Taxa-specific changes in soil microbial community composition induced by pyrogenic carbon amendments

Khodadad, Christina L.; Zimmerman, Andrew R.; Green, Stefan J.; Uthandi, Sivakumar; Foster, Jamie S.

doi:10.1016/j.soilbio.2010.11.005

Cited by 338 publications

(180 citation statements)

References 27 publications

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“…Gene copy numbers per g dry soil were calculated according to Behrens et al (2008). Total bacterial cell numbers per g dry soil were calculated from the qPCR 16S rRNA gene copy numbers considering the average bacterial rRNA operon number (4.2) as derived from the Ribosomal RNA Operon Copy Number Database (http://rrndb.mmg.msu.edu/ index.php) (Klappenbach et al, 2001). Transcript copy numbers were normalized to nanogram RNA.…”

Section: Molecular Biological Analysesmentioning

confidence: 99%

“…Several studies have documented that biochar induces shifts in the microbial community composition (Rondon et al, 2007;Steinbeiss et al, 2009;Anderson et al, 2011;Khodadad et al, 2011;Ducey et al, 2013), whereas other studies described that the addition of biochar to soils does affect soil N 2 O emissions (Yanai et al, 2007;Singh et al, 2010b;Taghizadeh-Toosi et al, 2011;Felber et al, 2012;Cayuela et al, 2013). However, a potential link between the observed shifts in microbial community composition and the decreased soil N 2 O emissions has not been subject of systematic investigation so far.…”

Section: Introductionmentioning

confidence: 99%

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Linking N2O emissions from biochar-amended soil to the structure and function of the N-cycling microbial community

Harter¹,

Krause²,

Schuettler³

et al. 2013

The ISME Journal

519

258

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Nitrous oxide (N 2 O) contributes 8% to global greenhouse gas emissions. Agricultural sources represent about 60% of anthropogenic N 2 O emissions. Most agricultural N 2 O emissions are due to increased fertilizer application. A considerable fraction of nitrogen fertilizers are converted to N 2 O by microbiological processes (that is, nitrification and denitrification). Soil amended with biochar (charcoal created by pyrolysis of biomass) has been demonstrated to increase crop yield, improve soil quality and affect greenhouse gas emissions, for example, reduce N 2 O emissions. Despite several studies on variations in the general microbial community structure due to soil biochar amendment, hitherto the specific role of the nitrogen cycling microbial community in mitigating soil N 2 O emissions has not been subject of systematic investigation. We performed a microcosm study with a water-saturated soil amended with different amounts (0%, 2% and 10% (w/w)) of high-temperature biochar. By quantifying the abundance and activity of functional marker genes of microbial nitrogen fixation (nifH), nitrification (amoA) and denitrification (nirK, nirS and nosZ) using quantitative PCR we found that biochar addition enhanced microbial nitrous oxide reduction and increased the abundance of microorganisms capable of N 2 -fixation. Soil biochar amendment increased the relative gene and transcript copy numbers of the nosZ-encoded bacterial N 2 O reductase, suggesting a mechanistic link to the observed reduction in N 2 O emissions. Our findings contribute to a better understanding of the impact of biochar on the nitrogen cycling microbial community and the consequences of soil biochar amendment for microbial nitrogen transformation processes and N 2 O emissions from soil.

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Section: Molecular Biological Analysesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Linking N2O emissions from biochar-amended soil to the structure and function of the N-cycling microbial community

Harter¹,

Krause²,

Schuettler³

et al. 2013

The ISME Journal

519

258

View full text Add to dashboard Cite

show abstract

“…Similar results have been observed in terrestrial soil communities. In experimental grassland plots exposed to 650 ppmv of CO 2 there was no detectable increase in the species richness of the communities even after five years of exposure [49], although increases in biomass have been frequently observed and may be the result of increase carbon sequestration in the soils [50][51][52]. The lack of change in the overall microbial diversity of the microbialites may also reflect the adaptive nature of these communities to environmental perturbations, including atmospheric CO 2 .…”

