2014
DOI: 10.1186/s12964-014-0050-1
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Abstract: BackgroundIn the last decade, reactive oxygen species (ROS) production has been shown to occur upon T-cell receptor (TCR) stimulation and to affect TCR-mediated signalling. However, the exact reactive species that are produced, how ROS are generated and their requirement for T-cell activation, proliferation or cytokine production remain unclear, especially in the case of primary human T cells. Moreover, several groups have questioned that ROS are produced upon TCR stimulation.ResultsTo shed some light onto thi… Show more

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Cited by 21 publications
(12 citation statements)
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References 32 publications
(43 reference statements)
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“…Additionally, in non-single cell studies the generation of ROS from contaminating phagocytes present in T-cell preparations isolated from lymphoid tissues may lead to artifacts. In fact, we have found a significant release of O 2 ·-upon stimulation of T-cell suspensions (whose purity more than 96%) with isotype controls (Belikov et al, 2014). It is believed that this non-TCR-mediated O 2 ·-originates upon stimulation of Fc receptors on monocytes contaminating purified T-cell suspensions Belikov et al, 2014).…”
Section: The Ws Of Ros Production In T Cells: When Where What and Whymentioning
confidence: 79%
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“…Additionally, in non-single cell studies the generation of ROS from contaminating phagocytes present in T-cell preparations isolated from lymphoid tissues may lead to artifacts. In fact, we have found a significant release of O 2 ·-upon stimulation of T-cell suspensions (whose purity more than 96%) with isotype controls (Belikov et al, 2014). It is believed that this non-TCR-mediated O 2 ·-originates upon stimulation of Fc receptors on monocytes contaminating purified T-cell suspensions Belikov et al, 2014).…”
Section: The Ws Of Ros Production In T Cells: When Where What and Whymentioning
confidence: 79%
“…The importance of the intracellular GSH pool in T-cell activation has been also shown using inhibitors of GSH synthesis, which reduced CD3-mediated proliferation and IL-2 production (Gmunder et al, 1990;Suthanthiran et al, 1990;Smyth, 1991;Hehner et al, 2000). More recently, it has been shown that natural antioxidants such as SOD, catalase, and ascorbate do not have any effects on the proliferation of human primary T cells upon CD3 × CD28 stimulation (Belikov et al, 2014). Thus, after three decades of intense scrutiny, it is still controversial whether ROS support or suppress T-cell functions.…”
Section: T-cell Activation Is Sensitive To Oxidationmentioning
confidence: 98%
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