Target-responsive aptamer-cross-linked hydrogel sensors for the visual quantitative detection of aflatoxin B1 using exonuclease I-Triggered target cyclic amplification

Zheng, Mengyao; Li, Hongmei; Ye, Jin; Ni, Baoxia; Xie, Yanli; Wang, Songxue

doi:10.1016/j.fochx.2022.100395

Cited by 9 publications

(4 citation statements)

References 35 publications

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“…According to Chinese Food Hygiene Standard regulations, 20 µg/kg of AFB 1 is permitted in corn, peanuts, and peanut oil. For AFB 1 detection of DNA hydrogel biosensors, there are those using pt nanoparticles (ptNPs) catalyzed H 2 O 2 decomposition methods [ 158 ], those using urease catalyzed urea methods [ 159 ] and those using HRP catalyzed TMB discoloration [ 126 ] ( Figure 5 D). All these methods are based on the specific binding of aptamers leading to hydrogel collapse, but the difference lies in the way the signal is converted and analyzed.…”

Section: Stimulus-responsive Dna Hydrogel Biosensors For Food Safety ...mentioning

confidence: 99%

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Stimulus-Responsive DNA Hydrogel Biosensors for Food Safety Detection

Wang

Lai

et al. 2023

Biosensors

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Food safety has always been a major global challenge to human health and the effective detection of harmful substances in food can reduce the risk to human health. However, the food industry has been plagued by a lack of effective and sensitive safety monitoring methods due to the tension between the cost and effectiveness of monitoring. DNA-based hydrogels combine the advantages of biocompatibility, programmability, the molecular recognition of DNA molecules, and the hydrophilicity of hydrogels, making them a hotspot in the research field of new nanomaterials. The stimulus response property greatly broadens the function and application range of DNA hydrogel. In recent years, DNA hydrogels based on stimulus-responsive mechanisms have been widely applied in the field of biosensing for the detection of a variety of target substances, including various food contaminants. In this review, we describe the recent advances in the preparation of stimuli-responsive DNA hydrogels, highlighting the progress of its application in food safety detection. Finally, we also discuss the challenges and future application of stimulus-responsive DNA hydrogels.

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Section: Stimulus-responsive Dna Hydrogel Biosensors For Food Safety ...mentioning

confidence: 99%

“…( D ) Detection principle of AFB 1 -responsive aptamer-functionalized DNA intelligent hydrogel biosensor. Copyright (2020) Elsevier [ 126 ].…”

Section: Figurementioning

confidence: 99%

Stimulus-Responsive DNA Hydrogel Biosensors for Food Safety Detection

Wang

Lai

et al. 2023

Biosensors

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“…Thus, a novel sample preparation method is highly desirable. In particular, aptamers [28][29][30], molecularly imprinted polymers [31][32][33], and a novel receptor with antibody-like properties have been employed in applications ranging from separation systems to sensors.…”

Section: Introductionmentioning

confidence: 99%

Development of a Novel Magnetic-Bead-Based Automated Strategy for Efficient and Low-Cost Sample Preparation for Ochratoxin A Detection Using Mycotoxin–Albumin Interaction

Jin

Bao

Zheng

et al. 2023

Toxins

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The mycotoxin ochratoxin A (OTA) is toxic to humans and frequently contaminates wine and beer. Antibodies are essential recognition probes for the detection of OTA. However, they have several drawbacks, such as high costs and difficulty in preparation. In this study, a novel magnetic-bead-based automated strategy for efficient and low-cost OTA sample preparation was developed. Human serum albumin, which is an economical and stable receptor based on the mycotoxin–albumin interaction, was adapted and validated to replace conventional antibodies to capture OTA in the sample. Ultra-performance liquid chromatography–fluorescence detection was used in combination with this preparation method for efficient detection. The effects of different conditions on this method were investigated. The recovery of OTA samples spiked at three different concentrations ranged from 91.2% to 102.1%, and the relative standard deviations (RSDs) were 1.2%–8.2% in wine and beer. For red wine and beer samples, the LODs were 0.37 and 0.15 µg/L, respectively. This reliable method overcomes the drawbacks of conventional methods and offers significant application prospects.

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“…11 An "aptamer" is a segment of single-stranded (ss)DNA or RNA that can fold into a particular form to recognize targets such as enzymes, 12 antibodies, 13 metal ions, 14 and biotoxins. 15 These aptamers have satisfactory specicity and affinity for their targets, as well as accessibility for synthesis and modication. 16 Various "aptasensors" have been investigated by fusing different types of signal, including uorescence assays, electrochemical methods, surface-enhanced Raman spectroscopy, and photoelectrochemical methods.…”

Section: Introductionmentioning

confidence: 99%

A novel colorimetric assay for sensitive detection of kanamycin based on the aptamer-regulated peroxidase-mimicking activity of Co₃O₄ nanoparticles

Zhou

et al. 2023

Anal. Methods

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Target-responsive aptamer-cross-linked hydrogel sensors for the visual quantitative detection of aflatoxin B1 using exonuclease I-Triggered target cyclic amplification

Cited by 9 publications

References 35 publications

Stimulus-Responsive DNA Hydrogel Biosensors for Food Safety Detection

Stimulus-Responsive DNA Hydrogel Biosensors for Food Safety Detection

Development of a Novel Magnetic-Bead-Based Automated Strategy for Efficient and Low-Cost Sample Preparation for Ochratoxin A Detection Using Mycotoxin–Albumin Interaction

A novel colorimetric assay for sensitive detection of kanamycin based on the aptamer-regulated peroxidase-mimicking activity of Co₃O₄ nanoparticles

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Target-responsive aptamer-cross-linked hydrogel sensors for the visual quantitative detection of aflatoxin B1 using exonuclease I-Triggered target cyclic amplification

Cited by 9 publications

References 35 publications

Stimulus-Responsive DNA Hydrogel Biosensors for Food Safety Detection

Stimulus-Responsive DNA Hydrogel Biosensors for Food Safety Detection

Development of a Novel Magnetic-Bead-Based Automated Strategy for Efficient and Low-Cost Sample Preparation for Ochratoxin A Detection Using Mycotoxin–Albumin Interaction

A novel colorimetric assay for sensitive detection of kanamycin based on the aptamer-regulated peroxidase-mimicking activity of Co3O4 nanoparticles

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A novel colorimetric assay for sensitive detection of kanamycin based on the aptamer-regulated peroxidase-mimicking activity of Co₃O₄ nanoparticles