Tat-APE1/ref-1 protein inhibits TNF-α-induced endothelial cell activation

Song, Yun; Lee, Ji Young; Joo, Hee Kyoung; Kim, Hyo Shin; Lee, Sang Ki; Lee, Kwon-Ho; Cho, Chung–Hyun; Park, Jin Bong; Jeon, Byeong Hwa

doi:10.1016/j.bbrc.2008.01.037

Cited by 15 publications

(9 citation statements)

References 17 publications

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“…AP endonuclease/ Redox factor-1 (APE/Ref-1) was reported to have the ability to inhibit endothelial apoptosis and to suppress TNF-alpha induced expression of VCAM-1 [20] as well as the subsequent monocyte adhesion [34]. VCAM-1 and monocyte adhesion plays a critical role in the development of atherosclerosis.…”

Section: Discussionmentioning

confidence: 99%

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Resistance exercise training increase activation of AKT-eNOS and Ref-1 expression by FOXO-1 activation in aorta of F344 rats

Yoon

et al. 2015

J. Exerc. Nutr, Biochem.

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. Resistance exercise training increase activation of AKT-eNOS and Ref-1 expression by FOXO-1 activation in aorta of F344 rats. JENB., Vol. 19, No. 3, pp.165-171, 2015 [Purpose] This study investigated the effects of resistance exercise on the Akt-eNOS, the activation of antioxidant protein and FOXO1 in the aorta of F344 rats.[Methods] Male 7 week-old F344 rats were randomly divided into 2 groups: a climbing group (n = 6) and a sedentary group (n = 6). H&E staining and western blotting were used to analyze the rat aortas and target proteins.

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Section: Discussionmentioning

confidence: 99%

“…Protein expression was detected using an enhanced chemiluminescence kit (Amersham Pharmacia Biotech) [20]. The band intensities were quantified by densitometry using a Bio-Rad image analysis system (Quantity One, Bio-Rad, USA), and normalized against the b-actin protein.…”

Section: Western Blottingmentioning

confidence: 99%

Resistance exercise training increase activation of AKT-eNOS and Ref-1 expression by FOXO-1 activation in aorta of F344 rats

Yoon

et al. 2015

J. Exerc. Nutr, Biochem.

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“…For quantitative adhesion assay, U937 cells were fluorescently labeled with 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxy-fluorescein acetoxymethyl ester (BCECF-AM) by incubating the cells (1×10 7 cells/ml) with 1 µmol/L BCECF-AM in RPMI-1640 medium for 30 min at 37 o C and 5% CO 2 as previously described [10][11][12] . HUVECs were seeded in 24-well plates to reach confluent monolayer and pretreated with KRGE for 24 hours in EGM-2 medium.…”

Section: Monocyte Adhesion Assaymentioning

confidence: 99%

Korean Red Ginseng Extract inhibits Tumor Necrosis Factor-alpha-induced Monocyte Adhesion in the Human Endothelial Cells

2008

Journal of Ginseng Research

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Abstracts : Vascular inflammation is an important step in the development of cardiovascular disorder. Since it has not been known whether Korean red ginseng has a role to play on the vascular inflammation, we investigated the effects of Korean red ginseng extract (KRGE) on monocyte adhesion and its underlying signaling mechanism. Monocyte adhesion assay and Western blot were conducted on the human umbilical vein endothelial cells to study monocyte adhesion and the expression of adhesion molecules. Intracellular calcium was measured with Fura-2 fluorescent staining, and superoxide production was measured with lucigenin chemiluminescence in the endothelial cells. KRGE inhibits tumor necrosis factor (TNF)-alpha-induced monocyte adhesion on the endothelial cells at the range of 0.03~1 mg/ml. TNF-alpha-induced vascular cell adhesion molecule-1 and intercellular cell adhesion molecule-1 expression were inhibited by the pretreatment of KRGE in the endothelial cells. KRGE also inhibits TNF-alpha-induced intracellular calcium and the superoxide production in the endothelial cells. This study first demonstrated that KRGE inhibits TNF-alpha-induced monocyte adhesion by inhibiting the adhesion molecule expression, intracellular calcium and superoxide production in the endothelial cells. Therefore, the anti-inflammatory function of KRGE may be contributed to protecting the endothelial dysfunction in the vascular inflammatory disorders.

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“…Ref-1 has also been shown to suppress intracellular oxidative stress and apoptosis (Angkeow et al, 2002;Ozaki et al, 2002;Yoo et al, 2004). It was recently reported that vascular endothelial cell activation and vascular inflammation were inhibited by Ref-1 overexpression (Kim et al, 2006;Song et al, 2008;Lee et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of Ref-1 Inhibits Lead-induced Endothelial Cell Death via the Upregulation of Catalase

Lee

Kim

et al. 2009

Korean J Physiol Pharmacol

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Tat-APE1/ref-1 protein inhibits TNF-α-induced endothelial cell activation

Cited by 15 publications

References 17 publications

Resistance exercise training increase activation of AKT-eNOS and Ref-1 expression by FOXO-1 activation in aorta of F344 rats

Resistance exercise training increase activation of AKT-eNOS and Ref-1 expression by FOXO-1 activation in aorta of F344 rats

Korean Red Ginseng Extract inhibits Tumor Necrosis Factor-alpha-induced Monocyte Adhesion in the Human Endothelial Cells

Overexpression of Ref-1 Inhibits Lead-induced Endothelial Cell Death via the Upregulation of Catalase

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