Taxonomic characteristic of the strain ETH 7437 producing granaticin

Řičicová, A.; Řeháček, Z.

doi:10.1007/bf02909624

Cited by 12 publications

(1 citation statement)

References 13 publications

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“…Streptomyces granaticolor ETH 7437, a wild-type strain [15] was used throughout this study and was grown in MK medium (1 % Bactopepton, 0.4 % yeast extract, 1% glycerol, pH 7.2). E. coli XL-1Blue MRF′ (Stratagene) was used as a host strain in most DNA manipulations.…”

Section: Methodsmentioning

confidence: 99%

Characterisation of two putative protein Ser/Thr kinases from actinomycete Streptomyces granaticolor both endowed with different properties

Vomastek

Nádvorník

Janeček

et al. 1998

European Journal of Biochemistry

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The structural genes, pkg4 and pkg3, encoding two putative protein serine/threonine kinases in Streptomyces granaticolor, have been cloned and sequenced. The genes were isolated after screening genomic sublibraries with specific probes obtained by PCR amplification of chromosomal DNA using degenerate primers which correspond to amino acid sequences highly conserved in eukaryotic protein Ser/Thr kinases. The sequences of these genes predict polypeptide chains of 761 and 780 amino acids for Pkg4 and Pkg3, respectively. The genes are separated by only 2 bp and therefore probably constitute an operon. pkg4, which is positioned upstream of pkg3, contains a UUA Leu codon suggesting a developmental-dependent mode of expression. The amino-terminal half of both proteins clearly shares similarities with the family of protein Ser/Thr kinases. Both proteins studied also possess a region rich in Pro and Ala residues and a repeating motif of 11 amino acid residues, the function of which is unknown, in the carboxyterminal domain. Expression of pkg4 in Escherichia coli gave rise to two different forms : a soluble protein autophosphorylated at threonine residues and an insoluble form phosphorylated at threonine and serine residues. In contrast, when pkg3 was expressed in E. coli, no autophosphorylation was detected either in vivo or in vitro.

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Section: Methodsmentioning

confidence: 99%

Characterisation of two putative protein Ser/Thr kinases from actinomycete Streptomyces granaticolor both endowed with different properties

Vomastek

Nádvorník

Janeček

et al. 1998

European Journal of Biochemistry

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Dependence of the mycelial growth pattern on the individually regulated cell cycle in Streptomyces granaticolor

Kretschmer

1982

J of Basic Microbiology

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The growth behaviour of Streptomyces granaticolor E T H 7437 was studied by the microculture technique. The kinetics of growth and branching were recorded and, since elongation was found t o be restricted to apical elongation sites (e-sites), the rate of elongation per site (a) was determined as well. The mycelia grew exponentially. Initially the growth was dependent on a of the germ tube, but after the start of branching, growth paralleled the exponential increase of the number of branches while a attained a constant average value.Further, for liquid grown mycelia showing about the same growth kinetics the cellular structure was determined after cell wall staining. Three types of cells could be distinguished: apical branchless cells (20y0), non-apical branchless cells (20%) and non-apical cells with one branch each (60%). Since both the apical and the branched cells possessed an e-site, 80% of the cells must have been growing a t the time of sampling.Combining detailed data obtained from both the alive and the stained mycelia a model was elaborated, which may reflect the events taking place on the cellular level during mycelial growth. The model is based on the assumption that each cell behaves as a n independent unity with respect to its cell cycle. But, in contrast to the behaviour of single cell bacteria, in mycelia the two daughter cells formed upon division are neither equivalent nor uniform. Here, the sister cells differ in length, shape and posssession of a n e-site. Only one of the daughter cells receives the e-site of the mother cell, while the other starts its own cell cycle by generating a new e-site a t the cylindrical part of its envelope. Regarding the length of sister cells the degree of heterogeneity increases with the age of the corresponding region of the mycelium, and eventually some cells lose the ability t o generate an e-site, i. e. to grow.With this model the kinetic and structural peculiarities of the mycelial growth of Streptomyces granaticolor can be explained.Studying the growth behaviour of some unrelated actinomycetes strains ( SCHUH- MANN and BERGTER 1976, KRETSCHMER 1978,1981 it has become evident that niycelial growth underlies common rules. Unlimited mycelial growth is characterized by two features: exponential increase of the total length parallel to the number of branches, and elongation being restricted to elongation sites (e-sites) (BEGG and DONACHIE 1977) situated at each hyphal tip.The exponential increase of mycelial length ( SCHUHMANN and BERGTER 1976, KRETSCHMER 1978, 1981) resp. of the biomass (HILLIGER and RIESENBERG 1976)indicates that the cytoplasm is exponentially synthesized equally throughout the whole mycelium. Indeed, with Streptomyces hygroscopicus growing in a chemostat it was demonstrated autoradiographically that protein synthesis is not restricted to the hyphal tips, and that the portion of mycelial regions with high synthetic activity rises with increasing growth rate (RIESENBERG and BERGTER 1979). Also, pulse labelling the D N A of s. hygroscopicus, s. gr...

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An antibiotic‐overproducing mutant of Streptomyces granaticolor with impaired differentiation

Šťastná

Блумауерова

Janda

et al. 1987

J. Basic Microbiol.

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A thy- tetracycline-resistant mutant of Streptomyces granaticolor was prepared by mutagenesis of the parental strain ETH 7437. The mutant exhibits a different morphology and an overproduction of granaticins. The ability to form an aerial mycelium and spores has been lost. The mutant cells have a round or an atypical shape and a thick cell wall, the membraneous system is enlarged by numerous mesosomes. Division septa are formed rarely. The mutant is more sensitive to both low and high temperature than the parental strain. The altered features are stably maintained for many generations. Ribosomal proteins of the mutant do not differ substantially from those of the original strain indicating that the mutant phenotype is not due to an alteration at the translational level.

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Taxonomic characteristic of the strain ETH 7437 producing granaticin

Cited by 12 publications

References 13 publications

Characterisation of two putative protein Ser/Thr kinases from actinomycete Streptomyces granaticolor both endowed with different properties

Characterisation of two putative protein Ser/Thr kinases from actinomycete Streptomyces granaticolor both endowed with different properties

Dependence of the mycelial growth pattern on the individually regulated cell cycle in Streptomyces granaticolor

An antibiotic‐overproducing mutant of Streptomyces granaticolor with impaired differentiation

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