Target-triggered DNAzyme walker with 3D walking unit for copper species sensing in serum: A multivalent binding strategy for improving the detection performance

Zhou, Lihong; Xiong, Yamin; Wang, Hui; Yin, Aihong; Zhang, Xueying; Li, Huiling; Fu, Qinghua; Huang, Peili

doi:10.1016/j.snb.2021.129589

Cited by 13 publications

(7 citation statements)

References 41 publications

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“…Then, the DNAzyme leaves the fragments to bind with another substrate and repeats the hydrolysis procedure. As one DNAzyme will hydrolyze multiple substrates, there will be a signal amplification. ,, …”

Section: Resultsmentioning

confidence: 99%

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Endonuclease IV-Regulated DNAzyme Motor for Universal Single-nucleotide Variation Discrimination

Zhang

Yuan

et al. 2021

Anal. Chem.

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Single-nucleotide variation (SNV) detection plays significant roles in disease diagnosis and treatment. Generally, auxiliary probe, restricted design rules, complicated detection system, and repeated experimental parameter optimization are needed to obtain satisfactory tradeoff between sensitivity and selectivity for SNV discrimination, especially when different mutant sites need to be distinguished. To overcome these limitations, we developed a universal, straightforward, and relatively cheap SNV discrimination strategy, which simultaneously possessed high sensitivity and selectivity. The excellent performance of this strategy was ascribed to the SNV discrimination property of endonuclease IV (Endo IV) and the different hydrolysis behavior between free deoxyribozyme (DNAzyme) and the trapped DNAzyme to the substrates modified on gold nanoparticles (AuNPs). When Endo IV recognized the mutant-type target (MT), free DNAzyme was released from the probe, and the DNAzyme motor was activated with the help of cofactor Mn2+ to generate an amplified fluorescence signal. On the contrary, the wild-type target (WT) could not effectively trigger the DNAzyme motor. Moreover, for different SNV types, the corresponding probe could be designed by simply changing the sequence hybridized with the target and retaining the DNAzyme sequence. Thus, the fluorescence signal generation system does not need to change for different SNV targets. Five clinical-related SNVs were determined with the limit of detection (LOD) ranging from 0.01 to 0.05%, which exhibited competitive sensitivity over existing SNV detection methods. This strategy provided another insight into the properties of Endo IV and DNAzyme, expanded the applications of DNAzyme motor, and has great potential to be used for precision medicine.

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Section: Resultsmentioning

confidence: 99%

“…As one DNAzyme will hydrolyze multiple substrates, there will be a signal amplification. 28,40,41 We first inspected whether the substrate (D-Sub-1 in our experiment) was successfully modified on AuNPs. The D-Sub-1-modified AuNPs were named Sub-AuNPs.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Endonuclease IV-Regulated DNAzyme Motor for Universal Single-nucleotide Variation Discrimination

Zhang

Yuan

et al. 2021

Anal. Chem.

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“…Bacterial contamination and excessive metal ion-containing edibles are the main culprits, which means that developing rapid and reliable detection strategies is essential to prevent the occurrence of such diseases. 42,43 Xiong et al proposed an electrochemical biosensor using glass carbon electrodes modified with nitrogen-doped graphene, gold nanoparticles, and DNAzymes to detect the concentration of Ca 2+ in the serum of dairy cows. 44 The developed biosensor exhibited an ultrasensitive sensing ability with a detection limit of 3.8 × 10 −6 M, which presented a high potential to diagnose subclinical hypocalcemia.…”

Section: Extracellular Biosensingmentioning

confidence: 99%

RNA-cleaving DNAzymes for accurate biosensing and gene therapy

Gao¹,

Liu²,

Huo³

et al. 2023

Nanoscale

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“…Different from the above walking machines, multipedal 3D walkers possess more than two walking strands, including a tripod, tetrapod, or even more, that can migrate along the predesigned tracks at an extremely high moving rate and show superior amplification efficiency. 49 Multi-sequence structures, 50 multi-site molecular elements, 51,52 and various 3D metal materials, 53,54 can be used to develop multipedal walkers. It should be noted that if the amount of walking strands covers the surface of 3D materials, the DNA walker is also vividly called the DNA rolling machine.…”

Section: Influence Of Multiple Factors On 3d Walkersmentioning

confidence: 99%

The rate-limiting procedure of 3D DNA walkers and their applications in tandem technology

Chen

et al. 2023

Chem. Commun.

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Target-triggered DNAzyme walker with 3D walking unit for copper species sensing in serum: A multivalent binding strategy for improving the detection performance

Cited by 13 publications

References 41 publications

Endonuclease IV-Regulated DNAzyme Motor for Universal Single-nucleotide Variation Discrimination

Endonuclease IV-Regulated DNAzyme Motor for Universal Single-nucleotide Variation Discrimination

RNA-cleaving DNAzymes for accurate biosensing and gene therapy

The rate-limiting procedure of 3D DNA walkers and their applications in tandem technology

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