Systemic Amiloride Inhibits Experimentally Induced Neovascularization

Antiplasmin, but not amiloride, prevents synovitis and cartilage damage following hemarthrosis in hemophilic mice

“…Amiloride is a specific inhibitor of u‐PA . In our study, treatment with both oral and intra‐articular amiloride failed to alter joint diameter, blood‐induced synovitis, or cartilage damage.…”

“…Amiloride is a specific inhibitor of u-PA [16]. In our study, treatment with both oral and intra-articular amilo- Oral control Oral amiloride Fig.…”

“…Results: No effects of oral and intra-articular treatment with amiloride were found. In contrast, intra-articular treatment with antiplasmin resulted in significant (P < 0.01) reductions in both synovitis (score 1, 11.1% vs. 0%; score 2, 11.1% vs. 4.2%; score 3, 61.1% vs. 16.7%; score 4, 5.6% vs. 29.2%; score 5, 11.1% vs. 20.8%; score 6, 7.7% vs. 8.3%; score 7, 0% vs. 8.3%; and score 8, 0% vs. 12.5%) and cartilage damage (score 2, 10% vs. 8.3%; score 3, 50% vs. 12.5%; score 4, 30% vs. 33.3%; score 5, 10% vs. 33.3%; and score 6, 0% vs. 16.7%) as compared with controls. Conclusions: Intra-articular treatment with antiplasmin (but not amiloride) following joint bleeding prevented synovitis and cartilage damage in hemophilic mice.…”

Antiplasmin, but not amiloride, prevents synovitis and cartilage damage following hemarthrosis in hemophilic mice

“…Amiloride is a specific inhibitor of u‐PA . In our study, treatment with both oral and intra‐articular amiloride failed to alter joint diameter, blood‐induced synovitis, or cartilage damage.…”

“…Amiloride is a specific inhibitor of u-PA [16]. In our study, treatment with both oral and intra-articular amilo- Oral control Oral amiloride Fig.…”

“…Results: No effects of oral and intra-articular treatment with amiloride were found. In contrast, intra-articular treatment with antiplasmin resulted in significant (P < 0.01) reductions in both synovitis (score 1, 11.1% vs. 0%; score 2, 11.1% vs. 4.2%; score 3, 61.1% vs. 16.7%; score 4, 5.6% vs. 29.2%; score 5, 11.1% vs. 20.8%; score 6, 7.7% vs. 8.3%; score 7, 0% vs. 8.3%; and score 8, 0% vs. 12.5%) and cartilage damage (score 2, 10% vs. 8.3%; score 3, 50% vs. 12.5%; score 4, 30% vs. 33.3%; score 5, 10% vs. 33.3%; and score 6, 0% vs. 16.7%) as compared with controls. Conclusions: Intra-articular treatment with antiplasmin (but not amiloride) following joint bleeding prevented synovitis and cartilage damage in hemophilic mice.…”

Inhibitory effects of amiloride on the current mediated by native GABAA receptors in cultured neurons of rat inferior colliculus

“…Although amiloride was originally developed as a potassium‐sparing diuretic that acts by directly blocking epithelial sodium channels in the kidneys, which promotes the loss of sodium and water from the body without depleting potassium, it has been found to inhibit urokinase plasminogen activator (uPA) 42 . Because uPA impacts on tumour grade 43,44 and patient survival 44,45 and the genes for both uPA and its receptor are upregulated 46 in CNS gliomas, amiloride suppresses primary tumour growth, 47 metastasis 48 and angiogenesis, 49,50 perhaps through its effects on uPA.…”

Inhibitory effect of triamcinolone acetonide on corneal neovascularization

“…Corneal NV plays an important role in the pathogenesis of a number of corneal disorders. It is well known that the corneal implantation provides an experimental model for angiogenesis studies [1,9,10,12,14,22]. The specific angiogenic factors leading to corneal NV are likely to be multiple and diverse.…”

Antiplasmin, but not amiloride, prevents synovitis and cartilage damage following hemarthrosis in hemophilic mice

“…Amiloride is a specific inhibitor of u‐PA . In our study, treatment with both oral and intra‐articular amiloride failed to alter joint diameter, blood‐induced synovitis, or cartilage damage.…”

“…Amiloride is a specific inhibitor of u-PA [16]. In our study, treatment with both oral and intra-articular amilo- Oral control Oral amiloride Fig.…”

