TcTASV Antigens of Trypanosoma cruzi: Utility for Diagnosis and High Accuracy as Biomarkers of Treatment Efficacy in Pediatric Patients

Floridia-Yapur, Noelia; Monje-Rumi, Mercedes; Ragone, Paula G.; Lauthier, Juan José; Tomasini, Nicolás; D'amato, A. Alberti; Diosque, Patricio; Cimino, Rubén O.; Gil, José Fernando; Sánchez, Daniel O.; Nasser, Julio R.; Tekiel, Valeria

doi:10.4269/ajtmh.18-0936

Cited by 7 publications

(12 citation statements)

References 18 publications

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“…Thirty-four studies were selected for the ELISA technique: [ 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 ] in which a total of 6054 subjects were studied. Sensitivity ranged from 78.0% to 100%, with a median of 99.0%, CI 95% (94, 100), while the test for equality of sensitivities presented a χ 2 = 657.24, df = 33, p -value = 2 × 10 −16 .…”

Section: Resultsmentioning

confidence: 99%

“…Sensitivity and specificity are reported with a mean (95% confidence limits). The Forest plot depicts the estimated sensitivity and specificity (black squares) and its 95% confidence limits (horizontal black line) [ 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 ].…”

Section: Figurementioning

confidence: 99%

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Accuracy of Diagnostic Tests for the Detection of Chagas Disease: A Systematic Review and Meta-Analysis

et al. 2022

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The present systematic review and meta-analysis about the accuracy of diagnostic tests aim to describe the findings of literature over the last thirty years for the diagnosis of Chagas disease (CD). This work aimed to determine the accuracy of diagnostic techniques for CD in the disease’s acute and chronic phases. The PubMed database was searched for studies published between 1990 and 2021 on CD diagnostics. Fifty-six published studies that met the criteria were analyzed and included in the meta-analysis, evaluating diagnostic accuracy through sensitivity and specificity. For Enzyme-Linked Immunosorbent Assay (ELISA), Fluorescent Antibody Technique (IFAT), Hemagglutination Test (HmT), Polymerase Chain Reaction (PCR), and Real-Time Polymerase Chain Reaction (qPCR) diagnosis methods, the sensitivity had a median of 99.0%, 78.0%, 75.0%, 76.0%, and 94.0%, respectively; while specificity presented a median of 99.0%, 99.0%, 99.0%, 98.0%, and 98.0%, respectively. This meta-analysis showed that ELISA and qPCR techniques had a higher performance compared to other methods of diagnosing CD in the chronic and acute phases, respectively. It was concluded utilizing the Area Under the Curve restricted to the false positive rates (AUCFPR), that the ELISA diagnostic test presents the highest performance in diagnosing acute and chronic CD, compared to serological and molecular tests. Future studies focusing on new CD diagnostics approaches should be targeted.

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Section: Resultsmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Accuracy of Diagnostic Tests for the Detection of Chagas Disease: A Systematic Review and Meta-Analysis

et al. 2022

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“…Thirty-four studies were selected for the ELISA technique: [35–68] in which a total of 6,054 subjects were studied. Sensitivity ranged from 78.0% to 100%, with a median of 99.0%, CI 95% (94, 100), while the test for equality of sensitivities presented a χ 2 = 657.24, df = 33, p-value = 2e-16.…”

Section: Resultsmentioning

confidence: 99%

Accuracy of the diagnostic tests for the detection of Chagas disease: a systematic review and meta-analysis

Candia-Puma

Machaca-Luque

Roque-Pumahuanca

et al. 2022

Preprint

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The present systematic review and meta-analysis about the accuracy of diagnostic tests aim to describe the findings of literature over the last thirty years for the diagnosis of Chagas disease (CD). This work aimed to determine the accuracy of diagnostic techniques for CD in the disease's acute and chronic phases. The PubMed database was searched for studies published between 1990 and 2021 on CD diagnostic. Fifty-six published studies that met the criteria were analyzed and included in the meta-analysis, evaluating diagnostic accuracy through sensitivity and specificity. For Enzyme-Linked Immunosorbent Assay (ELISA), Fluorescent Antibody Technique (IFAT), Hemagglutination Test (HmT), Polymerase Chain Reaction (PCR) and (Real-Time Polymerase Chain Reaction qPCR diagnosis methods, the sensitivity had a median of 99.0%, 78.0%, 75.0%, 76.0% and 94.0%, respectively; while specificity presented a median of 99.0%, 99.0%, 99.0%, 98.0% and 98.0%, respectively. This meta-analysis showed that ELISA and qPCR techniques had a higher performance compared to other methods of diagnosing CD in the chronic and acute phases, respectively. It was concluded by means of the AUC restricted to the false positive rates, that the ELISA diagnostic test presents the highest performance in diagnosing acute and chronic CD, compared to serological and molecular tests. Future studies focusing on new CD diagnostics approaches should be targeted.

