Targeting graphene quantum dots to epidermal growth factor receptor for delivery of cisplatin and cellular imaging

Nasrollahi, Fatemeh; Koh, Yun Rui; Chen, Peng; Varshosaz, Jaleh; Khodadadi, Abbas Ali; Lim, Sierin

doi:10.1016/j.msec.2018.09.020

Cited by 68 publications

(43 citation statements)

References 54 publications

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“…GQDs showed high cell viability (more than 85%) on the MDA‐MB‐231 cells at concentrations lower than 100 µg mL −1 , while they exhibited similar cell viability on 3T3 cells at concentrations lower than 50 µg mL −1 (Figure S7a,b, Supporting Information). It is in agreement with other investigations, which studied cytotoxicity of GQDs on MDA‐MB231 and 3T3 cell lines . Moreover, Figure 6a revealed the proliferative effect of (Fe)AA on MDA‐MB‐231 cells which significantly decreased ( p < 0.05) by increasing the concentration of (Fe)AA.…”

Section: Resultssupporting

confidence: 91%

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Incorporation of Graphene Quantum Dots, Iron, and Doxorubicin in/on Ferritin Nanocages for Bimodal Imaging and Drug Delivery

Nasrollahi

Sana

Paramelle

et al. 2020

Advanced Therapeutics

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Graphene quantum dots (GQDs) have been emerging as next‐generation bioimaging agents because of their intrinsic strong fluorescence, photostability, aqueous stability, biocompatibility, and facile synthesis. In this work, GQDs are encapsulated in ferritin protein nanocages to develop multi‐functional nanoplatforms toward multi‐modal imaging and cancer therapy. Encapsulation of ultra‐small GQDs is expected to reduce their quick excretion from the body and increase their bioimaging efficiency. To expand the functionality of protein nanocages as multi‐modal imaging nanoprobes capable of both fluorescence and magnetic resonance imaging (MRI), GQDs and iron are encapsulated inside the core of AfFtn‐AA (an engineered ferritin nanocage derived from the archaeon Archaeoglobus fulgidus). The co‐encapsulation is achieved through an iron‐mediated, self‐assembly of ferritin dimers resulting in the formation of GQD–iron complex in the ferritin nanocages ((GQDs/Fe)AA). The (GQDs/Fe)AA shows high relaxivities in MRI and pH‐sensitive fluorescence with strong fluorescence at low pH values and on MDA‐MB‐231 cells. As an imaging agent and a drug nanocarrier, (GQDs/Fe)AA exhibits negligible cytotoxicity on the cells and a high loading capacity (35%) of doxorubicin. Taken together, the (GQDs/Fe)AA shows promising applications in cancer diagnosis and therapy as a pH‐responsive fluorophore, MRI agent, and drug nanocarrier.

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Section: Resultssupporting

confidence: 91%

“…The unique properties of graphene derivatives have made them attractive nanomaterials in drug delivery and biomaging . Among graphene derivatives, graphene quantum dots (GQDs) have minimum toxicity owing to their ultra‐small size (<10 nm) and oxygen functional groups in their structure .…”

Section: Introductionmentioning

confidence: 99%

Incorporation of Graphene Quantum Dots, Iron, and Doxorubicin in/on Ferritin Nanocages for Bimodal Imaging and Drug Delivery

Nasrollahi

Sana

Paramelle

et al. 2020

Advanced Therapeutics

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“…The reduced uptake exhibited by most of our GQD:MB combinations could be due to unique size, structural or charge properties that prevent transit across the MCF-7 cell membrane. Although some GQDs can enter cells without modification (32), many graphene quantum dots are not able to cross cancer cell membranes (33)(34)(35)(36)(37)(38). Similarly, methylene blue can also exhibit reduced membrane permeability in cancer cell lines (18,(39)(40)(41)(42).…”

Section: Cell Uptake Measurementmentioning

confidence: 99%

Anticancer Photodynamic Therapy Properties of Sulfur‐Doped Graphene Quantum Dot and Methylene Blue Preparations in MCF‐7 Breast Cancer Cell Culture

