Tailoring hybrid glyco-nanolayers composed of chitohexaose and cellohexaose for cell culture applications

Yoshiike, Yuka; Kitaoka, Takuya

doi:10.1039/c1jm11448d

Cited by 15 publications

(20 citation statements)

References 33 publications

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“…It is possible to immobilize or form microarrays of a wide variety of carbohydrates on gold surfaces using SAM techniques. Different types of monosaccharides [17,21,27,[35][36][37][38][39][40][41][42][43][44][45][46], disaccharides [42,43,[47][48][49][50], oligosaccharides [45,46,[49][50][51][52][53][54], polysaccharides [14], and dendrimers [4,5,26] can be immobilized as a terminal functional group of the SAM depending on the nature of the study. Table 2 lists some of the carbohydrates used as terminal functional groups of the SAM and their applications.…”

Section: Types Of Terminal Carbohydratesmentioning

confidence: 99%

“…Oligosaccharides Chitohexaose -for immobilization of cells and use the surface for cell culture applications [53,54] β-Cyclodextrin -to enhance the loading capacity of anticancer drug methotrexate on gold nanoparticles [45] Dendrimers Generation 2 dendrimer with terminal carbohydrates (Figure 4) Generation 3 dendrimer with terminal mannose group -to determine association constants and kinetic rate constants with corresponding specific lectins [4,5]-to enhance bacteria sensor [26] units of a methylene group (-CH 2 ), ethylene glycol (-OCH 2 CH 2 ) and the combination of both [29]. However, linkers can also be made from aromatic compounds [55], dendrimers [4,5], and peptides [56].…”

Section: Sacc Terminal Carbohydrates Applicationsmentioning

confidence: 99%

“…Even though the longer chain linkers are preferred due to various advantages, monolayers of oligosaccharides (e.g., hyaluronan, chitohexaose, and chitosan hexamer) have been successfully immobilized on gold by modifying the reducing ends of the carbohydrates with thiosemicarbazide (TSC) ( Figure 6) [53,58,59]. The immobilized oligosaccharides SAMs were then used for specifically capturing different types of cell to be used in cell culture applications [53,59,60].…”

Section: Sacc Terminal Carbohydrates Applicationsmentioning

confidence: 99%

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Self-Assembled Monolayers of Carbohydrate Derivatives on Gold Surfaces

Bhattarai¹,

Neupane²,

Mikhaylov³

et al. 2017

Carbohydrate

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Self-assembled monolayers (SAMs) presenting carbohydrates (glycans) have been widely prepared on gold surfaces to mimic the carbohydrate surfaces that are involved in molecular recognition phenomena in living cells. The binding affinity of carbohydrate immbolized on SAM surfaces to various carbohydrate-binding proteins (such as lectins) can be studied by optical, electrochemical, piezoelectrical and thermal sensing techniques. The lectins present on the surface of pathogens (e.g., bacteria or viruses) can be used as targets for capturing onto carbohydrates immobilized on SAM surfaces. The immobilized carbohydrates can also be used for detecting different types of disease biomarkers present in bodily fluids. Synergistic properties of carbohydrate SAMs and gold nanoparticles can be used for vaccine preparation and drug delivery. By studying different types of glycans, their properties, and the behavior toward recognition of specific pathogens and biomarkers, we can develop not only new therapeutics but also enhance the diagnostic strategies of various diseases. In this chapter, we discuss carbohydrate-terminated SAMs and their common preparation strategies. Next, we focus on roles of different components of SAMs, characterization techniques, and applications.

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Section: Types Of Terminal Carbohydratesmentioning

confidence: 99%

Section: Sacc Terminal Carbohydrates Applicationsmentioning

confidence: 99%

Section: Sacc Terminal Carbohydrates Applicationsmentioning

confidence: 99%

See 1 more Smart Citation

Self-Assembled Monolayers of Carbohydrate Derivatives on Gold Surfaces

Bhattarai¹,

Neupane²,

Mikhaylov³

et al. 2017

Carbohydrate

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show abstract

“…In this study, we present a new approach, fabricating cell culture scaffolds that promote biological cellular organization via bioactive oligosaccharides. We previously reported various artificially-designed glyco-biointerfaces, such as self-assembled monolayers (SAMs) of chitin/chitosan, hyaluronan, cellulose and its derivatives, which were formed on gold (Au) surfaces [ 23 , 25 , 26 , 27 ]. The reducing end groups of carbohydrate polymers/oligomers were functionalized by a chemoselective modification with thiosemicarbazide (TSC), which enabled S–Au chemisorption to form the SAMs.…”

