Systemic murine cathelicidin CRAMP safely attenuated colonic neutrophil infiltration in pigs

Fodor, Cristina C.; McCorkell, Robert; Muench, Gregory; Cobo, Eduardo R.

doi:10.1016/j.vetimm.2022.110443

Cited by 2 publications

(3 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to anti-biofilm activity, another study showed that CRAMP enhanced gut homeostasis as an immunomodulatory treatment for infectious colitis in pigs, and CRAMP showed no adverse side effects [ 62 ]. Although CRAMP exerts its immunomodulatory function to protect the host against microbial infection, it is still necessary to explore the efficacy of CRAMP against biofilms derived from different bacteria that cause infection in animals.…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas aeruginosa biofilm dispersion by the mouse antimicrobial peptide CRAMP

Zhang

Peng²,

Wang³

et al. 2022

Vet Res

View full text Add to dashboard Cite

Pseudomonas aeruginosa (P. aeruginosa) is a known bacterium that produces biofilms and causes severe infection. Furthermore, P. aeruginosa biofilms are extremely difficult to eradicate, leading to the development of chronic and antibiotic-resistant infections. Our previous study showed that a cathelicidin-related antimicrobial peptide (CRAMP) inhibits the formation of P. aeruginosa biofilms and markedly reduces the biomass of preformed biofilms, while the mechanism of eradicating bacterial biofilms remains elusive. Therefore, in this study, the potential mechanism by which CRAMP eradicates P. aeruginosa biofilms was investigated through an integrative analysis of transcriptomic, proteomic, and metabolomic data. The omics data revealed CRAMP functioned against P. aeruginosa biofilms by different pathways, including the Pseudomonas quinolone signal (PQS) system, cyclic dimeric guanosine monophosphate (c-di-GMP) signalling pathway, and synthesis pathways of exopolysaccharides and rhamnolipid. Moreover, a total of 2914 differential transcripts, 785 differential proteins, and 280 differential metabolites were identified. A series of phenotypic validation tests demonstrated that CRAMP reduced the c-di-GMP level with a decrease in exopolysaccharides, especially alginate, in P. aeruginosa PAO1 biofilm cells, improved bacterial flagellar motility, and increased the rhamnolipid content, contributing to the dispersion of biofilms. Our study provides new insight into the development of CRAMP as a potentially effective antibiofilm dispersant.

show abstract

Section: Discussionmentioning

confidence: 99%

Pseudomonas aeruginosa biofilm dispersion by the mouse antimicrobial peptide CRAMP

Zhang

Peng²,

Wang³

et al. 2022

Vet Res

View full text Add to dashboard Cite

show abstract

“…Mice were housed under standard laboratory conditions at constant temperature Correlation analysis among differential serum metabolites and gut microbiota. (Li et al, 2020;Fodor et al, 2022). Changes in body weight were measured daily during the experimental period.…”

Section: Animal Experimental Methodsmentioning

confidence: 99%

“…The DSS and DSS + Cramp groups were administered 3% DSS (wt/vol; MW 36–50 kDa, MP Biomedicals, Solon, OH, United States) for 7 days. The CRAMP peptide was synthesized by ChinaPeptides Co., Ltd. (Shanghai, China) and injected intraperitoneally once daily starting on day 4 of the experimental period as described previously ( Li et al, 2020 ; Fodor et al, 2022 ). Changes in body weight were measured daily during the experimental period.…”

Section: Methodsmentioning

confidence: 99%

Alterations in metabolome and microbiome: new clues on cathelicidin-related antimicrobial peptide alleviates acute ulcerative colitis

Jiang,

Liu,

Wang

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

Ulcerative colitis (UC) is a chronic and recurrent inflammatory disease of the gastrointestinal tract. This study aimed to determine the effect of cathelicidin-related antimicrobial peptide (Cramp) on dextran sulfate sodium (DSS)-induced acute experimental colitis in mice and to investigate the underlying mechanisms. Acute UC was induced in C57BL/6 mice with 3% DSS for 7 days, 4 mg/kg b.w. synthetic Cramp peptide was administrated once daily starting on day 4 of the experimental period. Mice were evaluated for body weight, colon length, colon histopathology, and inflammatory cytokines in colon tissue. Using 16 s rRNA sequencing, the composition structure of gut microbiota was characterized. Metabolomic profiling of the serum was performed. The results showed that DSS treatment significantly induced intestinal damage as reflected by disease activity index, histopathological features, and colon length, while Cramp treatment significantly prevented these trends. Meanwhile, Cramp treatment decreased the levels of inflammatory cytokines in both serum and colonic tissue on DSS-induced colitis. It was also observed that DSS damaged the integrity of the intestinal epithelial barrier, whereas Cramp also played a protective role by attenuating these deteriorated effects. Furthermore, Cramp treatment reversed the oxidative stress by increasing the antioxidant enzymes of GSH-PX and decreasing the oxidant content of MDA. Notably, compared to the DSS group, Cramp treatment significantly elevated the abundance of Verrucomicrobiota at the phylum level. Furthermore, at the genus level, Parasutterella and Mucispirllum abundance was increased significantly in response to Cramp treatment, although Roseburia and Enterorhabdus reduced remarkably. Metabolic pathway analysis of serum metabolomics showed that Cramp intervention can regulate various metabolic pathways such as α-linolenic acid, taurine and hypotaurine, sphingolipid, and arachidonic acid metabolism. The study concluded that Cramp significantly ameliorated DSS-induced colonic injury, colonic inflammation, and intestinal barrier dysfunction in mice. The underlying mechanism is closely related to the metabolic alterations derived from gut microbiota.

show abstract

Systemic murine cathelicidin CRAMP safely attenuated colonic neutrophil infiltration in pigs

Cited by 2 publications

References 21 publications

Pseudomonas aeruginosa biofilm dispersion by the mouse antimicrobial peptide CRAMP

Pseudomonas aeruginosa biofilm dispersion by the mouse antimicrobial peptide CRAMP

Alterations in metabolome and microbiome: new clues on cathelicidin-related antimicrobial peptide alleviates acute ulcerative colitis

Contact Info

Product

Resources

About