Telomere length, chromatin structure and chromosome fusigenic potential

Slijepčević, Predrag; Hande, M. Prakash; Bouffler, Simon; Lansdorp, Peter M.; Bryant, Peter E.

doi:10.1007/s004120050263

Cited by 83 publications

(67 citation statements)

References 36 publications

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“…The fusion of chromosome fragments as revealed by ITS appearance is frequently observed in irradiated mammalian somatic cells (Slijepcevic et al, 1997). In the present study, the chromosome fragments missing centromeres might have to undergo a process of DNA incorporation into intact chromosomes.…”

Section: Discussionmentioning

confidence: 53%

The stability of telomereless chromosome fragments in adult androgenetic rainbow trout

Ocalewicz

Babiak

Dobosz

et al. 2004

Journal of Experimental Biology

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SUMMARY The study provides new data on the stability of gamma radiation-induced chromosome fragments of a putative maternal nuclear genome in an androgenetic vertebrate, rainbow trout (Oncorhynchus mykiss Walbaum). The fragments were found in five of 16 examined individuals and they were mostly centromeric parts of metacentric or subtelocentric chromosomes. Chromosome fragments were identical in all cells of a given androgenetic individual,indicating that segregation of chromosome fragments is active from the early cell divisions. Most of the fragments were telomereless, i.e. they had no telomeric sequences on their ends. This shows that telomeres are not necessary for stability of chromosomal structures in a vertebrate genome. In one individual, the interstitial telomeric sites were found in chromosomes, which could be the effect of joining chromosome fragments.

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Section: Discussionmentioning

confidence: 53%

The stability of telomereless chromosome fragments in adult androgenetic rainbow trout

Ocalewicz

Babiak

Dobosz

et al. 2004

Journal of Experimental Biology

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“…Numerous studies report increased incidence of anaphase bridges when TL and/or integrity is compromised (33)(34)(35)(36); however, to our knowledge, evidence of telomeric DNA amplification through this mechanism has not been reported. DC formation is a known initiator of BFB cycling, gene amplification, and altered gene dosage (34,36).…”

Section: Bfb Cycling Is Not Responsible For Amplification Of Telomerimentioning

confidence: 56%

Folate Deficiency Induces Dysfunctional Long and Short Telomeres; Both States Are Associated with Hypomethylation and DNA Damage in Human WIL2-NS Cells

Bull

Mayrhofer

O’Callaghan

et al. 2014

Cancer Prevention Research

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The essential role of dietary micronutrients for genome stability is well documented, yet the effect of folate deficiency or excess on telomeres is not known. Accordingly, human WIL2-NS cells were maintained in medium containing 30, 300, or 3,000 nmol/L folic acid (FA) for 42 days to test the hypothesis that chronic folate deficiency would cause telomere shortening and dysfunction. After 14 days, telomere length (TL) in FA-deficient (30 nmol/L) cultures was 26% longer than that of 3,000 nmol/L FA cultures; however, this was followed by rapid telomere attrition over the subsequent 28 days (P trend, P < 0.0001); both long and short telomere status was positively correlated with biomarkers of chromosome instability (P 0.003) and mitotic dysfunction (P ¼ 0.01), measured by the cytokinesis-block micronucleus cytome (CBMN-cyt) assay. The early increase in TL was associated with FA-deficiency-induced global DNA hypomethylation (P ¼ 0.05), with an effect size similar to that induced by the DNA methyltransferase inhibitor, 5-aza-2 0 -deoxycytidine. Quantitative PCR analysis indicated a negative association between FA concentration and uracil incorporation into telomeric DNA (r ¼ À0.47, P ¼ 0.1), suggesting a possible plausible mechanism for uracil as a cause of folate deficiency-induced telomere dysfunction or deletion. Peptide nucleic acid-FISH (PNA-FISH) analysis showed that FA deficiency resulted in 60% of micronuclei containing acentric terminal fragments, an observation consistent with the 3-fold increase in terminal deletions (P ¼ 0.0001). Together, these results demonstrate the impact of folate deficiency on biomarkers of telomere maintenance and integrity, and provide evidence that dysfunctional long telomeres may be as important as critically short telomeres as a cause of chromosomal instability. Cancer Prev Res; 7(1); 128-38. Ó2013 AACR.

