2018
DOI: 10.1371/journal.pone.0209269
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: Decellularization of native blood vessels is a promising technology to generate 3D biological scaffolds for vascular grafting. Blood vessel decellularization has been performed in previous studies under various experimental conditions, that complicates comparison and optimization of suitable protocols. The goal of this work was to systematically compare the decellularization and recellularization efficacy of 5 different protocols utilizing the detergents sodium dodecyl sulfate (SDS), sodium deoxycholate (SDC),… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

3
84
0
1

Year Published

2018
2018
2022
2022

Publication Types

Select...
6
1

Relationship

0
7

Authors

Journals

citations
Cited by 80 publications
(88 citation statements)
references
References 47 publications
3
84
0
1
Order By: Relevance
“…Gels derived from dECM bladder were prepared as stated earlier in 24‐well plates. HUVECs were chosen as an ideal human cell type for testing due to their extensive usage in tissue engineering applications with decellularized scaffolds to promote angiogenesis and tissue regeneration . HUVECs were seeded at a density of 40,000 cells per well and cultured for 72 h. For live‐dead staining and microscopy, cells were incubated with 2 µM Calcein AM live stain and 4 µM EthD‐1 dead stain in PBS (1×; pH 7.4) with calcium and magnesium for 45 min at 37°C.…”
Section: Methodsmentioning
confidence: 99%
“…Gels derived from dECM bladder were prepared as stated earlier in 24‐well plates. HUVECs were chosen as an ideal human cell type for testing due to their extensive usage in tissue engineering applications with decellularized scaffolds to promote angiogenesis and tissue regeneration . HUVECs were seeded at a density of 40,000 cells per well and cultured for 72 h. For live‐dead staining and microscopy, cells were incubated with 2 µM Calcein AM live stain and 4 µM EthD‐1 dead stain in PBS (1×; pH 7.4) with calcium and magnesium for 45 min at 37°C.…”
Section: Methodsmentioning
confidence: 99%
“…Hence, the issues associated with donor vessel shortage and adverse immune responses can be overcome by using decellularized scaffolds obtained from allogenic or xenogenic sources. Moreover, these tissue scaffolds should be able to resist physiological blood pressure, as they possess similar structure and mechanical properties as native vessels (Simsa et al, 2018, Lin et al, 2018. Studies showed good endothelialization and smooth muscle cell migration in the media for venous grafts, and partial recellularization of the media and adventitia for arterial grafts (Porzionato et al, 2017).…”
Section: Decellularized Matricesmentioning
confidence: 99%
“…The decellularization process involves the complete removal of cells and genetic material with the simultaneous maintenance of structural proteins (Gilpin and Yang, 2017). Therefore, decellularized grafts represent the extracellular matrix of the native blood vessels (Simsa et al, 2018). There are several methods available for decellularization, including chemical, physical, and their combinations, each offering advantages and disadvantages regarding the recreation of the blood vessels (Gilpin andYang, 2017, Xu et al, 2017).…”
Section: Page | 114mentioning
confidence: 99%
See 1 more Smart Citation
“…Quando realizada a análise macroscópica, as porções ventrais, assim como a porção dorsal sacral mostraram-se bastante translúcidas e friáveis ao manuseio, demostrando uma possível desnaturação proteica da matriz extracelular (HIRATA;SATA;SANTOS, 2004). Quanto à análise microscópica, utilizada para a visualização de células resquiciais através da coloração de hematoxilina e eosina demarcando através da hematoxilina os núcleos celulares enquanto a eosina é utilizada para demarcação de fibras de colágeno, foi observada a ausência de celularidade, resultado este comprovado pela imunofluorescência por DAPI, métodos de observação utilizado em vários trabalhos com descelularização de tecidos(SIMSA et al, 2018;ZHANG et al, 2016;LEONEL et al, 2018;SADLER et al, 2019) O método de DNAg é utilizado para quantificar o DNA resquicial presente no scaffold biológico. O material é considerado descelularizado quando o nível de DNAg encontrado no tecido é menor que 50 ng/mg de tecido (CRAPO; GILBERT; BADYLAK, 2011).…”
unclassified