Target-swiped DNA lock for electrochemical sensing of miRNAs based on DNAzyme-assisted primer-generation amplification

Wang, Yeru; Sun, Wenyu; Zhang, Manru; Jiang, Long; Zhu, Zhixue; Li, Jingjing; Xu, Wanqing; Zhang, Qingxin; Li, Ming-Han; Chen, Zhihao; Liu, Su; Zhang, Fenfen; Wang, Yu; Liu, Su; Yu, Jinghua

doi:10.1007/s00604-021-04815-z

Cited by 5 publications

(3 citation statements)

References 30 publications

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“…The long-chain DNA produced by RCA can be combined with more signal probes to multiply the detection signal. Wang et al developed a nucleic acid lock (NAL) nanostructure and combined NAL with RCA technology to construct an electrochemical biosensor for highly sensitive detection of miRNA-21 [59]. First, the DNAzyme chain is used as the NAL structure, which is very stable, and miRNA-21 can specifically open the NAL structure.…”

Section: Nucleic Acid-based Signal Amplificationmentioning

confidence: 99%

Signal Amplification-Based Biosensors and Application in RNA Tumor Markers

Zhang

Gan

et al. 2023

Sensors

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Tumor markers are important substances for assessing cancer development. In recent years, RNA tumor markers have attracted significant attention, and studies have shown that their abnormal expression of post-transcriptional regulatory genes is associated with tumor progression. Therefore, RNA tumor markers are considered as potential targets in clinical diagnosis and prognosis. Many studies show that biosensors have good application prospects in the field of medical diagnosis. The application of biosensors in RNA tumor markers is developing rapidly. These sensors have the advantages of high sensitivity, excellent selectivity, and convenience. However, the detection abundance of RNA tumor markers is low. In order to improve the detection sensitivity, researchers have developed a variety of signal amplification strategies to enhance the detection signal. In this review, after a brief introduction of the sensing principles and designs of different biosensing platforms, we will summarize the latest research progress of electrochemical, photoelectrochemical, and fluorescent biosensors based on signal amplification strategies for detecting RNA tumor markers. This review provides a high sensitivity and good selectivity sensing platform for early-stage cancer research. It provides a new idea for the development of accurate, sensitive, and convenient biological analysis in the future, which can be used for the early diagnosis and monitoring of cancer and contribute to the reduction in the mortality rate.

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Section: Nucleic Acid-based Signal Amplificationmentioning

confidence: 99%

Signal Amplification-Based Biosensors and Application in RNA Tumor Markers

Zhang

Gan

et al. 2023

Sensors

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“…31,32 In addition, DNAzymes possess several unique properties, such as high catalytic activity, multiple turnover capability and being free of stringent reaction conditions. [33][34][35][36] These advantages make DNAzymes attractive signal amplification alternatives for the construction of various sensitive biosensors. Here, using a new self-constrained DNAzyme and HCR, we report a dually amplified and label-free fluorescence aptamer sensor to sensitively detect sarafloxacin in diluted milk samples.…”

Section: Introductionmentioning

confidence: 99%

Self-constrained DNAzyme for aptamer-based and sensitive label-free fluorescent assay of sarafloxacin via signal amplification cascades

Wang

Zhang

Yuan

et al. 2023

Analyst

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“…To address this problem, DNA cascade circuits have thus been constructed by integrating HCR circuits with other amplification methods and exhibit an improved amplification efficiency. − However, these cascade circuits are restricted by the limited amplification depth, resulting from their conventional stacked-configuration format. To overcome the abovementioned limitation, a more advanced initiator-regeneration mechanism has been applied to promote the amplification efficiency by automatically accumulating new triggers that facilitate the fast accomplishment of the self-sustainable DNA circuit systems, − yet current existing approaches always require complicated signal translation processes, resulting in the false signal leakage. Accordingly, it is highly desirable to devise a simple and robust strategy for the construction of autonomous initiator-duplicated amplifiers, which can significantly improve the amplification efficiency of the self-sustainable pathway.…”

Section: Introductionmentioning

confidence: 99%

Enzyme-Free Autocatalysis-Driven Feedback DNA Circuits for Amplified Aptasensing of Living Cells

Gao

Chen

Shang

et al. 2022

ACS Appl. Mater. Interfaces

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Aptasensors with high specificity have emerged as powerful tools for understanding various biological processes, thus providing tremendous opportunities for clinical diagnosis and prognosis. However, their applications in intracellular molecular imaging are largely impeded due to the low anti-interference capacity in biological environments and the moderate sensitivity to targets. Herein, a robust enzyme-free autocatalysis-driven feedback DNA circuit is devised for amplified aptasensing, for example, adenosine triphosphate (ATP) and thrombin, with a significantly improved sensitivity in living cells. This initiator-replicated hybridization chain reaction (ID-HCR) circuit was acquired by integrating the HCR circuit with the DNAzyme biocatalysis. Also, the autocatalysis-driven aptasensor consists of a recognition element and an amplification element. The recognition unit can specifically identify ATP or thrombin via a versatile conformational transformation, resulting in the exposure of the initiator to the autocatalysis-driven circuit. The ID-HCR element integrates the charming self-assembly characteristics of the HCR and the remarkable catalytic cleavage capacity of DNAzyme for realizing the continuously self-sustained regeneration or replication of trigger strands and for achieving an exponential signal gain. The autocatalysis-driven aptasensor has been validated for quantitative analysis of ATP and thrombin in vitro and for monitoring the corresponding aptamer substrates with various expressions in live cells. More importantly, the autocatalysis-driven aptasensor, as a versatile amplification strategy, holds enormous potential for analysis of other less abundant biomarkers by changing only the recognition element of the system.

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Target-swiped DNA lock for electrochemical sensing of miRNAs based on DNAzyme-assisted primer-generation amplification

Cited by 5 publications

References 30 publications

Signal Amplification-Based Biosensors and Application in RNA Tumor Markers

Signal Amplification-Based Biosensors and Application in RNA Tumor Markers

Self-constrained DNAzyme for aptamer-based and sensitive label-free fluorescent assay of sarafloxacin via signal amplification cascades

Enzyme-Free Autocatalysis-Driven Feedback DNA Circuits for Amplified Aptasensing of Living Cells

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