Techniques for Analysis of Plant Phenolic Compounds

Khoddami, Ali; Wilkes, Meredith; Roberts, Thomas H.

doi:10.3390/molecules18022328

Cited by 1,011 publications

(777 citation statements)

References 289 publications

Supporting

Mentioning

685

Contrasting

Unclassified

Order By: Relevance

“…The amount of SA derivatives (which include sinapoyl glucose, methyl sinapate, sinapoyl malate and 1-sinapoyl-2-feruloylgentiobiose) found in rapeseed ranges between 6,390 and 18,370 μg/g, but this is dependent on the method used to process the oil (Lomascolo et al, 2012;Nićiforović and Abramovič, 2014). walls and release of the bioactive contents (Khoddami et al, 2013). Teh and Birch (2014) previously determined the optimal parameters to extract phenolics from defatted canola seed cakes to comprise a 200 W ultrasound extraction with a solvent volume of 50 mL, extraction time of 20 min, and temperature of 70°C.…”

Section: Sources and Derivatives Of Samentioning

confidence: 99%

Sinapinic and protocatechuic acids found in rapeseed: isolation, characterisation and potential benefits for human health as functional food ingredients

Quinn

Gray

Meaney³

et al. 2017

Irish Journal of Agricultural and Food Research

View full text Add to dashboard Cite

Rapeseed is one of the world’s major oilseeds, and rapeseed oil is produced by pressing of the seeds. This process results in the production of a low-economic-value by-product, rapeseed meal, which is commonly used as animal feed. Rapeseed meal is rich in bioactive phenolic compounds, including sinapinic acid (SA) and protocatechuic acid (PCA). Isolation of these bioactive compounds from a by-product of rapeseed oil production is largely in agreement with the current concept of the circular economy and total utilisation of crop harvest using a biorefinery approach. In this review, current information concerning traditional and novel methods to isolate phenolic compounds – including SA and PCA – from rapeseed meal, along with in vitro and in vivo studies concerning the bioactivity of SA and PCA and their associated health effects, is collated. These health effects include anti-inflammatory, anti-cancer, anti-diabetes activities, along with histone deacetylase inhibition and protective cardiovascular, neurological and hepatic effects. The traditional extraction methods include use of solvents and/or enzymes. However, a need for simpler, more efficient methodologies has led to the development of novel extraction processes, including microwave-assisted, ultrasound-assisted, pulsed electric field and high-voltage electrical discharge extraction processes.

show abstract

Section: Sources and Derivatives Of Samentioning

confidence: 99%

Sinapinic and protocatechuic acids found in rapeseed: isolation, characterisation and potential benefits for human health as functional food ingredients

Quinn

Gray

Meaney³

et al. 2017

Irish Journal of Agricultural and Food Research

View full text Add to dashboard Cite

show abstract

“…These compounds were a phytochemicals group which have a phenol structure and considered as important determinants in the nutritional and the quality of fruits, vegetables, other plant foods, and food preparations. Phenolic compounds were widely found in the plant world and commonly involved in defense toward ultraviolet radiation or attack by pathogens, parasites, and predators [2]. Hence, these compounds play an important role in plant growth and offer the plants to have excellent properties as food preservatives.…”

Section: Introductionmentioning

confidence: 99%

Extraction of Phytochemicals from Grains of Paradise Using Supercritical Carbon Dioxide

Ono¹,

Kawamoto²,

Uemori³

et al. 2017

View full text Add to dashboard Cite

Abstract. Supercritical carbon dioxide (SCCO2) is one of the green methods to extract plant matters substances. In this study, SCCO2 has been applied as a media to extract the grains of paradise seeds substances at temperatures of 40 -80 o C and pressures of 20 -40 MPa using a semi-continuous flow-type system. Results of FT-IR spectra analysis showed that the grains of paradise seeds substances were extracted by SCCO2. The SEM images of the grains of paradise seeds residues also showed that the physical changes of the grains of paradise seeds surface occurred after SCCO2 extraction. The main compounds in extracts were gingerols, shogaols, and paradols. However, the GC-MS may only identify paradol compounds due to gingerols, shogaols, and paradols are homologous series of phenolic ketones and paradols was more stable than gingerols and shogaols. The yields of gingerols, shogaols, and paradols were 9.12, 3.90, and 4.42 mg/g sample, respectively and affected by extraction temperatures and/or extraction pressures.

show abstract

“…The most advanced technique used today is high performance liquid chromatography (HPLC) coupled with mass spectrometry (MS), a powerful identification technique, based on specific fragmentation of each separated molecule (Socaciu et al, 2009;Mattoli et al, 2011;Khoddami et al, 2013). Although organic solvent (methanol) extraction is the main method used to extract phenolics, for medical studies aqueous extraction using microwaves or ultrasounds is an ecologic alternative.…”

Section: Introductionmentioning

confidence: 99%

Comparative HPLC-DAD-ESI(+)MS Fingerprint and Quantification of Phenolic and Flavonoid Composition of Aqueous Leaf Extracts of <i>Cornus mas<i> and <i>Crataegus monogyna</i>, in Relation to Their Cardiotonic Potential

Badalica-Petrescu

Drăgan

Ranga

et al. 2014

Not Bot Hort Agrobot Cluj

View full text Add to dashboard Cite

Our study aimed to compare the phenolic and flavonoid composition of two medicinal plants from the wild flora of Romania, traditionally known to be efficient in preventing cardiotoxicity: Cornus mas and Crataegus monogyna. As most previous studies have focused on fruits, our investigations aimed to fingerprint and quantify the two classes of compounds in aqueous leaf extracts, based on HPLC-DAD-ESI(+)MS analysis. The specific HPLC-DAD fingerprint was coupled with UV spectra at 280 and 340 nm to discriminate between three subclasses of compounds: two hydroxycinnamic acids and a flavonoid glycoside. While the C. monogyna extract contained more than 98% vitexin isomers (2'-and 4'-O-rhamnoside), the C. mas extract was very complex, containing a mixture of phenolic derivatives and flavonoid glycosides. The spectral patterns of C. mas, combined with the molecular mass and specific fragmentations allowed the identification of epi-catechin, coumaric and caffeic acids and quercetin derivatives. Based on LC-MS peak area and parallel calibrations with gallic acid and rutin, the mean concentration for flavonoids in C. mas was 13 mg/100 ml, while aprox. 65 mg/100 ml in C. monogyna extracts. Using the Folin method, the total phenol content was 105 mg/100 ml in C. mas and around three times less in the C. monogyna extract, while the antioxidant activity was increased only 1.5-1.7 times in C. mas extract compared to C. monogyna. These findings suggest a higher stability and potential of flavonoids to act as antioxidants in hydrophilic environment.

show abstract

Techniques for Analysis of Plant Phenolic Compounds

Cited by 1,011 publications

References 289 publications

Sinapinic and protocatechuic acids found in rapeseed: isolation, characterisation and potential benefits for human health as functional food ingredients

Sinapinic and protocatechuic acids found in rapeseed: isolation, characterisation and potential benefits for human health as functional food ingredients

Extraction of Phytochemicals from Grains of Paradise Using Supercritical Carbon Dioxide

Comparative HPLC-DAD-ESI(+)MS Fingerprint and Quantification of Phenolic and Flavonoid Composition of Aqueous Leaf Extracts of <i>Cornus mas<i> and <i>Crataegus monogyna</i>, in Relation to Their Cardiotonic Potential

Contact Info

Product

Resources

About