Section: Changes In Microbial Diversity In Response To Elevated Co 2 mentioning

confidence: 99%

Effects of Elevated Carbon Dioxide and Salinity on the Microbial Diversity in Lithifying Microbial Mats

et al. 2014

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Atmospheric levels of carbon dioxide (CO 2 ) are rising at an accelerated rate resulting in changes in the pH and carbonate chemistry of the world's oceans. However, there is uncertainty regarding the impact these changing environmental conditions have on carbonate-depositing microbial communities. Here, we examine the effects of elevated CO 2 , three times that of current atmospheric levels, on the microbial diversity associated with lithifying microbial mats. Lithifying microbial mats are complex ecosystems that facilitate the trapping and binding of sediments, and/or the precipitation of calcium carbonate into organosedimentary structures known as microbialites. To examine the impact of rising CO 2 and resulting shifts in pH on lithifying microbial mats, we constructed growth chambers that could continually manipulate and monitor the mat environment. The microbial diversity of the various treatments was compared using 16S rRNA gene pyrosequencing. The results indicated that elevated CO 2 levels during the six month exposure did not profoundly alter the microbial diversity, community structure, or carbonate precipitation in the microbial mats; however some key taxa, such as the sulfate-reducing bacteria Deltasulfobacterales, were enriched. These results suggest that some carbonate depositing ecosystems, such as the microbialites, may be more resilient to anthropogenic-induced environmental change than previously thought. OPEN ACCESSMinerals 2014, 4 146

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“…Biochar has a large specific surface area, porous microstructure, and abundant surface functional groups, which can provide favorable soil conditions for microbes (Farrell et al, 2013;Milla et al, 2013). Moreover, the amount and structural diversity of soil microbes can change after the application of biochar (Khodadad et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Effects of biochar on nutrients and the microbial community structure of tobacco-planting soils

Gao

Wang²,

Shen

et al. 2017

J. Soil Sci. Plant Nutr.

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The effect of rice straw biochar on soil nutrients was studied using a randomized block experimental design in a tobacco-planting field, and differences in soil bacterial and fungal community structures were studied using high-throughput sequencing technology. The results indicated that the soil pH, CEC, total organic carbon (TOC), inorganic carbon (IC), soil total nitrogen (total N), total potassium (total K), available phosphorus (available P), and available potassium (available K) contents were all increased with the biochar application. TOC and available P contents increased by 38.19% and 22.38%, respectively, in the T2 treatment (4500 kg . ha -1 biochar) compared to those in control (CK). But the soil dissolved organic carbon ( DOC) and available nitrogen (available N) content decreased by 13.07% and 9.89% in T2 treatments compared to the CK, respectively. Soil bacteria composition mainly consisted of Proteobacteria, Actinobacteria, and Acidobacteria, and the Proteobacteria proportion represented >50% of the bacterial community. The Actinobacteria proportion decreased, but Proteobacteria and Acidobacteria proportions increased with increasing biochar amounts. Fungi in tobaccoplanting soils were mainly Ascomycota (>75%), but this proportion decreased as the biochar amount increased.However, Zygomycota and Basidiomycota proportions increased with increasing biochar amounts. Therefore, biochar application could improve the soil nutrient status and affect microbial community structure. Rice straw biochar application promoted fungal community diversity in tobacco-planting soils.

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Taxa-specific changes in soil microbial community composition induced by pyrogenic carbon amendments

Cited by 338 publications

References 27 publications

Linking N2O emissions from biochar-amended soil to the structure and function of the N-cycling microbial community

Linking N2O emissions from biochar-amended soil to the structure and function of the N-cycling microbial community

Effects of Elevated Carbon Dioxide and Salinity on the Microbial Diversity in Lithifying Microbial Mats

Effects of biochar on nutrients and the microbial community structure of tobacco-planting soils

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