Targeting cell-type-specific, choroid-peripheral immune signaling to treat age-related macular degeneration

MMP9 Genotype and Systemic T-Cell Subsets Correlate With Structural and Functional Outcomes in Neovascular Age-Related Macular Degeneration

“…Nonetheless, the systemic immune alterations we observed are echoed by epidemiological links between AMD and systemic autoimmune diseases (e.g., higher AMD rates in Crohn's disease and lupus). 77 Moreover, our finding that peripheral T-cell profiles correlate with retinal fluid is in line with recent work by Lin et al, 78 which found altered systemic immune signaling associated with AMD severity (including nvAMD). This growing evidence supports a broader role for systemic immunity in AMD.…”

MMP9 Genotype and Systemic T-Cell Subsets Correlate With Structural and Functional Outcomes in Neovascular Age-Related Macular Degeneration

“…Nonetheless, the systemic immune alterations we observed are echoed by epidemiological links between AMD and systemic autoimmune diseases (e.g., higher AMD rates in Crohn's disease and lupus). 77 Moreover, our finding that peripheral T-cell profiles correlate with retinal fluid is in line with recent work by Lin et al, 78 which found altered systemic immune signaling associated with AMD severity (including nvAMD). This growing evidence supports a broader role for systemic immunity in AMD.…”

Elevation of Granulocyte Colony Stimulating Factor in Human AMD Donor RPE-Choroid

“…21 Cytokines signal to immune cells promoting inflammation and draw mononuclear cells to sites of tissue injury. A recent study has identified that local alterations in the extracellular matrix protein CYR61 in choroidal veins may be involved in the recruitment of circulating monocytes, 22 supporting the hypothesis that local alterations in inflammatory molecules may contribute to increased mononuclear cell infiltration and activation in AMD and thus could be responsible for the increased numbers of activated macrophages observed in the submacular region of AMD donor eyes. 4 Although there is significant evidence for a local inflammatory response in early/intermediate AMD, less is known about local alterations in inflammatory cytokines in the human RPE and choroid.…”

Type V Collagen Directs the Differentiation of iPSCs into Valvular Endothelial‐Like Cells

“…PCA and cluster analysis (Figure S9A,B) suggested that there were excellent intra-group similarities and significant differences between the two groups, which ensured the feasibility of subsequent histological analysis and the accuracy of the data. To further validate the role of Col V and assess the similarity between hVECs and iVEC-C, we performed hierarchical clustering analysis on five endothelial cell groups, including iVEC-M, iVEC-C, primary hVECs (HVEC: PRJNA552159), human aortic endothelial cells (HAEC: GSE89970), and human umbilical vein endothelial cells (HUVEC: GSE222101) [48][49][50]. The hierarchical clustering analysis and Spearman correlation analysis indicated that iVEC-C transcriptionally aligns more closely with primary hVECs than with other vascular endothelial subtypes (Figure S9C,D).…”

MMP9 Genotype and Systemic T-Cell Subsets Correlate With Structural and Functional Outcomes in Neovascular Age-Related Macular Degeneration

“…Nonetheless, the systemic immune alterations we observed are echoed by epidemiological links between AMD and systemic autoimmune diseases (e.g., higher AMD rates in Crohn's disease and lupus). 77 Moreover, our finding that peripheral T-cell profiles correlate with retinal fluid is in line with recent work by Lin et al, 78 which found altered systemic immune signaling associated with AMD severity (including nvAMD). This growing evidence supports a broader role for systemic immunity in AMD.…”

Elevation of Granulocyte Colony Stimulating Factor in Human AMD Donor RPE-Choroid

“…21 Cytokines signal to immune cells promoting inflammation and draw mononuclear cells to sites of tissue injury. A recent study has identified that local alterations in the extracellular matrix protein CYR61 in choroidal veins may be involved in the recruitment of circulating monocytes, 22 supporting the hypothesis that local alterations in inflammatory molecules may contribute to increased mononuclear cell infiltration and activation in AMD and thus could be responsible for the increased numbers of activated macrophages observed in the submacular region of AMD donor eyes. 4 Although there is significant evidence for a local inflammatory response in early/intermediate AMD, less is known about local alterations in inflammatory cytokines in the human RPE and choroid.…”

Type V Collagen Directs the Differentiation of iPSCs into Valvular Endothelial‐Like Cells

“…PCA and cluster analysis (Figure S9A,B) suggested that there were excellent intra-group similarities and significant differences between the two groups, which ensured the feasibility of subsequent histological analysis and the accuracy of the data. To further validate the role of Col V and assess the similarity between hVECs and iVEC-C, we performed hierarchical clustering analysis on five endothelial cell groups, including iVEC-M, iVEC-C, primary hVECs (HVEC: PRJNA552159), human aortic endothelial cells (HAEC: GSE89970), and human umbilical vein endothelial cells (HUVEC: GSE222101) [48][49][50]. The hierarchical clustering analysis and Spearman correlation analysis indicated that iVEC-C transcriptionally aligns more closely with primary hVECs than with other vascular endothelial subtypes (Figure S9C,D).…”

MMP9 Genotype and Systemic T-Cell Subsets Correlate With Structural and Functional Outcomes in Neovascular Age-Related Macular Degeneration

“…Nonetheless, the systemic immune alterations we observed are echoed by epidemiological links between AMD and systemic autoimmune diseases (e.g., higher AMD rates in Crohn's disease and lupus). 77 Moreover, our finding that peripheral T-cell profiles correlate with retinal fluid is in line with recent work by Lin et al, 78 which found altered systemic immune signaling associated with AMD severity (including nvAMD). This growing evidence supports a broader role for systemic immunity in AMD.…”

Elevation of Granulocyte Colony Stimulating Factor in Human AMD Donor RPE-Choroid

“…21 Cytokines signal to immune cells promoting inflammation and draw mononuclear cells to sites of tissue injury. A recent study has identified that local alterations in the extracellular matrix protein CYR61 in choroidal veins may be involved in the recruitment of circulating monocytes, 22 supporting the hypothesis that local alterations in inflammatory molecules may contribute to increased mononuclear cell infiltration and activation in AMD and thus could be responsible for the increased numbers of activated macrophages observed in the submacular region of AMD donor eyes. 4 Although there is significant evidence for a local inflammatory response in early/intermediate AMD, less is known about local alterations in inflammatory cytokines in the human RPE and choroid.…”

Type V Collagen Directs the Differentiation of iPSCs into Valvular Endothelial‐Like Cells

“…PCA and cluster analysis (Figure S9A,B) suggested that there were excellent intra-group similarities and significant differences between the two groups, which ensured the feasibility of subsequent histological analysis and the accuracy of the data. To further validate the role of Col V and assess the similarity between hVECs and iVEC-C, we performed hierarchical clustering analysis on five endothelial cell groups, including iVEC-M, iVEC-C, primary hVECs (HVEC: PRJNA552159), human aortic endothelial cells (HAEC: GSE89970), and human umbilical vein endothelial cells (HUVEC: GSE222101) [48][49][50]. The hierarchical clustering analysis and Spearman correlation analysis indicated that iVEC-C transcriptionally aligns more closely with primary hVECs than with other vascular endothelial subtypes (Figure S9C,D).…”