Tannic acid modulates intestinal barrier functions associated with intestinal morphology, antioxidative activity, and intestinal tight junction in a diquat-induced mouse model

Wang, Meiwei; Huang, Huijun; Liu, Shuang; Yu, Zhuang; Yang, Huansheng; Li, Yali; Chen, Shuai; Wang, Lixia; Yin, Lanmei; Yao, Yuanfeng; He, Shanping

doi:10.1039/c9ra04943f

Cited by 25 publications

(23 citation statements)

References 47 publications

(51 reference statements)

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“…Many studies were carried out with TA in mouse models where a concentration of 30 mg/kg of TA was used in PSAPP mice [44]. Another study showed that treatment with 10 mg kg −1 TA along with diquat in mice induced a non-significant difference in the mice body weight [45]. Targeting these cancer stem cells is a better method of cancer chemotherapy, as it prevents cancer recurrence by attenuating the formation of the cancer stem cells.…”

Section: Discussionmentioning

confidence: 99%

Tannic Acid Promotes TRAIL-Induced Extrinsic Apoptosis by Regulating Mitochondrial ROS in Human Embryonic Carcinoma Cells

Kang

et al. 2020

Cells

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Human embryonic carcinoma (EC; NCCIT) cells have self-renewal ability and pluripotency. Cancer stem cell markers are highly expressed in NCCIT cells, imparting them with the pluripotent nature to differentiate into other cancer types, including breast cancer. As one of the main cancer stem cell pathways, Wnt/β-catenin is also overexpressed in NCCIT cells. Thus, inhibition of these pathways defines the ability of a drug to target cancer stem cells. Tannic acid (TA) is a natural polyphenol present in foods, fruits, and vegetables that has anti-cancer activity. Through Western blotting and PCR, we demonstrate that TA inhibits cancer stem cell markers and the Wnt/β-catenin signaling pathway in NCCIT cells and through a fluorescence-activated cell sorting analysis we demonstrated that TA induces sub-G1 cell cycle arrest and apoptosis. The mechanism underlying this is the induction of mitochondrial reactive oxygen species (ROS) (mROS), which then induce the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated extrinsic apoptosis pathway instead of intrinsic mitochondrial apoptosis pathway. Moreover, ribonucleic acid sequencing data with TA in NCCIT cells show an elevation in TRAIL-induced extrinsic apoptosis, which we confirm by Western blotting and real-time PCR. The induction of human TRAIL also proves that TA can induce extrinsic apoptosis in NCCIT cells by regulating mROS.

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Section: Discussionmentioning

confidence: 99%

Tannic Acid Promotes TRAIL-Induced Extrinsic Apoptosis by Regulating Mitochondrial ROS in Human Embryonic Carcinoma Cells

Kang

et al. 2020

Cells

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“…Western blotting was performed as described in our previous study ( Wang et al, 2019 ). Tween-20, RIPA buffer, protease inhibitor cocktail, bicinchoninic acid assay, 5 × loading buffer, SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) gels kit, and horseradish peroxidase-linked secondary antibodies were purchased from Beyotime Biotechnology (Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

Herbal Extract Mixture Modulates Intestinal Antioxidative Capacity and Microbiota in Weaning Piglets

et al. 2021

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Recently, herbal extracts have been applied in multiple aspects, such as medicine and animal feed. Different compositions of herbal extract mixture (HEM) have various components and diverse functions. This study aimed to evaluate the effects of HEM (Lonicera japonica, Astragalus membranaceus, Eucommia folium, and Codonopsis pilosula) on intestinal antioxidant capacity and colonic microbiota in weaned pigs. A total of 18 piglets [Duroc × (Landrace × Yorkshire)] with the initial body weight of 5.99 ± 0.13 kg (weaned at 21 days) were randomly divided into two groups (n = 9): the control group (CON, basal diet) and the HEM treatment group (HEM, 1,000 mg/kg HEM + basal diet). The experiment period lasted for 14 days. Our results showed that dietary supplementation with HEM modulated the antioxidant capacity through decreasing the activity of superoxide dismutase (SOD) in the ileum and glutathione peroxidase (GSH-PX) in the serum, and decreasing the mRNA expression of Kelch like-ECH-associated protein 1 (Keap1) in the jejunum and the protein level of Keap1 in the ileum. Moreover, the HEM group modified the composition of colonic microbiota with affecting relative abundances of the Firmicutes and Bacteroidetes at the phylum level. Taken together, supplementation of HEM can regulate the antioxidant capacity and modify the composition of colonic bacteria in weaning piglets. This study provides new insights into the combination effects of herbal extracts on weaning piglets.

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“…Intestinal inflammation caused barrier breakdown leading to the inflammation of the intestinal mucosa and impairment of integrity, allowing pathogenic bacteria, toxins, and antigens from the lumen to translocate into the bloodstream and cause several dysfunctional conditions [ 103 ]. Several studies have reported that polyphenols have the potential to modulate intestinal permeability [ 104 ], and although not directly tested with polyphenols from mango, the intake of gallotannins was shown to improve intestinal barrier integrity and function through the increase in zonula occludens-1 (ZO-1) mRNA expression in oxidative stress induced in a mouse model [ 105 ]. In another study of weaning piglets with a high frequency of diarrhea due to impaired tight junctions, tannic acid intake reduced diarrhea by up-regulating the expression of occludin and zonula occudens-2 (ZO-2) mRNA [ 106 ].…”

Section: Mango Polyphenols In Gastrointestinal Disordersmentioning

confidence: 99%

Mango (Mangifera indica L.) Polyphenols: Anti-Inflammatory Intestinal Microbial Health Benefits, and Associated Mechanisms of Actions

et al. 2021

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Mango is rich in polyphenols including gallotannins and gallic acid, among others. The bioavailability of mango polyphenols, especially polymeric gallotannins, is largely dependent on the intestinal microbiota, where the generation of absorbable metabolites depends on microbial enzymes. Mango polyphenols can favorably modulate bacteria associated with the production of bioactive gallotannin metabolites including Lactobacillus plantarum, resulting in intestinal health benefits. In several studies, the prebiotic effects of mango polyphenols and dietary fiber, their potential contribution to lower intestinal inflammation and promotion of intestinal integrity have been demonstrated. Additionally, polyphenols occurring in mango have some potential to interact with intestinal and less likely with hepatic enzymes or transporter systems. This review provides an overview of interactions of mango polyphenols with the intestinal microbiome, associated health benefits and underlying mechanisms.

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Tannic acid modulates intestinal barrier functions associated with intestinal morphology, antioxidative activity, and intestinal tight junction in a diquat-induced mouse model

Abstract: The concentration of 2.5 mg kg−1 TA can ameliorate diquat-challenged jejunal injury in mice.

Cited by 25 publications

References 47 publications

Tannic Acid Promotes TRAIL-Induced Extrinsic Apoptosis by Regulating Mitochondrial ROS in Human Embryonic Carcinoma Cells

Tannic Acid Promotes TRAIL-Induced Extrinsic Apoptosis by Regulating Mitochondrial ROS in Human Embryonic Carcinoma Cells

Herbal Extract Mixture Modulates Intestinal Antioxidative Capacity and Microbiota in Weaning Piglets

Mango (Mangifera indica L.) Polyphenols: Anti-Inflammatory Intestinal Microbial Health Benefits, and Associated Mechanisms of Actions

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