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“…Los telómeros alargados que carecen de ADN normal o marcas de metilación de histonas también resultan en un aumento de recombinaciones teloméricas (20). Aunque es la estructura de la cromatina la determinante de la recombinación, los telómeros más largos experimentan recombinaciones con más frecuencia que los más cortos (20,21).…”

Section: Introductionunclassified

Telómeros Y Calidad De La Dieta: Una Revision Sistematica

Marti¹,

Echeverría

Morell‐Azanza

et al. 2017

Nutr Hosp

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Palabras clave:Telómeros. Dieta mediterránea. Estrés oxidative. ADN. ResumenFundamento: existen pocos estudios que hayan evaluado la relación entre la calidad de la dieta y la integridad telómerica en humanos. Los telómeros son regiones de ADN no codificante que se encuentran en los extremos de los cromosomas, cuya longitud además de indicar la esperanza de vida, indica el estado global de salud. El objetivo de la presente revisión sistemática es recopilar la evidencia existente sobre la relación entre la longitud de los telómeros y la calidad de la dieta para conocer el impacto que algunos nutrientes, alimentos y patrones dietéticos pueden tener sobre la homeostasis telomérica y por lo tanto, sobre la salud en general. Material y métodos: se realizó una revisión bibliográfica en la base de datos PubMed para identificar los artículos publicados (en inglés o español) hasta diciembre de 2016 que cumplían los siguientes criterios: incluían sujetos humanos; eran estudios transversales; estudios de casos y controles; estudios de cohortes prospectivos o estudios de intervención; que evaluaban la relación de los nutrientes, alimentos o patrones dietéticos con la longitud de los telómeros. La estrategia de búsqueda incluía las siguientes palabras clave: nutrients or food OR food groups OR diet OR dietary pattern OR eating pattern OR dietary habits OR diet type AND telomere attrition OR telomere length. En total, se incluyeron 19 estudios transversales, cinco estudios de casos y controles, cinco estudios de cohortes prospectivos y dos estudios de intervención, incluyendo aquellos artículos que se encontraron por listas de referencias de otras publicaciones. Resultados: se encontraron asociaciones positivas entre la longitud de los telómeros y la adherencia a la dieta mediterránea y el consumo de verduras y frutas. Los resultados obtenidos para otros nutrientes, alimentos o patrones dietéticos fueron incoherentes, aunque parece que las carnes procesadas, los cereales, el alcohol y las bebidas endulzadas podrían estar asociados con telómeros más cortos. Conclusiones: la intervención dietética, y en particular la promoción de una dieta de estilo mediterráneo, podría desempeñar un papel en la protección de la integridad telomérica. Key words:Telomere. Mediterranean diet. Oxidative stress. DNA. AbstractBackground: Few studies have evaluated the relationship between diet quality and telomere integrity in humans. Telomeres are regions of non-coding DNA localized at the end of each chromosome whose length, in addition to indicating life expectancy, indicates an overall health status. The objective of this systematic review is to compile the existing evidence on the relationship between telomere length and diet quality to further explore the impact that some nutrients, foods and dietary patterns may have on telomere homeostasis and therefore, in precision nutrition strategies. Material and methods: A bibliographic review was performed in the PubMed database to identify published articles (in English or Spanish) until December 201...

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Telomere length, chromatin structure and chromosome fusigenic potential

Cited by 83 publications

References 36 publications

The stability of telomereless chromosome fragments in adult androgenetic rainbow trout

The stability of telomereless chromosome fragments in adult androgenetic rainbow trout

Folate Deficiency Induces Dysfunctional Long and Short Telomeres; Both States Are Associated with Hypomethylation and DNA Damage in Human WIL2-NS Cells

Telómeros Y Calidad De La Dieta: Una Revision Sistematica

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