Targeting of Hematopoietic Progenitor Cells with MR Contrast Agents

Daldrup-Link, Heike E.; Rudelius, Martina; Oostendorp, Robert A.J.; Settles, Marcus; Piontek, Guido; Metz, Stefan; Rosenbrock, Hilkea; Keller, Ulrich auf dem; Heinzmann, Ulrich; Rummeny, Ernst J.; Schlegel, Jürgen; Link, Thomas M.

doi:10.1148/radiol.2283020322

Cited by 195 publications

(172 citation statements)

References 25 publications

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“…In this study, we did not perform additional investigations of the cell function before and after the labeling procedure. In previous studies, we investigated the function of hematopoietic cells more extensively and did not find any impairment of the cells due to our labeling procedure (26,39). This is in accordance with data from the literature (17,20,22,25,(40)(41)(42).…”

Section: Discussionsupporting

confidence: 91%

In vivo tracking of genetically engineered, anti-HER2/neu directed natural killer cells to HER2/neu positive mammary tumors with magnetic resonance imaging

Daldrup-Link

Meier

Rudelius³

et al. 2004

Eur Radiol

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159

147

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Section: Discussionsupporting

confidence: 91%

In vivo tracking of genetically engineered, anti-HER2/neu directed natural killer cells to HER2/neu positive mammary tumors with magnetic resonance imaging

Daldrup-Link

Meier

Rudelius³

et al. 2004

Eur Radiol

Self Cite

159

147

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“…14 Here we used Effectene to transfect the MRI contrast agent Gd-DTPA into stem cells, because it is relatively more efficient and less toxic than liposome, calcium phosphate, or viral vectors for transfection into primary cells. [15][16][17] There was a possibility that phagocytosed or dead transplanted cells with Gd-DTPA were detected by the MRI. However, a previous pharmacokinetic study reported that Gd-DTPA in dead cells or interstitial spaces was washed out within 24 hours.…”

Section: Discussionmentioning

confidence: 99%

Efficient Tracking of Non-Iron-Labeled Mesenchymal Stem Cells With Serial MRI in Chronic Stroke Rats

Shyu

Chen

Lin

et al. 2007

Stroke

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Background and Purpose-Although stem cell-based treatments for neurodegenerative diseases have advanced rapidly, there is currently no clinically available method to monitor the fate of transplanted cells in the brain. Methods-To use magnetic resonance imaging for tracking transplanted stem cells in the ischemic rat brain, we used the cellular labeling substance Effectene to transfect a standard contrast agent (Gd-DTPA) into immortalized human bone marrow stromal cells. Results-The transfection efficiency of this method was up to 90%, which is substantially better than pure spontaneous endocytosis or other transfection agents. In addition, cellular uptake of Gd-DTPA in vitro was maintained for Ͼ28 days. Therefore, we could follow transplanted stem cell migration and homing into the penumbric area. Using double immunofluorescence, the transplanted cells were seen to differentiate into glial cells, neurons and vascular endothelial cells. Cortical neurochemical activity as evaluated by proton magnetic resonance spectroscopy ( 1 H-MRS) also increased considerably after immortalized human bone marrow stromal cell transplantation. Conclusion-This method of tracking immortalized human bone marrow stromal cells is highly efficient and allows for nontoxic labeling of cells.

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“…Enhanced detection of bone marrow is possible through SPIO 27,61 and possibly SSPIO, such as AMI-25 as well. 146 Bone marrow lesions are usually detected with high sensitivity, except in the complicating cases of children and 117 adults with red marrow hyperplasia.…”

Section: Cancer Imagingmentioning

confidence: 99%

“…The cells were Internalizing cell surface receptor-mediated endocytosis of SPIO is another avenue through which cell migration can be observed in vivo. 27 Bulte and coworkers utilized anti-transferrin receptor antibodies conjugated to nanoparticles in order to observe oligodendrocyte migration postneurotransplantation. 19 The SPIO did not interfere with the cells' ability to myelinate in vivo, and histological evaluation of the cells with nanoparticles revealed cell migration up to 10 mm in 14 days.…”

Section: Cell Trackingmentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

et al. 2006

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Abstract-The field of molecular imaging has recently seen rapid advances in the development of novel contrast agents and the implementation of insightful approaches to monitor biological processes non-invasively. In particular, superparamagnetic iron oxide nanoparticles (SPIO) have demonstrated their utility as an important tool for enhancing magnetic resonance contrast, allowing researchers to monitor not only anatomical changes, but physiological and molecular changes as well. Applications have ranged from detecting inflammatory diseases via the accumulation of non-targeted SPIO in infiltrating macrophages to the specific identification of cell surface markers expressed on tumors. In this article, we attempt to illustrate the broad utility of SPIO in molecular imaging, including some of the recent developments, such as the transformation of SPIO into an activatable probe termed the magnetic relaxation switch.

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Targeting of Hematopoietic Progenitor Cells with MR Contrast Agents

Abstract: Hematopoietic progenitor cells can be labeled with MR contrast agents and can be depicted with a standard 1.5-T MR imager.

Cited by 195 publications

References 25 publications

In vivo tracking of genetically engineered, anti-HER2/neu directed natural killer cells to HER2/neu positive mammary tumors with magnetic resonance imaging

In vivo tracking of genetically engineered, anti-HER2/neu directed natural killer cells to HER2/neu positive mammary tumors with magnetic resonance imaging

Efficient Tracking of Non-Iron-Labeled Mesenchymal Stem Cells With Serial MRI in Chronic Stroke Rats

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

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