Tahibacter caeni sp. nov., isolated from activated sludge

Abstract

A Gram-reaction-negative, aerobic, rod-shaped, non-spore-forming, non-motile bacterial strain, designated BUT-6 T , was isolated from activated sludge of a wastewater-treatment facility. The strain grew at 15-35 6C (optimum 30 6C), pH 4.0-10.0 (optimum pH 7.0) and 0-3.0 % (w/v) NaCl (optimum 1.0 %). Phylogenetic analysis based on 16S rRNA sequences showed that strain T was most closely related to Tahibacter aquaticus PYM5-11 T (98.6 % similarity). However, the DNA-DNA relatedness between strain BUT-6 T and T. aquaticus PYM5-11 T was 47.1 %. The major fatty acids (.10 % of total fatty acids) of strain BUT-6 T were iso-C 15 : 0 , iso-C 17 : 1 v9c and iso-C 17 : 0 . The major respiratory quinone was ubiquinone Q-8. The profile of polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, an unidentified aminophospholipid, three unknown aminolipids and unidentified phospholipids. The DNA G+C content of strain BUT-6 T was 71.7 mol%. On the basis of the data from the polyphasic taxonomic study presented, strain BUT-6

Tahibacter soli sp. nov., isolated from soil and Tahibacter amnicola sp. nov., isolated from freshwater

“…The genome sizes of strains BL T and W38 T were 6 654 kb and 6 459 kb, respectively, and the genome of strain BL T contained 5 485 total genes, 5 420 protein-coding sequences, nine rRNA genes (three rRNA operons), and 52 tRNA genes for 20 amino acid synthesis, whereas the genome of strain W38 T contained 5 131 total genes, 4 992 protein coding sequences, six rRNA genes (two rRNA operons), and 54 tRNA genes for 20 amino acid synthesis, respectively. The genomic DNA G+C contents of strains BL T and W38 T were 68.3 and 65.3 %, respectively, which were within the range of the G+C contents of Tahibacter species [1, 2]. The general genomic features between strains BL T and W38 T and Tahibacter species are summarized and compared in .…”

“…At the time of writing, the genus includes only two validly published species, Tahibacter aquaticus and Tahibacter caeni , which were isolated from a drinking water supply system and activated sludge, respectively [1, 2]. Members of the genus Tahibacter are generally aerobic, Gram-stain-negative, catalase- and oxidase-positive, and non-motile rod bacteria containing ubiquinone-8 (Q8) as the major respiratory quinone, iso-C 15 : 0 and iso-C 17 : 1 ω 9 c as major cellular fatty acids, and diphosphatidylglycerol (DPG) and phosphatidylmonomethylethanolamine (PME) as the major polar lipids [1, 2]. In this study, two putative novel strains belonging to the genus Tahibacter were isolated from soil and freshwater samples collected in the Republic of Korea and taxonomically characterized using a polyphasic approach.…”

Tahibacter harae sp. nov., isolated from pig farm soil in Guangdong, PR China

“… ‡Data from Wu et al . [3]. §AL, unknown aminolipid; APL, unknown aminophospholipid; DPG, diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PL, unknown phospholipid; PME, phosphatidylmonomethylethanolamine; L, unknown lipids. …”

“…The genus Tahibacter was recently moved to the newly established family Rhodanobacteraceae ( Lysobacterales , Gammaproteobacteria ) [1]. Members of the genus Tahibacter are aerobic, Gram-reaction-negative, non-spore-forming, non-motile and rod-shaped, having Q-8 as the major respiratory lipoquinone [2, 3]. Since Makk et al .…”

“…isolated three bacterial strains from the drinking water supply system of Budapest (Hungary) and then created the genus Tahibacter with Tahibacter aquaticus PYM5-11 T as the type species in 2010 [2], it has experienced a slowly growing membership. In 2015, Tahibacter caeni BUT-6 T was isolated from the activated sludge of a wastewater-treatment facility in Kunshan, Jiangsu Province, PR China [3]. Until the time of writing this article, phylotypes affiliated with the genus Tahibacter have not been isolated in the natural environments, only these two species with valid published names.…”