Monroe

Belekov

et al. 2019

Photochem & Photobiology

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Photodynamic therapy (PDT) is a field with many applications including chemotherapy. Graphene quantum dots (GQDs) exhibit a variety of unique properties and can be used in PDT to generate singlet oxygen that destroys pathogenic bacteria and cancer cells. The PDT agent, methylene blue (MB), like GQDs, has been successfully exploited to destroy bacteria and cancer cells by increasing reactive oxygen species generation. Recently, combinations of GQDs and MB have been shown to destroy pathogenic bacteria via increased singlet oxygen generation. Here, we performed a spectrophotometric assay to detect and measure the uptake of GQDs, MB and several GQD‐MB combinations in MCF‐7 breast cancer cells. Then, we used a cell counting method to evaluate the cytotoxicity of GQDs, MB and a 1:1 GQD:MB preparation. Singlet oxygen generation in cells was then detected and measured using singlet oxygen sensor green. The dye, H2DCFDA, was used to measure reactive oxygen species production. We found that GQD and MB uptake into MCF‐7 cells occurred, but that MB, followed by 1:1 GQD:MB, caused superior cytotoxicity and singlet oxygen and reactive oxygen species generation. Our results suggest that methylene blue's effect against MCF‐7 cells is not potentiated by GQDs, either in light or dark conditions.

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“…Graphene is an emerging nanocarrier in biomaterial science. Graphene-based nanocarrier is used in a drug delivery system because of its high aqueous solubility and surface functionalization [120]. Graphene quantum dots (GQDs) were utilized for dualfunctioning drug targeting and biomedical cellular imaging.…”

Section: Graphenementioning

confidence: 99%

“…Graphene quantum dots (GQDs) were utilized for dualfunctioning drug targeting and biomedical cellular imaging. Western blot analysis with confocal imaging explored the targeting of epidermal growth factor receptor (EGFR) overexpression in breast cancer cells [120]. The modified graphene coated silver nanocomposite-loaded cisplatin was synthesized for the enhancement of the apoptosis and autophagy against the human cervical cancer cell.…”

Section: Graphenementioning

confidence: 99%

Recent progress in nanotechnology-based novel drug delivery systems in designing of cisplatin for cancer therapy: an overview

Farooq

Aquib

Farooq

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

108

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2019) Recent progress in nanotechnology-based novel drug delivery systems in designing of cisplatin for cancer therapy: an overview, Artificial ABSTRACT Cisplatin cis-(diammine)dichloridoplatinum(II) (CDDP) is the first platinum-based complex approved by the food and drug administration (FDA) of the United States (US). Cisplatin is the first line chemotherapeutic agent used alone or combined with radiations or other anti-cancer agents for a broad range of cancers such as lung, head and neck. Aroplatin TM , Lipoplatin TM and SPI-077 are PEGylated liposomebased nano-formulations that are still under clinical trials. They have many limitations, for example, poor aqueous solubility, drug resistance and toxicities, which can be overcome by encapsulating the cisplatin in Nemours nanocarriers. The extensive literature from different electronic databases covers the different nano-delivery systems that are developed for cisplatin. This review critically emphasizes on the recent advancement, development, innovations and updated literature reported for different carrier systems for CDDP. ARTICLE HISTORY Current status of FDA-approved/under clinical trials CDDP nano-formulationsMany liposomal-based nano-formulations are at the stage of clinical trials. One of under clinical trials drugs, Lipoplatin (Regulon, Inc.), is liposome-based platinum formulation under Phase III clinical trials [11]. It contains a combination of cisplatin (9%) and lipids (91%(w/w)), including soy phosphatidylcholine (SPC-3), cholesterol, dipalmitoyl phosphatidyl glycerol (DPPG) and methoxy-PEG-distearoyl phosphatidyl

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Targeting graphene quantum dots to epidermal growth factor receptor for delivery of cisplatin and cellular imaging

Cited by 68 publications

References 54 publications

Incorporation of Graphene Quantum Dots, Iron, and Doxorubicin in/on Ferritin Nanocages for Bimodal Imaging and Drug Delivery

Incorporation of Graphene Quantum Dots, Iron, and Doxorubicin in/on Ferritin Nanocages for Bimodal Imaging and Drug Delivery

Anticancer Photodynamic Therapy Properties of Sulfur‐Doped Graphene Quantum Dot and Methylene Blue Preparations in MCF‐7 Breast Cancer Cell Culture

Recent progress in nanotechnology-based novel drug delivery systems in designing of cisplatin for cancer therapy: an overview

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