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“…Previous data of our group evidenced the utility of TcTASV-C and TcTASV-A proteins in diagnostic tests. Despite the best performance of serology included both TcTASV-A and C subfamilies, TcTASV-A was more sensitive than TcTASV-C to detect human infections at early chronic phase (young adults and children) as well as dogs without evidence of circulating parasites 12,13 , suggesting its expression in chronic infections. TcTASV-A is one of the two major TcTASV subfamilies, has no orthologues in other organisms (from mammals to trypanosomatids) and it is also conserved in all T. cruzi lineages 8,9 .…”

Section: Selection Of a Novel Vaccine Antigenmentioning

confidence: 96%

“…All families are expressed in trypomastigotes, but there are differences between the subcellular location and additional expression in other parasite stages 8,9 . In particular, TcTASV-A and TcTASV-C are the most abundant subfamilies, they present the highest levels of expression and both are in contact with the host immune system in vivo [9][10][11][12][13] . The TcTASV-C subfamily (~15 members) is solely expressed in trypomastigotes, located at the parasite surface, and secreted both freely and contained in extracellular vesicles 10 .…”

Section: Introductionmentioning

confidence: 99%

Vaccine against Trypanosoma cruzi infection using the Parasite Antigen TcTASV Displayed at Baculovirus Capsid

Tekiel

Masip

Caeiro

et al. 2023

Preprint

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Chagas' is a neglected disease caused by the eukaryotic kinetoplastid parasite Trypanosoma cruzi. Currently there are about 8 million infected people worldwide, most of them at the chronic phase of the disease, which involves cardiac, digestive or neurologic manifestations. There is an urgent need for a vaccine because treatments are only effective at the initial phase of the infection, which is generally underdiagnosed. Selection and combination of antigens, adjuvants, and delivery platforms for vaccine formulations should be designed to trigger mixed humoral and cellular immune responses, considering that T. cruzi has a complex life cycle with both intracellular and bloodstream circulating parasite stages in the vertebrate host. Here we report the effectiveness of the vaccination with a T. cruzi-specific protein family (TcTASV), employing both recombinant proteins with aluminum hydroxide and recombinant baculovirus displaying a TcTASV antigen at the capsid. Vaccination stimulated immunological responses with production of lytic antibodies and antigen-specific CD4+ and CD8+ IFNɣ secreting lymphocytes. More than 90% of vaccinated animals survived after lethal challenges with T. cruzi while all control mice died before 30 days post infection. Vaccination also induced a strong decrease of chronic tissue parasitism, and generated immunological memory that allowed vaccinated and infected animals to control both the reactivation of the infection after immunosuppression as well as a second challenge with T. cruzi. Interestingly, inoculation with wild type baculovirus trained the immune system to partially protect mice against T. cruzi. In brief, we demonstrated, for the first time, that the combination of the baculovirus platform and the TcTASV family provides effective protection against Trypanosoma cruzi, being a promising vaccine for Chagas’ disease.

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TcTASV Antigens of Trypanosoma cruzi: Utility for Diagnosis and High Accuracy as Biomarkers of Treatment Efficacy in Pediatric Patients

Cited by 7 publications

References 18 publications

Accuracy of Diagnostic Tests for the Detection of Chagas Disease: A Systematic Review and Meta-Analysis

Accuracy of Diagnostic Tests for the Detection of Chagas Disease: A Systematic Review and Meta-Analysis

Accuracy of the diagnostic tests for the detection of Chagas disease: a systematic review and meta-analysis

Vaccine against Trypanosoma cruzi infection using the Parasite Antigen TcTASV Displayed at Baculovirus Capsid

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