TBHQ alleviates pyroptosis and necroptosis in chicken alveolar epithelial cells induced by fine particulate matter from broiler houses

Xi, Mengxue; Shen, Dan; Dai, Pengyuan; Han, Guofeng; Li, Chunmei

doi:10.1016/j.psj.2021.101593

Cited by 8 publications

(6 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 a). Different studies have shown that cellular exposure to PM 10 decreased concentration- and time-dependent cell viability 32 , 33 . However, this is not consistent with the results of our investigation.…”

Section: Discussionmentioning

confidence: 99%

Particulate matter (PM10) induces in vitro activation of human neutrophils, and lung histopathological alterations in a mouse model

Valderrama

Ortiz-Hernández

Agraz‐Cibrián

et al. 2022

Sci Rep

View full text Add to dashboard Cite

The epidemiological association between exposure to particulate matter (PM10) and various respiratory and cardiovascular problems is well known, but the mechanisms driving these effects remain unclear. Neutrophils play an essential role in immune defense against foreign agents and also participate in the development of inflammatory responses. However, the role of these cells in the PM10 induced inflammatory response is not yet fully established. Thus, this study aims to evaluate the effect of PM10 on the neutrophil-mediated inflammatory response. For this, neutrophils from healthy adult human donors were in vitro exposed to different concentrations of PM10. The cell viability and cytotoxic activity were evaluated by MTT. LDH, propidium iodide and reactive oxygen species (ROS) were quantified by flow cytometry. Interleukin 8 (IL-8) expression, peptidyl arginine deiminase 4 (PAD4), myeloperoxidase (MPO), and neutrophil elastase (NE) expression were measured by RT-PCR. IL-8 was also quantified by ELISA. Fluorescence microscopy was used to evaluate neutrophil extracellular traps (NETs) release. The in vivo inflammatory responses were assessed in BALB/c mice exposed to PM10 by histopathology and RT-PCR. The analysis shows that PM10 exposure induced a cytotoxic effect on neutrophils, evidenced by necrosis and LDH release at high PM10 concentrations. ROS production, IL-8, MPO, NE expression, and NETs release were increased at all PM10 concentrations assessed. Neutrophil infiltration in bronchoalveolar lavage fluid (BALF), histopathological changes with inflammatory cell infiltration, and CXCL1 expression were observed in PM10-treated mice. The results suggest that lung inflammation in response to PM10 could be mediated by neutrophils activation. In this case, these cells migrate to the lungs and release pro-inflamatory mediators, including ROS, IL-8, and NETs. Thus, contributing to the exacerbation of respiratory pathologies, such as allergies, infectious and obstructive diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

Particulate matter (PM10) induces in vitro activation of human neutrophils, and lung histopathological alterations in a mouse model

Valderrama

Ortiz-Hernández

Agraz‐Cibrián

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…This may be due to the strong antioxidant activity of the CA. Indeed, Xi et al [10] found that TBHQ played an important role in attenuating PM2.5-induced pyroptosis and necroptosis in chicken primary alveolar epithelial cells by inhibiting the generation of reactive oxygen species (ROS). Liu et al [30] also found that TBHQ attenuates podocyte injury in diabetic nephropathy by inhibiting NADPH oxidase-derived ROS generation via the nuclear factor E2-related factor-2 /hemeoxygenase-1 signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

“…Antioxidant TBHQ is a highly effective antioxidant for feeds, and can prevent oxidative spoilage caused by air oxidation; protect fats, oils, fat-soluble ingredients and other natural components of feeds; and delay the adverse changes caused by the oxidation of foods [8,9]. Numerous studies have shown that the antioxidant TBHQ is essential as a feed preservative in the production of livestock and poultry feeds and can effectively protect feeds against oxidative deterioration [10,11]. In vivo, TBHQ can regulate the expression of antioxidant protein genes by activating proteins; it is often used in medicine as an activator of the nuclear transcription factor E2-related factor-2 [12,13].…”

Section: Introductionmentioning

confidence: 99%

Effects of Complex Antioxidants Added to Chicken Diet on Growth Performance, Serum Biochemical Indices, Meat Quality, and Antioxidant Capacity

