Taxonomy, phylogeny, and geographical distribution of the little-known Helicoprorodon multinucleatum Dragesco, 1960 (Ciliophora, Haptorida) and key to species within the genus

Jiang, Limin; Wang, Congcong; Zhuang, Wenbao; Li, Song; Hu, Xiaozhong

doi:10.1016/j.ejop.2021.125769

Cited by 18 publications

(9 citation statements)

References 27 publications

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“…The phylogenetic tree, based on the 18S rRNA gene, reveals unstable internal relationships and numerous weakly supported nodes within the order Spathidiida, consistent with previous reports (Chi, Wang, et al., 2021; Huang et al., 2018; Jang et al., 2017, 2022; Jiang et al., 2021; Pomahač et al., 2023; Rajter et al., 2019; Rajter & Vďačný, 2016, 2017; Vďačný et al., 2011, 2014; Yıldız, 2018). Interestingly, although Actinobolina and Papillorhabdos differ in all key features such as general appearance (ellipsoidal with numerous tentacles vs. cylindrical), extrusomes (stored in the tentacles vs. anchored to the cortex), circumoral kinety (present vs. absent), composition of somatic kineties (kinetosome clusters vs. monokinetids), and dorsal brush rows (1 vs. 4), they have an unexpectedly and fully supported phylogenetic relationship in the 18S rRNA gene tree.…”

Section: Discussionsupporting

confidence: 90%

Highly divergent morphology but a close molecular phylogenetic relationship between two little‐known ciliate genera Actinobolina and Papillorhabdos (Protozoa: Ciliophora: Litostomatea) with description of two new species

Chi,

Tang,

Lei

et al. 2023

J Eukaryotic Microbiology

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Free‐living litostomatean ciliates, prominent microeukaryote predators commonly encountered in freshwater and marine habitats, play vital roles in maintaining energy flow and nutrient cycles. Nevertheless, understanding their biodiversity and phylogenetic relationships remains challenging due to insufficient morphological information and molecular data. As a new contribution to this group, three haptorian ciliates, including two new species (Actinobolina bivacuolata sp. nov. and Papillorhabdos foissneri sp. nov.) and the insufficiently described type species, Actinobolina radians, were isolated from wetlands around Lake Weishan, China and investigated by a combination of living morphology, stained preparations, and 18S rRNA gene sequence data. An illustrated key of the valid species within the two genera is provided. In addition, we reveal the phylogenetic positions of these two genera for the first time. Although they differ in all key morphologic characters such as general appearance (ellipsoidal with numerous tentacles vs. cylindrical), extrusomes (stored in tentacles vs. anchored to pellicle), circumoral kinety (present vs. absent), composition of somatic kineties (kinetosome clusters vs. monokinetids), and number of dorsal brush rows (1 vs. 4), they both cluster in a fully supported clade in the phylogenetic tree, which indicates that the biodiversity and additional molecular markers of this group need further exploration.

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Section: Discussionsupporting

confidence: 90%

Highly divergent morphology but a close molecular phylogenetic relationship between two little‐known ciliate genera Actinobolina and Papillorhabdos (Protozoa: Ciliophora: Litostomatea) with description of two new species

Chi,

Tang,

Lei

et al. 2023

J Eukaryotic Microbiology

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“…To minimize the errors caused by PCR, Q5 Hot Start High-Fidelity 2 × Master Mix (New England BioLabs, USA) was used as DNA polymerase. The PCR parameters were utilized according to Jiang et al ( 2021 ). After amplification, PCR products were sequenced bidirectionally by the Tsingke Biological Technology Company (Qingdao, China).…”

Section: Methodsmentioning

confidence: 99%

Integrative studies on the taxonomy and molecular phylogeny of four new Pleuronema species (Protozoa, Ciliophora, Scuticociliatia)

