental stresses, such as drought, low temperature and high salinity, affect plant growth by disturbing the water balance. Many studies have demonstrated that LEA proteins play crucial roles in reducing the damage caused by environmental stress (Xing et al, 2011;Chen et al, 2016;Yu et al, 2019). ZmDHN11 belongs to the group II LEA protein family, also known as dehydrin.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of ZmDHN11 could enhance transgenic yeast and tobacco tolerance to osmotic stress

et al. 2021

Plant Cell Rep

View full text Add to dashboard Cite

Late embryogenesis abundant (LEA) proteins are widely assumed to play crucial roles in environmental stress tolerance, but their function has remained obscure. Dehydrins are group II LEA proteins, which are highly hydrophilic plant stress proteins. In the present study, a novel group II LEA protein, ZmDHN11 was cloned and identi ed from maize. The expression of ZmDHN11 was induced by high osmotic stress, low temperature, salinity and ABA (abscisic acid). The ZmDHN11 protein speci cally accumulated in the nuclei and cytosol. Further study indicated that ZmDHN11 is phosphorylated by the casein kinase CKII.ZmDHN11 protected the activity of LDH under water de cit stress. The overexpression of ZmDHN11 endows transgenic yeast and tobacco with tolerance to osmotic stress. Key MessageMaize group II LEA protein ZmDHN11 could protect protein activity and confer resistance to osmotic stress on transgenic yeast and tobacco.

show abstract

Section: Discussionmentioning

confidence: 99%

Overexpression of ZmDHN11 could enhance transgenic yeast and tobacco tolerance to osmotic stress

et al. 2021

Plant Cell Rep

View full text Add to dashboard Cite

show abstract

“…Subcellular localization was determined as described in previous research [ 58 ]. We cloned AhbHLH112 into a pCAMBIA2300-GFP donor vector at the XbaI and SalI restriction sites and generated a pCAMBIA2300-AhbHLH112-GFP plasmid.…”

Section: Methodsmentioning

confidence: 99%

“…Each experiment was performed with three biological replicates. The expression levels of antioxidant genes and ABA response genes were normalized against those of UBC , which was used as an internal CK [ 58 ]. The reactions were performed as mentioned above.…”

Section: Methodsmentioning

confidence: 99%

The bHLH transcription factor AhbHLH112 improves the drought tolerance of peanut

Chunjuan¹,

Cheng²,

Sun³

et al. 2021

BMC Plant Biol

View full text Add to dashboard Cite

Background Basic helix-loop-helix (bHLH) transcription factors (TFs) are one of the largest gene families in plants. They regulate gene expression through interactions with specific motifs in target genes. bHLH TFs are not only universally involved in plant growth but also play an important role in plant responses to abiotic stress. However, most members of this family have not been functionally characterized. Results Here, we characterized the function of a bHLH TF in the peanut, AhHLH112, in response to drought stress. AhHLH112 is localized in the nucleus and it was induced by drought stress. The overexpression of this gene improves the drought tolerance of transgenic plants both in seedling and adult stages. Compared to wild-type plants, the transgenic plants accumulated less reactive oxygen species (ROS), accompanied by increased activity and transcript levels of antioxidant enzymes (superoxide dismutase, peroxidase and catalase). In addition, the WT plants demonstrated higher MDA concentration levels and higher water loss rate than the transgenic plants under drought treatment. The Yeast one-hybrid result also demonstrates that AhbHLH112 directly and specifically binds to and activates the promoter of the peroxidase (POD) gene. Besides, overexpression of AhHLH112 improved ABA level under drought condition, and elevated the expression of genes associated with ABA biosynthesis and ABA responding, including AtNCED3 and AtRD29A. Conclusions Drawing on the results of our experiments, we propose that, by improving ROS-scavenging ability, at least in part through the regulation of POD -mediated H2O2 homeostasis, and possibly participates in ABA-dependent stress-responding pathway, AhbHLH112 acts as a positive factor in drought stress tolerance.

show abstract

“…Subcellular localization was performed with Ag. tumefaciens infiltration with Nicotiana tabacum leaves, as described by Chen et al (2016).…”

Section: Genetic Transformation Of Arabidopsis Thaliana and Subcellular Localizationmentioning

confidence: 99%

“…For drought treatment, the watering was stopped immediately and no water added for 14 days. Superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and malondialdehyde (MDA) activity of WT and transgenic Arabidopsis plants under drought stress were measured as described by Chen et al (2016).…”

Section: Determination Of Transgenic a Thaliana Drought Resistancementioning

confidence: 99%

Proteomic profiling of Arachis hypogaea in response to drought stress and overexpression of AhLEA2 improves drought tolerance

Li¹,

Cheng²,

Sun³

et al. 2021

Plant Biol J

View full text Add to dashboard Cite

Drought is the main factor restricting peanut growth, but the molecular mechanism underlying peanut drought tolerance remains unclear.• Herein, the seedling stage of drought-resistant peanut cultivar J11 was subjected to drought stress, and its proteomic profile was systematically analysed by isobaric tags for relative and absolute quantification (iTRAQ), the results of which were further complemented with our previous transcriptome results.• A total of 4,018 proteins were identified by proteomic analysis, which revealed that the expression levels of 69 proteins were altered under drought stress. Among the differentially expressed proteins (DEPs), 50 were upregulated, and 19 were downregulated. The most enriched metabolic pathways for these DEPs were those involving phenylpropanoid biosynthesis, flavonoid biosynthesis, and plant hormone signal transduction. The proteomic data and previous transcriptome results revealed 44 groups of genes/proteins with the same expression trend, including a LEA (Late embryogenesis abundant) gene, AhLEA2. Our present study showed that overexpression of the AhLEA2 gene enhanced the drought resistance of transgenic Arabidopsis plants, and the activities of related antioxidant enzymes in the transgenic plants significantly changed. The AhLEA2 gene was found to be located in the cytoplasm and cell membrane by subcellular localization experiments.• This work systematically analysed the differentially expressed proteins in peanut in response to drought stress, providing important candidates for further functional analysis of the stress response of peanut. Our results also indicated that AhLEA2 plays an important role in the peanut response to drought stress.

show abstract

Temporal and spatial expression and function of TaDlea3 in Triticum aestivum during developmental stages under drought stress

Cited by 7 publications

References 46 publications

Overexpression of ZmDHN11 could enhance transgenic yeast and tobacco tolerance to osmotic stress

Overexpression of ZmDHN11 could enhance transgenic yeast and tobacco tolerance to osmotic stress

The bHLH transcription factor AhbHLH112 improves the drought tolerance of peanut

Proteomic profiling of Arachis hypogaea in response to drought stress and overexpression of AhLEA2 improves drought tolerance

Contact Info

Product

Resources

About