Chen,

Zeng,

Zeng

et al. 2024

Animals

View full text Add to dashboard Cite

This study aimed to evaluate the effects of diets supplemented with various levels of complex antioxidants (CA) containing tertiary butylhydroquinone (TBHQ) and tea polyphenols (TP) on growth performance, meat quality of breast and leg muscles, serum biochemistry, and antioxidant capacity of serum, liver, breast meat, jejunum, and ileum in broilers. A total of 600 one-day-old Arbor Acres male broilers with similar body weights were randomly divided into three groups (10 replicates/group, 20 broilers/replicate). Birds in the three experimental groups were fed a basal diet with CA at 0, 300, and 500 mg/kg. The results showed that supplementing with 300 mg/kg CA significantly increased (p < 0.05) 42 d BW and 22–42 d ADG, and markedly decreased (p < 0.05) 22–42 d F: G ratio in comparison to the control group. Birds fed a diet with 300 mg/kg CA had a higher (p < 0.05) pH of chicken meat at 24 h and 48 h post mortem and lower (p < 0.05) yellowness values (b*) of chicken meat at 45 min and 24 h post mortem, along with a lower (p < 0.05) cooking loss. Supplementing with 300 mg/kg CA significantly increased (p < 0.05) serum and liver T-SOD activity, serum T-AOC level, as well as jejunual GST activity, and significantly decreased (p < 0.05) liver MDA content when compared with the control group. These results indicate that diet supplementation with 300 mg/kg CA containing TBHQ and TP could improve growth performance and meat quality by increasing the antioxidant capacity of broilers.

show abstract

“…Moreover, LPS evidently increased the expression of immunofluorescent protein of NLRP3 in the IPEC‐J2 cells. Previous studies have demonstrated that TBHQ could alleviate pyroptosis induced by fine particulate matter in chicken alveolar epithelial cells and prevent neuroinflammation through inhibition of NLRP3 and its downstream proinflammatory factors in mouse (Bian et al, 2022; Xi et al, 2022). Interestingly, our results found that pretreatment with TBHQ effectively decreased the mRNA level and protein expression related to pyroptosis in LPS‐induced injury of IPEC‐J2 cells, moreover, it also decreased the expression of immunofluorescent protein of NLRP3 in LPS‐induced injury of IPEC‐J2 cells.…”

Section: Discussionmentioning

confidence: 99%

Tert‐butylhydroquinone attenuates LPS‐induced pyroptosis of IPEC‐J2 cells via downregulating HMGB1/TLR4/NF‐κB axis

Qiu,

Zhang,

Pang

et al. 2023

Animal Physiology Nutrition

View full text Add to dashboard Cite

Inflammatory response induced by biological stress usually occurs in weaning piglets, it reduces the production performance of piglets and even causes death. Tert‐butylhydroquinone (TBHQ) is a food additive that has the effect of anti‐inflammation and anti‐oxidation. However, there are few reports related to the protective mechanisms of TBHQ on lipopolysaccharide (LPS) induced injury in intestinal porcine epithelial (IPEC‐J2) cells. Quantitative real‐time polymerase chain reaction and western blot analysis, respectively, detected the mRNA levels and protein expressions related to pyroptosis, tight junction (TJ) protein and high‐mobility group box 1/toll‐like receptor 4/nuclear factor kappa‐B (HMGB1/TLR4/NF‐κB) axis. Localisation and expression of NOD‐like receptor pyrin domain containing 3 (NLRP3), HMGB1 and P‐NF‐κB proteins detected by immunofluorescence. The results showed that TBHQ (12.5 and 25 μM) can increase cell activity and reduce intracellular lactate dehydrogenase (LDH) levels in a dose‐dependent manner. LPS significantly decreases cell viability and increases the LDH level. However, pretreatment with TBHQ evidently increases cell viability and decreases the LDH level of IPEC‐J2 cells. In addition, treatment with LPS decreased the mRNA level and protein expression of zonula occludens‐1, occludin and claudin‐1, and increased the mRNA level and protein expression of pyroptosis and HMGB1/TLR4/NF‐κB axis. Interestingly, pretreatment with TBHQ increased the TJ protein expressions as well as decreased the mRNA level and protein expressions of pyroptosis and HMGB1/TLR4/NF‐κB axis. Moreover, the results of immunofluorescence showed that TBHQ significantly reduced the expression of NLRP3, HMGB1 and P‐NF‐κB in LPS‐induced injury of IPEC‐J2 cells. Therefore, we come to the conclusion that TBHQ attenuates LPS‐induced pyroptosis in IPEC‐J2 cells through downregulation of the HMGB1/TLR4/NF‐κB axis, TBHQ may become a potential feed additive for preventing inflammatory diarrhoea in piglets.

show abstract

TBHQ alleviates pyroptosis and necroptosis in chicken alveolar epithelial cells induced by fine particulate matter from broiler houses

Cited by 8 publications

References 48 publications

Particulate matter (PM10) induces in vitro activation of human neutrophils, and lung histopathological alterations in a mouse model

Particulate matter (PM10) induces in vitro activation of human neutrophils, and lung histopathological alterations in a mouse model

Effects of Complex Antioxidants Added to Chicken Diet on Growth Performance, Serum Biochemical Indices, Meat Quality, and Antioxidant Capacity

Tert‐butylhydroquinone attenuates LPS‐induced pyroptosis of IPEC‐J2 cells via downregulating HMGB1/TLR4/NF‐κB axis

Contact Info

Product

Resources

About