Tamarind kernel powder co-induces xylanase and cellulase production during submerged fermentation of Termitomyces clypeatus

Chatterjee, Ratna; Majumder, K.; Sengupta, S. K.

doi:10.1007/s12257-009-3042-z

Cited by 8 publications

(5 citation statements)

References 27 publications

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“…The mycelial culture of mushroom Pleurotus ostreatus was grown at 28 ± 1 °C for 5 days in a medium containing 1% glucose, 1% malt extract, 10% potato extract, and 0.15 % KH 2 PO 4 after inoculation with small mycelial pieces of the fungus. The enzyme production medium contained (g l −1 ) NH 4 H 2 PO 4 – 24, MgSO 4 .7H 2 O– 0.5, CaCl 2 .2H 2 O– 0.37, H 3 PO 4 – 0.57, FeSO 4 .7H 2 O– 0.25, MnCl 2 – 0.032, NaMoO 4 – 0.032, KH 2 PO 4 – 1.5 [ 7 ] in combination with a carbon source at 20 g l −1 . Mycelial pellets were then transferred aseptically to a 250 ml of polypropylene flask containing two 1-cm diameter glass beads and crushed to form a slurry mixture by shaking for 2 h. For submerged fermentation (smf), 0.4 gl −1 inoculums were added to a 50 ml enzyme production medium in a 250-ml Erlenmeyer flask, while for solid-state fermentation (ssf), the same quantity of inoculum was added to polyurethane foam (PUF) cubes of 1 g, absorbed with 50 ml of enzyme production medium in a 250-ml Erlenmeyer flask [ 8 ].…”

Section: Methodsmentioning

confidence: 99%

An analysis of exo-polygalacturonase bioprocess in submerged and solid-state fermentation by Pleurotus ostreatus using pomelo peel powder as carbon source

Majumder¹,

Paul²,

Sundas³

2020

Journal of Genetic Engineering and Biotechnology

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Background As there has been an increasing trend in the effective utilization of plant and crop residues for microbial transformation into a desired product, an attempt was made to compare of exo-polygalacturonase production using logistic and Luedeking-Piret kinetic model by Pleurotus ostreatus in submerged (smf) or solid-state fermentation (ssf) using pomelo peel powder, an agro-forestry residue as carbon substrate. Results Cultures grown in submerged fermentation produced a peak of exo-polygalacturonase activity as 6160 Ul-1 on the 4th day of culture as compared with 2410 Ul-1 on the 5th day of fermentation by solid-state fermentation. The enzyme yield coefficient (YE/X) is of higher value in smf vs. ssf system (YE/X = 1.05 × 103 vs. 0.622 × 103) indicating the more efficient product yield in smf as compared with ssf. The plots derived fromλ versusζ clearly demonstrate that the secondary product destruction is higher in smf than in ssf. Conclusion P. ostreatus performs much better in submerged fermentation as compared with solid-state fermentation in respect to exo-polygalacturonase production although ssf technique produced a more thermo-stable exo-polygalacturonase in crude extract, which is highly desirable in various industrial applications.

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Section: Methodsmentioning

confidence: 99%

An analysis of exo-polygalacturonase bioprocess in submerged and solid-state fermentation by Pleurotus ostreatus using pomelo peel powder as carbon source

Majumder¹,

Paul²,

Sundas³

2020

Journal of Genetic Engineering and Biotechnology

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“…Substrate optimization may induce cellulase production. Supplementing substrate induced cellulase production when TKP (tamarind kennel powder) was added to the basal medium (30). Therefore, it may be possible to produce cellulase with the highest activity when the optimization process was done with several factors including both physiological and nutritional parameters.…”

Section: Quantification Of Cellulase By Fpase and Cmcase Assaymentioning

confidence: 99%

A comparative study of cellulase production in inorganic and organic supplements by a cellulolytic tree bark fungus

Vijeetha¹,

Nair²

2020

Plant Sci. Today

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The study was conducted to compare the cellulase production using organic and inorganic supplements by isolating a tree bark (Mangifera indica) fungus, for a cost-effective cellulase production. Three fungi (f1, f2 and f3) were isolated from the bark of the tree, of which f3 was found to be potential in cellulase production which was confirmed by primary screening (congo red activity staining). Through endoglucanase and total cellulase activity assays, it was observed that the isolated strain exhibited cellulase activity of 0.25 U/ml for endoglucanase when 1% CMC was supplemented to the medium. An activity of 8 IU/g towards FPase and for endoglucanase 10.35 U/g was obtained on non-purified inorganic supplements. Sugarcane bagasse was found to be the best inorganic supplement for cellulase production. Here, we try to adopt a cost-effective production strategy of cellulase. The future perspective of this investigation is to identify the strain and purification of the enzyme for industrial purpose.

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“…However, the maximum mycelial growth and exopolysaccharide production in the culture of Paecilomyces japonica using maltose [37], Ganoderma lucidum using xylose, sorbitol and manitol, Daedalea aquercina using dextrin [38], and Pacilomyces japonica using maltose and sucrose [39] were obtained. In the case of Termitomyces clypeatus for production of both cellulolytic and xylanolytic enzymes in a submerged culture, tamarind kernel powder was also used [40].…”

Section: Effect Of Carbon Source On Mycelial Growth and Exopolysacchamentioning

confidence: 99%

A study of mycelial growth and exopolysaccharide production from a submerged culture of Mycoleptodonoides aitchisonii in an air-lift bioreactor

Choi

Lee

Kim

et al. 2011

Korean J. Chem. Eng.

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We determined the optimal culture and medium conditions for effective production of mycelial mass and exopolysaccharide from a liquid culture of Mycoleptodonoides aitchisonii in an air-lift bioreactor. The mycelial growth and exopolysaccharide production were found to be optimal at a temperature of 25 o C and pH of 6.5. When 60 g/L of lactose was used as a carbon source, the maximum mycelial growth and exopolysaccharide production were obtained. The polypeptone and yeast extract were the most appropriate nitrogen sources for mycelial growth and exopolysaccharide production. In addition, when a mixture of 20 g/L of polypeptone and 5 g/L of yeast extract was used, the exopolysaccharide production increased 50% compared to that of the sole nitrogen source. CaCl 2 ·2H 2 O (1.0 g/L) was the most effective mineral source. Using the optimal culture and medium conditions, batch cultures with basal and designed medium on mycelial growth and exopolysaccharide production in a 5 L air-lift bioreactor were carried out for 16 days. The mycelial growth and exopolysaccharide production increased with an increase of culture time at 14 days, and the maximum mycelial growth and exopolysaccharide production were 20.3 and 6.2 g/L, respectively, after 14 days of culture. The developed model in an air-lift bioreactor showed good agreement with experimental data. These results indicate that exopolysaccharide production is associated with the mycelial growth of M. aitchisonii in an air-lift bioreactor.

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Tamarind kernel powder co-induces xylanase and cellulase production during submerged fermentation of Termitomyces clypeatus

Cited by 8 publications

References 27 publications

An analysis of exo-polygalacturonase bioprocess in submerged and solid-state fermentation by Pleurotus ostreatus using pomelo peel powder as carbon source

An analysis of exo-polygalacturonase bioprocess in submerged and solid-state fermentation by Pleurotus ostreatus using pomelo peel powder as carbon source

A comparative study of cellulase production in inorganic and organic supplements by a cellulolytic tree bark fungus

A study of mycelial growth and exopolysaccharide production from a submerged culture of Mycoleptodonoides aitchisonii in an air-lift bioreactor

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