Tandem separation schemes for preparative high-performance liquid chromatography of proteins

Characterizing, and Compensating for, Enrichment Effects in Displacement Chromatography with Multicomponent Carriers

“…Table 2 lists the results of a representative numerical calculation, where the displacer front gives rise to a modulatorrich zone, with an enrichment of 16% relative to the mobile phase modulator concentration at the inlet. This is an appreciable increase, in view of the sensitive dependence of biopolymer retention on salt concentration in the mobile phase (Jennissen, 1976; Lee et al, 1988). Similar results have been obtained experimentally by Gadam et al (1993).…”

Characterizing, and Compensating for, Enrichment Effects in Displacement Chromatography with Multicomponent Carriers

“…Table 2 lists the results of a representative numerical calculation, where the displacer front gives rise to a modulatorrich zone, with an enrichment of 16% relative to the mobile phase modulator concentration at the inlet. This is an appreciable increase, in view of the sensitive dependence of biopolymer retention on salt concentration in the mobile phase (Jennissen, 1976; Lee et al, 1988). Similar results have been obtained experimentally by Gadam et al (1993).…”

Potential O‐Acyl‐Substituted (−)‐Epicatechin Gallate Prodrugs as Inhibitors of DMBA/TPA‐Induced Squamous Cell Carcinoma of Skin in Swiss Albino Mice

“…For the synthesis of the O-acyl derivatives, the partially purified catechin fraction was treated with an acylating agent in the presence of pyridine (Scheme). The acylated material was subjected to tandem preparative-scale elution chromatography (SGC I and SGC II) [27] and preparative TLC (PTLC), to obtain the O-acyl derivatives of (À)-ECG present in green tea. The structures of these compounds were determined by physical data and spectral studies.…”

Chemically Selective Displacers for High-Resolution Protein Separations in Ion-Exchange Systems: Effect of Displacer−Protein Interactions

“…Displacement chromatography has been successfully employed for the purification of proteins using hydroxyapatite, , hydrophobic interaction, and ion exchange chromatographic systems. − Ion exchange displacement chromatography in particular has attracted significant attention as a powerful technique for the purification of biomolecules. ,, A wide variety of classes of displacers, such as polyelectrolytes, polysaccharides, low-molecular-mass dendrimers, amino acids, antibiotics, and aminoglycoside-polyamine, has been identified for protein separations in ion-exchange systems. The application of low-molecular-mass displacers has attracted attention due to several distinct operational advantages, most significantly the ability of some of these molecules to act as mass action displacers. , …”

Characterizing, and Compensating for, Enrichment Effects in Displacement Chromatography with Multicomponent Carriers

“…Table 2 lists the results of a representative numerical calculation, where the displacer front gives rise to a modulatorrich zone, with an enrichment of 16% relative to the mobile phase modulator concentration at the inlet. This is an appreciable increase, in view of the sensitive dependence of biopolymer retention on salt concentration in the mobile phase (Jennissen, 1976; Lee et al, 1988). Similar results have been obtained experimentally by Gadam et al (1993).…”

Potential O‐Acyl‐Substituted (−)‐Epicatechin Gallate Prodrugs as Inhibitors of DMBA/TPA‐Induced Squamous Cell Carcinoma of Skin in Swiss Albino Mice

“…For the synthesis of the O-acyl derivatives, the partially purified catechin fraction was treated with an acylating agent in the presence of pyridine (Scheme). The acylated material was subjected to tandem preparative-scale elution chromatography (SGC I and SGC II) [27] and preparative TLC (PTLC), to obtain the O-acyl derivatives of (À)-ECG present in green tea. The structures of these compounds were determined by physical data and spectral studies.…”

Chemically Selective Displacers for High-Resolution Protein Separations in Ion-Exchange Systems: Effect of Displacer−Protein Interactions

“…Displacement chromatography has been successfully employed for the purification of proteins using hydroxyapatite, , hydrophobic interaction, and ion exchange chromatographic systems. − Ion exchange displacement chromatography in particular has attracted significant attention as a powerful technique for the purification of biomolecules. ,, A wide variety of classes of displacers, such as polyelectrolytes, polysaccharides, low-molecular-mass dendrimers, amino acids, antibiotics, and aminoglycoside-polyamine, has been identified for protein separations in ion-exchange systems. The application of low-molecular-mass displacers has attracted attention due to several distinct operational advantages, most significantly the ability of some of these molecules to act as mass action displacers. , …”

Characterizing, and Compensating for, Enrichment Effects in Displacement Chromatography with Multicomponent Carriers

“…Table 2 lists the results of a representative numerical calculation, where the displacer front gives rise to a modulatorrich zone, with an enrichment of 16% relative to the mobile phase modulator concentration at the inlet. This is an appreciable increase, in view of the sensitive dependence of biopolymer retention on salt concentration in the mobile phase (Jennissen, 1976; Lee et al, 1988). Similar results have been obtained experimentally by Gadam et al (1993).…”

Potential O‐Acyl‐Substituted (−)‐Epicatechin Gallate Prodrugs as Inhibitors of DMBA/TPA‐Induced Squamous Cell Carcinoma of Skin in Swiss Albino Mice

“…For the synthesis of the O-acyl derivatives, the partially purified catechin fraction was treated with an acylating agent in the presence of pyridine (Scheme). The acylated material was subjected to tandem preparative-scale elution chromatography (SGC I and SGC II) [27] and preparative TLC (PTLC), to obtain the O-acyl derivatives of (À)-ECG present in green tea. The structures of these compounds were determined by physical data and spectral studies.…”

Chemically Selective Displacers for High-Resolution Protein Separations in Ion-Exchange Systems: Effect of Displacer−Protein Interactions

“…Displacement chromatography has been successfully employed for the purification of proteins using hydroxyapatite, , hydrophobic interaction, and ion exchange chromatographic systems. − Ion exchange displacement chromatography in particular has attracted significant attention as a powerful technique for the purification of biomolecules. ,, A wide variety of classes of displacers, such as polyelectrolytes, polysaccharides, low-molecular-mass dendrimers, amino acids, antibiotics, and aminoglycoside-polyamine, has been identified for protein separations in ion-exchange systems. The application of low-molecular-mass displacers has attracted attention due to several distinct operational advantages, most significantly the ability of some of these molecules to act as mass action displacers. , …”