Section: Introductionmentioning

confidence: 99%

“…The reducing end groups of carbohydrate polymers/oligomers were functionalized by a chemoselective modification with thiosemicarbazide (TSC), which enabled S–Au chemisorption to form the SAMs. The glyco-chains of the SAMs were vertically aligned on the Au surfaces when chitohexaose and cellohexaose were used [ 27 ]. This facile approach provided exposed sugar ligands (nonreducing end groups) on the substrate surface.…”

Section: Introductionmentioning

confidence: 99%

Chitooligomer-Immobilized Biointerfaces with Micropatterned Geometries for Unidirectional Alignment of Myoblast Cells

Poosala

Kitaoka

2016

Biomolecules

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Skeletal muscle possesses a robust capacity to regenerate functional architectures with a unidirectional orientation. In this study, we successfully arranged skeletal myoblast (C2C12) cells along micropatterned gold strips on which chitohexaose was deposited via a vectorial chain immobilization approach. Hexa-N-acetyl-d-glucosamine (GlcNAc6) was site-selectively modified at its reducing end with thiosemicarbazide, then immobilized on a gold substrate in striped micropatterns via S–Au chemisorption. Gold micropatterns ranged from 100 to 1000 µm in width. Effects of patterning geometries on C2C12 cell alignment, morphology, and gene expression were investigated. Unidirectional alignment of C2C12 cells having GlcNAc6 receptors was clearly observed along the micropatterns. Decreasing striped pattern width increased cell attachment and proliferation, suggesting that the fixed GlcNAc6 and micropatterns impacted cell function. Possibly, interactions between nonreducing end groups of fixed GlcNAc6 and cell surface receptors initiated cellular alignment. Our technique for mimicking native tissue organization should advance applications in tissue engineering.

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Bioadaptive Porous 3D Scaffolds Comprising Cellulose and Chitosan Nanofibers Constructed by Pickering Emulsion Templating

Hatakeyama

Kitaoka

2022

Adv Funct Materials

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Highly porous three‐dimensional (3D) scaffolds can mimic the lobular structure of a human liver where hepatocytes are organized. However, 3D scaffolds with uniformly porous and oriented structures are challenging to fabricate without cross‐linking agents. Herein, this work presents a Pickering emulsion‐induced interface approach to construct aligned porous scaffolds for 3D cell cultures through the combined use of surface‐carboxylated cellulose nanofibers and chitosan nanofibers as stabilizers, and freezing/lyophilization to remove the oil phase. The obtained Pickering emulsions exhibit long‐term stability and their droplet sizes are tunable from 2.7 to 10.2 µm. Assembly at the oil–water interface can be modulated by controlling the NaCl dosage and oil phase proportion, resulting in porous foams with tunable porosity and versatile architectures as an in vitro alternative to the native liver microenvironment. The foams are noncytotoxic, confirmed using mouse fibroblast NIH/3T3 cells, and the cells grow both on the surface and in the internal structure of the foam. Notably, the 3D porous scaffolds are favorable microenvironments for the formation of human liver carcinoma HepG2 spheroidal cells, which exhibit liver‐like activity. This strategy based on Pickering emulsion templating provides a new avenue for constructing bioadaptive 3D scaffolds, specifically all‐biomass porous foams, for tissue engineering.

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Tailoring hybrid glyco-nanolayers composed of chitohexaose and cellohexaose for cell culture applications

Cited by 15 publications

References 33 publications

Self-Assembled Monolayers of Carbohydrate Derivatives on Gold Surfaces

Self-Assembled Monolayers of Carbohydrate Derivatives on Gold Surfaces

Chitooligomer-Immobilized Biointerfaces with Micropatterned Geometries for Unidirectional Alignment of Myoblast Cells

Bioadaptive Porous 3D Scaffolds Comprising Cellulose and Chitosan Nanofibers Constructed by Pickering Emulsion Templating

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