Targeting Acetylcholinesterase to Membrane Rafts

Xie, Heidi Qunhui; Liang, Dong; Leung, Ka Wing; Chen, Vicky P.; Zhu, Kevin Y.; Chan, Wallace K.B.; Choi, Roy Chi Yan; Massoulié, Jean; Tsim, Karl Wah Keung

doi:10.1074/jbc.m109.038711

Cited by 46 publications

(24 citation statements)

References 53 publications

(75 reference statements)

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“…This CRAC motif, defined by the algorithm (L/V)X 1-5 YX 1-5 (R/K), was further described in several other proteins that associate with cholesterol, including CYP11A1 (25), caveolin (29), acetylcholine esterase (31), and the gp41 peptide of the HIV-1 virus (30). Moreover, this motif is very often associated with the functionality of the protein in question.…”

Section: Discussionmentioning

confidence: 88%

Novel Androstenetriol Interacts with the Mitochondrial Translocator Protein and Controls Steroidogenesis

Midzak

Akula

Lecanu

et al. 2011

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 88%

Novel Androstenetriol Interacts with the Mitochondrial Translocator Protein and Controls Steroidogenesis

Midzak

Akula

Lecanu

et al. 2011

Journal of Biological Chemistry

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“…The CRAC motif is present in caveolins and in proteins targeted to lipid rafts or regulating cholesterol metabolism and transport [52]. The caveolin binding motif is found in proteins that interact with the caveolin scaffolding domain [45].…”

Section: Discussionmentioning

confidence: 99%

Popeye Domain Containing 1 (Popdc1/Bves) Is a Caveolae-Associated Protein Involved in Ischemia Tolerance

et al. 2013

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Popeye domain containing1 (Popdc1), also named Bves, is an evolutionary conserved membrane protein. Despite its high expression level in the heart little is known about its membrane localization and cardiac functions. The study examined the hypothesis that Popdc1 might be associated with the caveolae and play a role in myocardial ischemia tolerance. To address these issues, we analyzed hearts and cardiomyocytes of wild type and Popdc1-null mice. Immunoconfocal microscopy revealed co-localization of Popdc1 with caveolin3 in the sarcolemma, intercalated discs and T-tubules and with costameric vinculin. Popdc1 was co-immunoprecipitated with caveolin3 from cardiomyocytes and from transfected COS7 cells and was co-sedimented with caveolin3 in equilibrium density gradients. Caveolae disruption by methyl-β-cyclodextrin or by ischemia/reperfusion (I/R) abolished the cellular co-localization of Popdc1 with caveolin3 and modified their density co-sedimentation. The caveolin3-rich fractions of Popdc1-null hearts redistributed to fractions of lower buoyant density. Electron microscopy showed a statistically significant 70% reduction in caveolae number and a 12% increase in the average diameter of the remaining caveolae in the mutant hearts. In accordance with these changes, Popdc1-null cardiomyocytes displayed impaired [Ca+2]i transients, increased vulnerability to oxidative stress and no pharmacologic preconditioning. In addition, induction of I/R injury to Langendorff-perfused hearts indicated a significantly lower functional recovery in the mutant compared with wild type hearts while their infarct size was larger. No improvement in functional recovery was observed in Popdc1-null hearts following ischemic preconditioning. The results indicate that Popdc1 is a caveolae-associated protein important for the preservation of caveolae structural and functional integrity and for heart protection.

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“…31,35 These genes are proven to play an important role in colorectal cancer development, and altered DNA methylation patterns of these genes have already been reported in CRC tissue samples. The protein product of PRIMA1 anchors acetylcholinesterase to cell membranes, 36 while SDC2 encodes a transmembrane proteoglycan molecule participating in cell migration, proliferation, and cell-matrix interaction and acts as a cell-to-cell signaling molecule, such as a growth factor. 37 Hypermethylation of sequences in the promoter regions of these genes has already been reported in CRC tissue specimens; however, limited information is available from adenoma tissues.…”

Section: Introductionmentioning

confidence: 99%

Colorectal adenoma and cancer detection based on altered methylation pattern of SFRP1, SFRP2, SDC2, and PRIMA1 in plasma samples

et al. 2017

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Aberrant methylation is one of the most frequent epigenetic alterations that can contribute to tumor formation. Cell-free DNA can originate from tumor tissue; therefore, the evaluation of methylation markers in cell-free DNA can be a promising method for cancer screening. Our aim was to develop a panel of biomarkers with altered methylation along the colorectal adenoma-carcinoma sequence in both colonic tissue and plasma. Methylation of selected CpG sites in healthy colonic (n = 15), adenoma (n = 15), and colorectal cancer (n = 15) tissues was analyzed by pyrosequencing. MethyLight PCR was applied to study the DNA methylation of SFRP1, SFRP2, SDC2, and PRIMA1 gene promoters in 121 plasma and 32 biopsy samples. The effect of altered promoter methylation on protein expression was examined by immunohistochemistry. Significantly higher (P < 0.05) DNA methylation levels were detected in the promoter regions of all 4 markers, both in CRC and adenoma tissues compared with healthy controls. Methylation of SFRP1, SFRP2, SDC2, and PRIMA1 promoter sequences was observed in 85.1%, 72.3%, 89.4%, and 80.9% of plasma samples from patients with CRC and 89.2%, 83.8%, 81.1% and 70.3% from adenoma patients, respectively. When applied as a panel, CRC patients could be distinguished from controls with 91.5% sensitivity and 97.3% specificity [area under the curve (AUC) = 0.978], while adenoma samples could be differentiated with 89.2% sensitivity and 86.5% specificity (AUC = 0.937). Immunohistochemical analysis indicated decreasing protein levels of all 4 markers along the colorectal adenoma-carcinoma sequence. Our findings suggest that this methylation biomarker panel allows non-invasive detection of colorectal adenoma and cancer from plasma samples.

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Targeting Acetylcholinesterase to Membrane Rafts

Cited by 46 publications

References 53 publications

Novel Androstenetriol Interacts with the Mitochondrial Translocator Protein and Controls Steroidogenesis

Novel Androstenetriol Interacts with the Mitochondrial Translocator Protein and Controls Steroidogenesis

Popeye Domain Containing 1 (Popdc1/Bves) Is a Caveolae-Associated Protein Involved in Ischemia Tolerance

Colorectal adenoma and cancer detection based on altered methylation pattern of SFRP1, SFRP2, SDC2, and PRIMA1 in plasma samples

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