Taxonomic Application of Crossed Immunoelectrophoresis

Høiby, Niels; Collins, Michael T.; Espersen, Frank; Hertz, J. B.; Hoff, Gerdi E.; Schiøtz, Peter Oluf

doi:10.1099/00207713-37-3-229

Cited by 30 publications

(36 citation statements)

References 37 publications

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“…Due to taxonomic relationship between the species resulting in UTI, some of these antibodies cross-react with UTI bacteria other than the oending pathogen. 15 For calculations it was therefore necessary to compensate by using a multiplicity factor for the species with low cross-reactivity. 15 Urine cultures were performed at regular follow up visits every year or every second year.…”

Section: Methodsmentioning

confidence: 99%

“…15 For calculations it was therefore necessary to compensate by using a multiplicity factor for the species with low cross-reactivity. 15 Urine cultures were performed at regular follow up visits every year or every second year. Some of the patients have in addition delivered urine for culturing when they had speci®c symtpoms of UTI.…”

Section: Methodsmentioning

confidence: 99%

“…14 These ®ndings have led to the hypothesis that infections, in which the defense mechanisms fail to eradicate the bacteria, may result in continuous stimulation of the immune system with a pronounced antibody response, resulting in formation of immune complex mediated in¯ammation and secondary tissue damage. 12,13,15,16 We hypothesized that recurrent UTI would also induce precipitating antibodies against the oending pathogens and that the level of the antibody response may be correlated to the tissue damage and thereby the impairment of the renal function.…”

Section: Introductionmentioning

confidence: 99%

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Antibodies to urinary tract pathogens in patients with spinal cord lesions

et al. 1998

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Chronic or recurrent urinary tract infection (UTI) is a signi®cant problem for patients with spinal cord lesions (SCL). UTIs are thought to be a major factor in the development of reduced renal function. To investigate the pathogenesis 151 patients with SCL were included in this study during a 7 year period. Results of intravenous pyelography and isotope renography were recorded as well as the bladder emptying methods. One to seven blood samples were obtained from each patient and tested for plasma creatinine, and the presence of precipitating antibodies against Escherichia coli, Klebsiella pneumonia, Klebsiella ozaenae, Proteus mirabilis, Enterococcus faecalis and Pseudomonas aeruginosa. We found signi®cant correlation between duration of SCL and precipitating antibodies against urinary tract pathogens (PAU) (r=0.23, P50.005), between plasma creatinine and PAU in patients with spina bi®da (r=0.64, P50.01), between PAU and the number of positive urine cultures (r=0.17; P50.05) and a relation between abnormal urological ®ndings and PAU. In addition, the PAU was signi®cantly higher in patients with indwelling urethral catheters (P50.001). Thus it seems that PAU can be of prognostic value in SCL patients, and PAU might be an indicator for intensi®ed treatment of recurrent UTI.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antibodies to urinary tract pathogens in patients with spinal cord lesions

et al. 1998

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“…The results presented in Tables 2 and 3 provide additional support that proteins such as catalase, groEL, and flagellin are strongly immunogenic but give nonspecific cross-reactions with antibodies to antigens from other gram-negative bacteria and, more particularly, Campylobacter jejuni (10,12,17,21,26). Catalase, groEL, and flagellin will therefore be of poor utility for the envisaged predictive test.…”

Section: Vol 40 2002 Novel H Pylori Antigens 549mentioning

confidence: 88%

Novel Antigens of Helicobacter pylori Correspond to Ulcer-Related Antibody Pattern of Sera from Infected Patients

Atanassov¹,

Pezennec²,

d’Alayer

et al. 2002

J Clin Microbiol

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Recently, we reported that the patterns of antibodies to Helicobacter pylori protein antigens in serum may be useful for screening patients at high risk for ulcers ( Serological tests and assays for the diagnosis of Helicobacter pylori infection are included among the noninvasive methods recommended by the European Helicobacter pylori Study Group (7). Evaluation of the humoral immune response to H. pylori antigens by immunoblotting is a valuable alternative and complement to the more routinely used enzyme-linked immunosorbent assay (ELISA) tests (8,13,14,19,25). Immunoblotting appears to be sometimes more sensitive and useful for detecting low-abundance antibodies and antibodies directed toward nonconformational epitopes of immunodominant antigens. By this method, we recently reported that several antigens of H. pylori are preferentially recognized by the serum antibodies of adult French patients with gastroduodenal ulcers (GDU) that had been infected by this gastric pathogen. We therefore postulated that the five antigens corresponding to this antibody pattern could be useful for differentiating patients at high risk of GDU from patients with nonulcer dyspepsias (NUD) (2). Under the conditions of an immunoblot assay, two proteins of this antigenic profile were identified as CagA (125 kDa) and VacA (87 kDa), while the three other proteins were assigned only as immunoreactive bands with approximate molecular masses of 54, 42, and 35 kDa (denoted, respectively, p54, p42, and p35). Knowledge of the exact primary structure of p54, p42, and p35 is thus an essential prerequisite for preparing and further analyzing the antigenic preparation designed for the development of a test predicting the clinical outcome of the H. pylori infection.Our initial aim was to purify the three unidentified antigens in order to determine their amino acid sequences. After optimizing the electrophoretic separation of these antigens, we differentiated three antigens within the zone of p54. This prompted us to reinvestigate all members of antigenic profile by an immunoblot assay by using panels of sera from H. pyloripositive patients with GDU and NUD, as well as with the control sera of H. pylori-negative healthy volunteers. MATERIALS AND METHODSPatients and patient sera. A total of 115 individual sera were collected in the university hospitals of Poitiers (southwest), Nancy (east), and Brest (northwest). These cities represent three geographical locations in France situated at a distance of more than 700 km from one another. Eighty-five sera were obtained from patients attending the departments of hepato-gastroenterology from 1995 to 2000. All patients underwent upper gastroduodenal endoscopy with multiple antral and fundic biopsies that were processed for histology. These subjects had received neither antimicrobial nor antiacid therapies during the previous 3 months. As determined by in-house ELISA (29), all patients were seropositive for H. pylori. Of the 85 patients, 55 (43 males and 12 females) had GDU, and 30 (17 males and 13 females) ...

