Tamoxifen citrate induced sperm shape abnormalities in the in vivo mouse

Padmalatha, S; Vijayalaxmi, K.K.

doi:10.1016/s1383-5718(00)00160-1

Cited by 25 publications

(10 citation statements)

References 30 publications

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“…Although organ weight and histopathology indicated substantial endocrine-mediated effects on the accessory male sex organs at 200 and 30 lg/kg per day, spermatological investigations did not reveal any remarkable changes. In a previous study, tamoxifen was shown to induce sperm shape abnormalities in Swiss albino mice 5 weeks after oral administration of 100 to 800 lg/kg per day over 10 days (Padmalatha Rai and Vijayalaxmi 2001). A possible reason why sperm parameters were not altered in the present study could be that the 4-week treatment is almost half the length of the 53-day spermatogonial cycle of the male rat, while in the study performed in mouse, effects were observed after a complete 35-day spermatogonial cycle (Padmalatha Rai and Vijayalaxmi 2001).…”

Section: Discussionmentioning

confidence: 98%

“…In a previous study, tamoxifen was shown to induce sperm shape abnormalities in Swiss albino mice 5 weeks after oral administration of 100 to 800 lg/kg per day over 10 days (Padmalatha Rai and Vijayalaxmi 2001). A possible reason why sperm parameters were not altered in the present study could be that the 4-week treatment is almost half the length of the 53-day spermatogonial cycle of the male rat, while in the study performed in mouse, effects were observed after a complete 35-day spermatogonial cycle (Padmalatha Rai and Vijayalaxmi 2001). Elsewhere, it has been suggested that estrogens play a role in the male reproductive system and especially in the process of concentration, maturation, and survival of sperm in the epididymis (Hess et al 1997(Hess et al , 2001Sharpe 1997).…”

Section: Discussionmentioning

confidence: 98%

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Tamoxifen: 28-day oral toxicity study in the rat based on the Enhanced OECD Test Guideline 407 to detect endocrine effects

Kennel

Pallen

Barale-Thomas

et al. 2003

Archives of Toxicology

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The main objective of this 28-day oral gavage toxicity study in the rat was to investigate which of the current and/or additional parameters of the OECD Test Guideline 407 would reliably and sensitively detect the endocrine-mediated effects of the nonsteroidal antiestrogen tamoxifen. In addition, as this study was performed using two subgroups of five animals of each sex run concurrently, it enabled an assessment of the intralaboratory reproducibility while also assessing the potential value of using ten animals of each sex per group instead of using the standard five animals of each sex per group stipulated by the current guideline. Tamoxifen was administered daily by gavage to groups of 7-week-old Wistar rats for at least 28 days at dose levels of 5, 30, or 200 microg/kg body weight. Additional parameters specified in the enhanced OECD Test Guideline 407 were spermatozoa enumeration and morphology of the cauda epididymis, hormonal analysis of the thyroid-stimulating hormone (TSH), triiodothyronine (T3) and thyroxine (T4) levels, monitoring of the estrous cycle during week 4 of treatment to ensure females were in diestrus on the day of terminal sacrifice, organ weight of ovary, uterus, thyroid gland, prostate gland (ventral and dorsolateral parts), seminal vesicles with coagulation glands and pituitary gland, and microscopic investigation of the pituitary gland, vagina, mammary gland, seminal vesicles with coagulating glands, epididymis, and prostate gland (ventral and dorsolateral parts). Overall, 200 microg/kg per day was considered to be the Maximum Tolerated Dose (MTD) in both sexes, resulting in a marked reduction of body weight gain, together with slight effects on clinical signs, hematology, plasma chemistry, and microscopic changes in some endocrine tissues. Five micrograms per kilogram per day represented the No Observed Adverse Effect Level (NOAEL) in males and the No Observed Effect Level (NOEL) in females. At the intermediate dose level (30 microg/kg per day), the current OECD Test Guideline 407 was appropriate to detect the specific endocrine-related changes induced by tamoxifen in females, based on the histopathology findings observed in the ovary and the uterus. The additional parameters which were found to be changed in females (thyroid hormone levels, ovary and uterus weights, and histopathology of vagina) provided supplementary information further confirming tamoxifen-mediated endocrine effects. In males, when data from the current Test Guideline 407 were considered at the intermediate dose level, specific endocrine effects were only indicated on the basis of the histopathology findings observed in the prostate gland. The additional parameters examined which were found to be changed (prostate gland and seminal vesicle weights, and histopathology of seminal vesicle and mammary gland) were necessary to confirm the specific tamoxifen-mediated endocrine effects. Hence, amongst the additional parameters contained in the enhanced OECD Test Guideline 407, organ weights and histopathological examinatio...