“…Results: No effects of oral and intra-articular treatment with amiloride were found. In contrast, intra-articular treatment with antiplasmin resulted in significant (P < 0.01) reductions in both synovitis (score 1, 11.1% vs. 0%; score 2, 11.1% vs. 4.2%; score 3, 61.1% vs. 16.7%; score 4, 5.6% vs. 29.2%; score 5, 11.1% vs. 20.8%; score 6, 7.7% vs. 8.3%; score 7, 0% vs. 8.3%; and score 8, 0% vs. 12.5%) and cartilage damage (score 2, 10% vs. 8.3%; score 3, 50% vs. 12.5%; score 4, 30% vs. 33.3%; score 5, 10% vs. 33.3%; and score 6, 0% vs. 16.7%) as compared with controls. Conclusions: Intra-articular treatment with antiplasmin (but not amiloride) following joint bleeding prevented synovitis and cartilage damage in hemophilic mice.…”

Inhibitory effects of amiloride on the current mediated by native GABAA receptors in cultured neurons of rat inferior colliculus

“…Although amiloride was originally developed as a potassium‐sparing diuretic that acts by directly blocking epithelial sodium channels in the kidneys, which promotes the loss of sodium and water from the body without depleting potassium, it has been found to inhibit urokinase plasminogen activator (uPA) 42 . Because uPA impacts on tumour grade 43,44 and patient survival 44,45 and the genes for both uPA and its receptor are upregulated 46 in CNS gliomas, amiloride suppresses primary tumour growth, 47 metastasis 48 and angiogenesis, 49,50 perhaps through its effects on uPA.…”

Inhibitory effect of triamcinolone acetonide on corneal neovascularization

“…Corneal NV plays an important role in the pathogenesis of a number of corneal disorders. It is well known that the corneal implantation provides an experimental model for angiogenesis studies [1,9,10,12,14,22]. The specific angiogenic factors leading to corneal NV are likely to be multiple and diverse.…”

Antiplasmin, but not amiloride, prevents synovitis and cartilage damage following hemarthrosis in hemophilic mice

“…Amiloride is a specific inhibitor of u‐PA . In our study, treatment with both oral and intra‐articular amiloride failed to alter joint diameter, blood‐induced synovitis, or cartilage damage.…”

“…Amiloride is a specific inhibitor of u-PA [16]. In our study, treatment with both oral and intra-articular amilo- Oral control Oral amiloride Fig.…”

“…Results: No effects of oral and intra-articular treatment with amiloride were found. In contrast, intra-articular treatment with antiplasmin resulted in significant (P < 0.01) reductions in both synovitis (score 1, 11.1% vs. 0%; score 2, 11.1% vs. 4.2%; score 3, 61.1% vs. 16.7%; score 4, 5.6% vs. 29.2%; score 5, 11.1% vs. 20.8%; score 6, 7.7% vs. 8.3%; score 7, 0% vs. 8.3%; and score 8, 0% vs. 12.5%) and cartilage damage (score 2, 10% vs. 8.3%; score 3, 50% vs. 12.5%; score 4, 30% vs. 33.3%; score 5, 10% vs. 33.3%; and score 6, 0% vs. 16.7%) as compared with controls. Conclusions: Intra-articular treatment with antiplasmin (but not amiloride) following joint bleeding prevented synovitis and cartilage damage in hemophilic mice.…”

Inhibitory effects of amiloride on the current mediated by native GABAA receptors in cultured neurons of rat inferior colliculus

“…Although amiloride was originally developed as a potassium‐sparing diuretic that acts by directly blocking epithelial sodium channels in the kidneys, which promotes the loss of sodium and water from the body without depleting potassium, it has been found to inhibit urokinase plasminogen activator (uPA) 42 . Because uPA impacts on tumour grade 43,44 and patient survival 44,45 and the genes for both uPA and its receptor are upregulated 46 in CNS gliomas, amiloride suppresses primary tumour growth, 47 metastasis 48 and angiogenesis, 49,50 perhaps through its effects on uPA.…”

Inhibitory effect of triamcinolone acetonide on corneal neovascularization

“…Corneal NV plays an important role in the pathogenesis of a number of corneal disorders. It is well known that the corneal implantation provides an experimental model for angiogenesis studies [1,9,10,12,14,22]. The specific angiogenic factors leading to corneal NV are likely to be multiple and diverse.…”