Technical and surgical aspects of continuous vascular access in freely moving small animals

Gebhardt, J; Hinrichs, Cedric; Fändrich, F.; Schröder, Jörg; Bautz, Andrea

doi:10.1016/s0022-2143(97)90042-1

Cited by 5 publications

(4 citation statements)

References 13 publications

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“…15) The intravenous catheter, which was brought out to the back of the head, was connected to the end of the swivel joint through a leather harness, which allowed free movement of the rats during the continuous vascular access for as long as 3 weeks.…”

Section: Surgical Preparationmentioning

confidence: 99%

Is Absorption Profile of Cyclosporine Really Important for Effective Immunosuppression?

Kobayashi

Kuzuya

et al. 2006

Biological & Pharmaceutical Bulletin

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The clinical significance of cyclosporine (CsA) concentration 2 h postdose (C 2 ) monitoring is widely recognized in organ transplantation, because C 2 value is considered to be a predictable surrogate marker of full area under the concentration-time curve (AUC), and/or a peak concentration value exhibits potent inhibition of calcineurin activity. However, the pharmacological advantage of absorption profile (AP) has not been fully elucidated. In a rat skin allotransplantation model, the authors evaluated the efficacy of AP by different dosage regimens (20, 25 or 30 mg/kg/d, once or twice daily) and routes (p.o. or i.v.), and examined whether high C 2 or AUC 0-4 is intrinsically valuable for effective immunosuppression. Graft survival was CsA dose-dependent and correlated with full AUC 0-24 , rather than AP. The difference between the once and twice daily administrations did not influence full AUC 0-24 or immunosuppressive effect. Continuous intravenous infusion with flat pharmacokinetics also produced adequate immunosuppression as was observed in enteral administration at the same level of total exposure. The impact of high peak concentration in AP on immunosuppressive effect could not be found. It was suggested that AP would not have intrinsic pharmacodynamic value. However, absorption profiling was considered to be clinically useful in that C 2 value is a good surrogate marker of total exposure (AUC 0-24 ).

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Section: Surgical Preparationmentioning

confidence: 99%

Is Absorption Profile of Cyclosporine Really Important for Effective Immunosuppression?

Kobayashi

Kuzuya

et al. 2006

Biological & Pharmaceutical Bulletin

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“…Two fine catheters (3 Fr, inner diameter: 0.6 mm, outer diameter: 1.0 mm, Atom Medical International, Tokyo, Japan) were introduced deeply into the right external jugular vein and the right carotid artery individually as previously described. 18,19 Then, a trocar was inserted subcutaneously from the dorsal aspect of the midcervical region exiting at the ventral skin incision on the neck. 19 The external ends of both catheters were threaded through the trocar to exit at the scapula, the trocar was withdrawn, and the wound was closed.…”

Section: Surgical Procedures For the Establishment Of Extra-corporealmentioning

confidence: 99%

“…18,19 Then, a trocar was inserted subcutaneously from the dorsal aspect of the midcervical region exiting at the ventral skin incision on the neck. 19 The external ends of both catheters were threaded through the trocar to exit at the scapula, the trocar was withdrawn, and the wound was closed. A specially prepared miniature column filled with the SC or cellulose beads (1.0 ml, Toray Industries Inc. Tokyo, Japan) and an infusion pump (Peristaltic Pump MP-1100, EYELA, Tokyo, Japan) were connected between these catheters (Figure 1).…”

Section: Surgical Procedures For the Establishment Of Extra-corporealmentioning

confidence: 99%

Spherical Sulfated Cellulose Adsorbs High-Mobility-Group Box Chromosomal Protein 1 In Vitro and In Vivo

Suda¹,

Takeuchi²,

Hagiwara³

et al. 2010

ASAIO Journal

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High-mobility-group box chromosomal protein 1 (HMGB1) has recently been identified as a late mediator of various kinds of acute and chronic inflammation. A method for efficiently removing HMGB1 from systemic circulation could be a promising therapy for HMGB1-mediated inflammatory diseases. It is well known that the cationic portion of HMGB1 binds to heparin, which has abundant sulfates in its structure. In this study, we determined whether spherical sulfated cellulose (SC) efficiently adsorbed HMGB1, as well as other inflammatory mediators, in vitro. Then, we investigated the efficacy of hemoperfusion with the SC (SC group) or cellulose beads (control group) at adsorbing endogenous mediators, including HMGB1, in vivo. We have demonstrated that the SC adsorbed significantly larger amounts of HMGB1, interleukin (IL)-4, and IL-8 when compared with cellulose beads, in vitro. Hemoperfusion with the SC for 30 minute, starting 2 hour after an abdominal opening and closure operation, significantly reduced serum HMGB1 levels (p = 0.004) and consistently increased serum IL-10 levels, in vivo. These data suggest the potential benefits of hemoperfusion using the SC in treating HMGB1-mediated inflammatory diseases.

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“…Hence, various systems and techniques for continuous vascular access have been developed. Tethered infusion systems including a jacket/ harness (Gebhardt et al, 1997) and tail cuff (Cave et al, 1995;Wijk et al, 2000;Asanuma et al, 2006) are well used in rodents but require additional equipment (swivels, external catheters, lever arms, etc.) and enough space where these items set up.…”

Section: Introductionmentioning

confidence: 99%

Application of an indwelling vascular access port for intravenous administration in a repeated and intermittent dose toxicity study in rats

et al. 2009

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-Totally implantable catheter animal models are considered useful for pharmacological and toxicological studies. In this report, we assessed the feasibility of using an indwelling vascular access port (VAP) in rats for long-term evaluation of repeated and intermittent dose toxicity studies. In Experiment 1, the VAP devices were implanted in male and female rats and a saline solution administered intravenously via the posterior vena cava for 2 weeks (4 ml/kg, 2 ml/min, 5 times/week, 10 times total). General conditions, body weight and blood chemistry showed no toxicological changes compared with the rats in the non-implanted, non-treated group. Hematology changes such as transient increases in peripheral blood reticulocytes and eosinophils were noted post-implantation. In pathology, proliferation of the -nophils in lung were noted at the end of the treatment period. Moreover, we found that the lumbar area is more suitable for VAP implantation than the back of neck for young, still growing rats. Experiment 2 included a 1-month intravenous intermittent dose (4 ml/kg, 2 ml/min, 1 time/week, 5 times total) toxicity study in VAP-implanted rats followed by a 1-month recovery period conducted under Good Laboratory Practice (GLP) regulations. The results suggested that an animal model with implanted VAP is useful for intermittent intravenous dosing of drugs. Moreover, VAP implantation in animals is expected to be extrapolated to use VAP in humans in clinical studies.

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Technical and surgical aspects of continuous vascular access in freely moving small animals

Cited by 5 publications

References 13 publications

Is Absorption Profile of Cyclosporine Really Important for Effective Immunosuppression?

Is Absorption Profile of Cyclosporine Really Important for Effective Immunosuppression?

Spherical Sulfated Cellulose Adsorbs High-Mobility-Group Box Chromosomal Protein 1 In Vitro and In Vivo

Application of an indwelling vascular access port for intravenous administration in a repeated and intermittent dose toxicity study in rats

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