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 98%

Tamoxifen: 28-day oral toxicity study in the rat based on the Enhanced OECD Test Guideline 407 to detect endocrine effects

Kennel

Pallen

Barale-Thomas

et al. 2003

Archives of Toxicology

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show abstract

“…Sperm-shape test provides a direct measure of the quality of sperm production from animals under chemical treatment (Padmalatha Rai and Vijayalaxmi, 2001). The origin of abnormally shaped sperms is not completely understood; small deletions, point mutations and abnormal chromosomes, have been proposed as possible genetic causes of such alterations (Mendoza-Lujambio et al, 2002;Pyle and Handel, 2003;Escalier et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Assessment of the genotoxic risk of Punica granatum L. (Punicaceae) whole fruit extracts

Sánchez-Lamar¹,

Fonseca²,

Fuentes³

et al. 2008

Journal of Ethnopharmacology

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“…Cyclophosphamide ( Mouse sperm abnormality test Mouse sperm abnormality test was performed using the modification methods (Padmalatha and Vijayalaxmi, 2001;Ribeiro et al, 1987). Twenty-five healthy and sexually matured male Kunming mice were divided randomly into 5 groups.…”

Section: Ames Testmentioning

confidence: 99%

“…Eosine Y for 45 min, then the slides were air dried and coded (Padmalatha and Vijayalaxmi, 2001;Olufunmiso et al, 2011). For each suspension 1000 sperm were examined.…”

Section: Ames Testmentioning

confidence: 99%

Toxicological Assessment of Chinese Cherry (Cerasus Pseudocerasus L.) Seed Oil

Chen

Zhu

et al. 2014

FSTR

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Keywords: Chinese cherry (Cerasus pseudocerasus L.) seed oil, toxicology, safety IntroductionChinese cherry (Cerasus pseudocerasus L.), originating in China with many local cultivars for thousands of years, is widely distributed in the temperate zone of Northern Hemisphere (Li et al., 2009;Zhang et al., 2010). The biological characteristics of Chinese cherry are similar to sweet cherry (Cerasus avium) as perennial species belonging to the Rosaceae family. The cherry tree has shown a high added-value as a medicinal plant for a long time.For instance, in traditional system of medicine, the cherry fruits are being used to combat several diseases and pathological conditions, such as heart failure, beriberi, dropsy, and mastitis (Jung et al., 2002;Lee et al., 2007). Furthermore, antibacterial, antioxidative and anticancer activities of the extracts of cherry stems, leaves and blossoms have been extensively investigated in recent years (Lee et al., 2007;Piccirillo et al., 2010).Chinese cherry seed oil (CSO) comprises 11 _ 13% of total seed weight. The oil content of Chinese cherry seed is a little bit higher than that of sweet cherry seed, while the composition of fatty acids is not significant difference. The main fatty acids found in the seeds including oleic acid, linoleic acid and conjugated linolenic acids (Comes et al., 1992;Kamel and Kakuda, 1992; Takagi and Itabashi, 1981). Sweet cherry oil has been commercially available and used to product various cosmetics, some pharmaceutical preparations and salad oil (Jamieson et al., 1930;Rabak, 1932).It is crucial to evaluate the adverse effects of chemicals or complex mixtures for understanding the genotoxicity risk in a population (Hwang and Kim, 2011). As is known, gene mutation and chromosomal aberrations are two major end points ofgenotoxicity (Hayashi et al., 1994). The bacterial reverse mutation test is used world-widely as an initial screen to determine the mutagenic potential of new substance (Mortelmans and Zeiger, 2000). However, mammalian erythrocyte micronucleus test is usually used to evaluate structural and numerical chromosomal aberrations (Hayashi et al., 1994). Therefore, to definitively judge the safety of the active substance and give better prediction for human acute lethal dose, it would be necessary to use the toxicity results from acute oral toxicity test, mouse sperm abnormality test and repeated dose 28-day oral toxicity test (Jothy et al., 2008).Although Chinese cherry has been widely consumed for thousands of years and CSO has been widely consumed for decades of years, little is known about the possible toxicity and safety of the Chinese cherry or the CSO. Therefore, in the current study, the toxicology and safety of CSO was evaluated using the acute oral toxicity test, bacterial reverse mutation test, mammalian erythrocyte micronucleus test, mouse sperm abnormality test and repeated dose 28-day oral toxicity test. Materials and Methods Cherry seed oil (CSO) CSO was gained by pressing the clean cherry seeds of Chinese cherry cultivar Taixi...

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Tamoxifen citrate induced sperm shape abnormalities in the in vivo mouse

Cited by 25 publications

References 30 publications

Tamoxifen: 28-day oral toxicity study in the rat based on the Enhanced OECD Test Guideline 407 to detect endocrine effects

Tamoxifen: 28-day oral toxicity study in the rat based on the Enhanced OECD Test Guideline 407 to detect endocrine effects

Assessment of the genotoxic risk of Punica granatum L. (Punicaceae) whole fruit extracts

Toxicological Assessment of Chinese Cherry (Cerasus Pseudocerasus L.) Seed Oil

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