Tailoring gas sensor arrays via the design of short peptides sequences as binding elements

Mascini, Marcello; Pizzoni, Daniel; Pérez, Germán; Chiarappa, Emilio; Natale, Corrado Di; Pittia, Paola; Compagnone, Darío

doi:10.1016/j.bios.2016.09.028

Cited by 38 publications

(41 citation statements)

References 25 publications

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“…From the results obtained was clear that larger structures gave better results in terms of chemical classes detection and discrimination. However, if compared with peptides from a previous work (Mascini et al, 2017) detection specificity of oligonucleotides appeared limited. As a consequence, oligonucleotides proposed in this work can be only used for the classification of different VOCs patterns rather than to track individual VOC.…”

Section: Ccagmentioning

confidence: 81%

“…In-silico rationally designed molecular traps have been demonstrated to have a strong impact on the development of analytical strategies since they minimize experimental issues such as reagent stability and nonspecific recognition also for separation procedures (Baggiani et al, 2013;Mascini et al, 2013;Narcisi et al, 2011;Uzun and Turner, 2016). A computational approach was also recently used to reduce the large number of attempts necessary to select the right combination of tools for different gas sensing application (Gustafson and Wilmer, 2017;Mascini et al, 2017;Pizzoni et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Hairpin DNA-AuNPs as molecular binding elements for the detection of volatile organic compounds

Mascini

Gaggiotti

Pelle

et al. 2019

Biosensors and Bioelectronics

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Hairpin DNA (hpDNA) loops were used for the first time as molecular binding elements in gas analysis. The hpDNA loops sequences of unpaired bases were studied in-silico to evaluate the binding versus four chemical classes (alcohols, aldehydes, esters and ketones) of volatile organic compounds (VOCs). The virtual binding score trend was correlated to the oligonucleotide size and increased of about 25% from tetramer to hexamer. Two tetramer and pentamer and three hexamer loops were selected to test the recognition ability of the DNA motif. The selection was carried out trying to maximize differences among chemical classes in order to evaluate the ability of the sensors to work as an array. All oligonucleotides showed similar trends with best binding scores for alcohols followed by esters, aldehydes and ketones. The seven ssDNA loops (CCAG, TTCT, CCCGA, TAAGT, ATAATC, CATGTC and CTGCAA) were then extended with the same double helix stem of four base pair DNA (GAAG to 5' end and CTTC to 3' end) and covalently bound to gold nanoparticles (AuNPs) using a thiol spacer attached to 5' end of the hpDNA. HpDNA-AuNPs were deposited onto 20 MHz quartz crystal microbalances (QCMs) to form the gas piezoelectric sensors. An estimation of relative binding affinities was obtained using different amounts of eight VOCs (ethanol, 3-methylbutan-1-ol, 1-pentanol, octanal, nonanal, ethyl acetate, ethyl octanoate, and butane-2,3-dione) representative of the four chemical classes. In agreement with the predicted simulation, hexamer DNA loops improved by two orders of magnitude the binding affinity highlighting the key role of the hpDNA loop size. Using the sensors as an array a clear discrimination of VOCs on the basis of molecular weight and functional groups was achieved, analyzing the experimental with principal components analysis (PCA) demonstrating that HpDNA is a promising molecular binding element for analysis of VOCs.

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Section: Ccagmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Hairpin DNA-AuNPs as molecular binding elements for the detection of volatile organic compounds

Mascini

Gaggiotti

Pelle

et al. 2019

Biosensors and Bioelectronics

Self Cite

View full text Add to dashboard Cite

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“…It was advantageous to introduce the peptides as peptide-functionalized AuNPs instead of immobilizing monolayers of peptides as the use of the AuNPs increased the sensitivity by two orders of magnitude. Aside from VOC discrimination and detecting food aromas in different solvents, arrays with peptide-functionalized AuNPs differentiated satisfactorily between extra virgin and virgin olive oils [30,145,146]. Using ZnO nanoparticles instead of AuNPs led to similar results regarding the discrimination ability between five alcohols and three esters.…”

Section: Qcm Multi-sensor Arraysmentioning

confidence: 83%

Bulk and Surface Acoustic Wave Sensor Arrays for Multi-Analyte Detection: A Review

Länge

2019

Sensors

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Bulk acoustic wave (BAW) and surface acoustic wave (SAW) sensor devices have successfully been used in a wide variety of gas sensing, liquid sensing, and biosensing applications. Devices include BAW sensors using thickness shear modes and SAW sensors using Rayleigh waves or horizontally polarized shear waves (HPSWs). Analyte specificity and selectivity of the sensors are determined by the sensor coatings. If a group of analytes is to be detected or if only selective coatings (i.e., coatings responding to more than one analyte) are available, the use of multi-sensor arrays is advantageous, as the evaluation of the resulting signal patterns allows qualitative and quantitative characterization of the sample. Virtual sensor arrays utilize only one sensor but combine it with enhanced signal evaluation methods or preceding sample separation, which results in similar results as obtained with multi-sensor arrays. Both array types have shown to be promising with regard to system integration and low costs. This review discusses principles and design considerations for acoustic multi-sensor and virtual sensor arrays and outlines the use of these arrays in multi-analyte detection applications, focusing mainly on developments of the past decade.

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“…They demonstrated readiness of the concept for construction of real gas detector. Volatile chemical compounds including food aromas from classes of alcohols, aldehydes, esters, hydrocarbons and ketones appear to be detectable by this type of biosensor [ 65 , 66 ]. Peptide receptors were also investigated as a platform for the detection of glucose [ 67 ].…”

Section: Other Types Of Piezoelectric Biosensorsmentioning

confidence: 99%

Overview of Piezoelectric Biosensors, Immunosensors and DNA Sensors and Their Applications

2018

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Piezoelectric biosensors are a group of analytical devices working on a principle of affinity interaction recording. A piezoelectric platform or piezoelectric crystal is a sensor part working on the principle of oscillations change due to a mass bound on the piezoelectric crystal surface. In this review, biosensors having their surface modified with an antibody or antigen, with a molecularly imprinted polymer, with genetic information like single stranded DNA, and biosensors with bound receptors of organic of biochemical origin, are presented and discussed. The mentioned recognition parts are frequently combined with use of nanoparticles and applications in this way are also introduced. An overview of the current literature is given and the methods presented are commented upon.

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Tailoring gas sensor arrays via the design of short peptides sequences as binding elements

Cited by 38 publications

References 25 publications

Hairpin DNA-AuNPs as molecular binding elements for the detection of volatile organic compounds

Hairpin DNA-AuNPs as molecular binding elements for the detection of volatile organic compounds

Bulk and Surface Acoustic Wave Sensor Arrays for Multi-Analyte Detection: A Review

Overview of Piezoelectric Biosensors, Immunosensors and DNA Sensors and Their Applications

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