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“…The t test was used for pairwise comparison of mean MC values. A comprehensive review of the taxonomic application of crossed immunoelectrophoresis has recently been published (31).…”

Section: Methodsmentioning

confidence: 99%

Antigenic Heterogeneity Among Legionella, Fluoribacter, and Tatlockia Species Analyzed by Crossed Immunoelectrophoresis

Collins

Bangsborg

Høiby

1987

International Journal of Systematic Bacteriology

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Crossed immunoelectrophoresis (XIE) reference systems were established for Fluoribacter (Legionella) (containing Fluoribacter bozemanae, Fluoribacter dumofii, and Fluoribacter gormanii) and for Tatlockia (Legionella) micdadei. The Fluoribacter and Tatlockia XIE reference systems contained 54 and 72 anodemigrating antigens, respectively. These two systems, together with the previously described polyvalent Legionella pneumophila (serogroups 1 through 6) XIE reference system, were used to study the crossreactivities of antigens from organisms comprising the three proposed genera in the family Legionellaceae. Antigenic homology was expressed as the matching coefficient (MC), the ratio of the number of cross-reactive antigens to the total number of antigens. The MCs for individual L. pneumophila serogroups when the polyvalent L. pneumophila antibody was used were 0.98 f 0.05, which was significantly higher than the MCs determined by using Fluoribacter or Tatlockia antibodies (0.50 f 0.13) (P < 0.001). The MCs for the three species of Fluoribacter when polyvalent Fluoribacter antibody was used were 0.93 & 0.10, which was also significantly higher than the MCs when heterologous antibodies were used (0.40 f 0.04) (P < 0.001). The MCs for T . micdadei with the two heterologous antibody preparations were similar to each other (0.32 and 0.46) and to all other heterologous MCs among members of the Legionellaceae. The MCs for organisms representing three other families of bacteria were 0.16 f 0.04 in all three XIE reference systems and were significantly lower than the MCs among members of the Legionellaceae (P < 0.001). When a priori criteria for MC interpretation established in previous serotaxonomic studies of other bacterial species by XIE were used, our results from studies on the antigenic relationships among Legionella, Fluoribacter, and Tatlockia supported the proposal that there are multiple genera in the family Legionellaceae.Antigenic analysis of proteins or peptides has been used to study phylogenetic relationships among both procaryotes and eucaryotes (11, 18,44). In most studies, purified enzymes and monospecific polyclonal antisera have been used. These studies have demonstrated that protein antigens do not cross-react unless they are homologous and isofunctional and have a common phylogenetic origin (11,(42)(43)(44)52), that the degree of cross-reaction is detectable until 30 to 40% of the amino acid sequence is substituted (43,44), and that there is a linear correlation between immunological distance and percentage of deoxyribonucleic acid-ribonucleic acid homology down to homology values of 45 to 50% (4). We previously reported one-way cross-reaction studies in which a Legionella pneumophila serogroup 1 crossed immunoelectrophoresis reference system was used (13). Our findings were similar to those of Jolly and Kenny (33), who, also by using crossed immunoelectrophoresis, showed that there was a high degree of antigenic homogeneity among L . pneumophila serogroups 1 through 4 (27 of 31 antigens in common) a...

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Taxonomic Application of Crossed Immunoelectrophoresis

Cited by 30 publications

References 37 publications

Antibodies to urinary tract pathogens in patients with spinal cord lesions

Antibodies to urinary tract pathogens in patients with spinal cord lesions

Novel Antigens of Helicobacter pylori Correspond to Ulcer-Related Antibody Pattern of Sera from Infected Patients

Antigenic Heterogeneity Among Legionella, Fluoribacter, and Tatlockia Species Analyzed by Crossed Immunoelectrophoresis

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