Liu

Zhang

et al. 2022

Mar Life Sci Technol

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The ciliate genus Pleuronema comprises approximately 30 nominal species and has been reported in freshwater, brackish water, and marine habitats. Nevertheless, recent studies have indicated that there might be a large undiscovered species diversity. In the present work, four new Pleuronema species, namely P. foissneri sp. nov., P. parasmalli sp. nov., P. parasalmastra sp. nov., and P. paraorientale sp. nov., collected from Shenzhen, southern China, was investigated using taxonomic methods. The diagnosis, description, comparisons with morphologically related species and detailed morphometric data are supplied for each. The small subunit ribosomal RNA (SSU rRNA) gene of the four new species is sequenced and their molecular phylogeny is analyzed. The SSU rRNA gene tree shows that Pleuronema is polyphyletic comprising several separate clades. All four new species cluster consistently with P. orientale KF206429, P. puytoraci KF840520 and P. setigerum FJ848874 within the core Pleuronematidae + Peniculistomatidae clade. Phylogenies of Pleuronematidae-related taxa are also discussed.

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“…Q5 Hot Start high fidelity DNA polymerase (NEB, Ipswich, MA, USA) was used to amplify the SSU rRNA gene using primers Euk-A (5 -AAYCTGGTTGATYYTGCCAG-3 ) and Euk-B (5 -CYGCAGGTTCACCTACRG-3 ), resulting in a near-complete SSU rDNA fragment (Medlin et al, 1988). Cycling parameters of PCR amplification were as follows: one cycle of initial denaturation at 98 • C for 30 s, followed by 18 cycles of amplification (98 • C, 10 s; 69 • -51 • C touchdown, 30 s; 72 • C, 1 min), and another 18 cycles (98 • C, 10 s; 51 • C, 30 s; 72 • C, 1 min), with a final extension of 72 • C for 5 min (Jiang et al, 2021). The PCR products were sequenced directly in both directions by four reactions (18SF, 18SR, 900F, and 900R) by TSINGKE (Qingdao, China).…”

Section: Dna Extraction Pcr Amplification and Sequencingmentioning

confidence: 99%

Taxonomy and Molecular Phylogeny of Two New Species of Prostomatean Ciliates With Establishment of Foissnerophrys gen. n. (Alveolata, Ciliophora)

et al. 2021

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Prostomatean ciliates play important roles in the flow of material and energy in aquatic microbial food webs, and thus have attracted wide attention for over a century. Their taxonomy and systematics are, however, still poorly understood because of their relatively few taxonomically informative morphological characters. In this study, two new prostomateans, Lagynus binucleatus sp. n. and Foissnerophrys alveolata gen. n., sp. n., collected from a freshwater pool and the intertidal zone of a sandy beach, respectively, in Qingdao, China, are investigated using living observation, protargol staining, and SSU rRNA gene sequencing methods. The genus Lagynus is redefined, and the new species L. binucleatus sp. n. is established based on significant morphological differences with similar forms. Furthermore, a new genus, Foissnerophrys gen. n., is established based on a combination of morphological and molecular data with F. alveaolata sp. n. the type species by monotypy. The identities of intracellular prokaryotes of these two new species are discussed based on fluorescence in situ hybridization (FISH) data and newly obtained 16S rRNA gene sequences.

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Taxonomy, phylogeny, and geographical distribution of the little-known Helicoprorodon multinucleatum Dragesco, 1960 (Ciliophora, Haptorida) and key to species within the genus

Cited by 18 publications

References 27 publications

Highly divergent morphology but a close molecular phylogenetic relationship between two little‐known ciliate genera Actinobolina and Papillorhabdos (Protozoa: Ciliophora: Litostomatea) with description of two new species

Highly divergent morphology but a close molecular phylogenetic relationship between two little‐known ciliate genera Actinobolina and Papillorhabdos (Protozoa: Ciliophora: Litostomatea) with description of two new species

Integrative studies on the taxonomy and molecular phylogeny of four new Pleuronema species (Protozoa, Ciliophora, Scuticociliatia)

Taxonomy and Molecular Phylogeny of Two New Species of Prostomatean Ciliates With Establishment of Foissnerophrys gen. n. (Alveolata, Ciliophora)

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