Systems metabolic engineering of Escherichia coli for production of the antitumor drugs violacein and deoxyviolacein

Rodrigues, André; Trachtmann, Nathalie; Becker, Jörg; Lohanatha, Ananta F.; Blotenberg, Jana; Bolten, Christoph J.; Korneli, Claudia; Lima, André Oliveira de Souza; Porto, Luismar Marques; Sprenger, Georg A.; Wittmann, Christoph

doi:10.1016/j.ymben.2013.08.004

Cited by 115 publications

(125 citation statements)

References 57 publications

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“…The nature of the polymerization can be divided into four categories. First, the dimer diacetopiperazepine structure has two polysulfide bridge structures (12-14, 18-21, 24-26); the second, dimer diacetopiperazine piperazine structure only one polysulfide bridge structure (22)(23)(27)(28)(29)(30); third, dimer diacetopiperazine piperazine structure has no poly polysulfide bridge structure (31-35); fourth, dimer diacetopiperazine piperazine structure has only one diketopiperazine structure and one indole structure polymerization 23 (15)(16)(17). Leptosin compounds showed different cytotoxic activity against P388 tumor cell lines.…”

Section: Biosynthetic Pathways Of Isoindole From Acetyl Coamentioning

confidence: 99%

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Qian¹,

Guo²,

Dong³

et al. 2017

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A lot ofindole compounds possessing complex structures exist widely in alkaloids with enormous research significance. Specifically, bisindoles play a great role in the treatment of cancer, tumors, cardiovascular and cerebrovascular diseases. Many researchers are devoted to their biosynthesis. The structural diversity of bisindoles is attributed to multiple active sites of tryptophan. They presented bioactivities against anti-inflammatory, anti-bacterial, anti-virus and anti-cancer properties at the C-3 site of bisindoles. This paper reviews recent advances on biosynthesis pathways and bioactivites of bisindoles.

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Section: Biosynthetic Pathways Of Isoindole From Acetyl Coamentioning

confidence: 99%

“…[16] A variety of bisindole compounds, such as Coscinamide, T-opsentin, and Nortaxentin, have been found in the metabolites of deep sea sponges and exhited anti-inflammatory, antibacterial, antivirus, antibioactivity. [17][18][19][20] A series of bis(indole) glyoxamides have been designed and synthesized.…”

Section: Bioactivities Of Indolesmentioning

confidence: 99%

Qian¹,

Guo²,

Dong³

et al. 2017

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“…Additional modifications applied in L-TRP overproducers include the overexpression of exporter protein YddG [61,78,79], the inactivation of permeases AroP, Mtr and TnaB to avoid re-internalization [61,79,80], the deletion of gene tnaA coding for a tryptophanase to avoid product degradation [59,81,82] and expression of genes included in the tryptophan biosynthetic branch, including a feedback-resistant version of anthranilate synthase, TrpE fbr [60,81]. Variations on the strategies described in this section have also been applied to the production of other valuable compounds derived from the AAA pathway such as phenyllactate, phenylacetate and phenylethanol [83], L-DOPA [84], mandelic acid [85], deoxyviolacein and violacein [82,86], avenanthramides [87], and resveratrol [88] (Table 2).…”

Section: Introductionmentioning

confidence: 99%

“…Variations on the strategies described in this section have also been applied to the production of other valuable compounds derived from the AAA pathway such as phenyllactate, phenylacetate and phenylethanol [83], L-DOPA [84], mandelic acid [85], deoxyviolacein and violacein [82,86], avenanthramides [87], and resveratrol [88] (Table 2).…”

Section: Introductionmentioning

confidence: 99%

Engineering

et al. 2014

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The production of aromatic amino acids using fermentation processes with recombinant microorganisms can be an advantageous approach to reach their global demands. In addition, a large array of compounds with alimentary and pharmaceutical applications can potentially be synthesized from intermediates of this metabolic pathway. However, contrary to other amino acids and primary metabolites, the artificial channelling of building blocks from central metabolism towards the aromatic amino acid pathway is complicated to achieve in an efficient manner. The length and complex regulation of this pathway have progressively called for the employment of more integral approaches, promoting the merge of complementary tools and techniques in order to surpass metabolic and regulatory bottlenecks. As a result, relevant insights on the subject have been obtained during the last years, especially with genetically modified strains of Escherichia coli. By combining metabolic engineering strategies with developments in synthetic biology, systems biology and bioprocess engineering, notable advances were achieved regarding the generation, characterization and optimization of E. coli strains for the overproduction of aromatic amino acids, some of their precursors and related compounds. In this paper we review and compare recent successful reports dealing with the modification of metabolic traits to attain these objectives.

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“…However, inducible protein expression in L. lactis is relatively complex and there are no available light‐induced gene expression strategies in this system. Being the workhorse of genetic engineering, innumerable systems have successfully engineered Escherichia coli (E. coli) for bacterial ligand display,14, 15, 16 secretory protein expression,17, 18, 19 metabolic engineering,20, 21, 22 non‐natural amino acid incorporation,23 etc. Optogenetic strategies to regulate these processes by light are also available 24, 25, 26, 27.…”

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confidence: 99%

Toward Light‐Regulated Living Biomaterials

et al. 2018

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Living materials are an emergent material class, infused with the productive, adaptive, and regenerative properties of living organisms. Property regulation in living materials requires encoding responsive units in the living components to allow external manipulation of their function. Here, an optoregulated Escherichia coli (E. coli)‐based living biomaterial that can be externally addressed using light to interact with mammalian cells is demonstrated. This is achieved by using a photoactivatable inducer of gene expression and bacterial surface display technology to present an integrin‐specific miniprotein on the outer membrane of an endotoxin‐free E. coli strain. Hydrogel surfaces functionalized with the bacteria can expose cell adhesive molecules upon in situ light‐activation, and trigger cell adhesion. Surface immobilized bacteria are able to deliver a fluorescent protein to the mammalian cells with which they are interacting, indicating the potential of such a bacterial material to deliver molecules to cells in a targeted manner.

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Systems metabolic engineering of Escherichia coli for production of the antitumor drugs violacein and deoxyviolacein

Cited by 115 publications

References 57 publications

Engineering

Toward Light‐Regulated Living Biomaterials

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Systems metabolic engineering of Escherichia coli for production of the antitumor drugs violacein and deoxyviolacein

Cited by 115 publications

References 57 publications

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Toward Light‐Regulated Living Biomaterials

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