System level analysis of cacao seed ripening reveals a sequential interplay of primary and secondary metabolism leading to polyphenol accumulation and preparation of stress resistance

Wang, Lei; Nägele, Thomas; Doerfler, Hannes; Fragner, Lena; Chaturvedi, Palak; Nukarinen, Ella; Bellaire, Anke; Huber, Werner; Weiszmann, Jakob; Engelmeier, Doris; Ramšak, Živa; Gruden, Kristina; Weckwerth, Wolfram

doi:10.1111/tpj.13201

Cited by 53 publications

(40 citation statements)

References 79 publications

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“…Variety and differences in growth conditions might explain these different observations. Additionally, isoforms of enzymes may play different roles at a particular developmental stage (Chaturvedi et al, 2013; Ischebeck et al, 2014; Wang et al, 2016a,b), thus displaying varying dynamics during development. For instance, Fraige et al reported three isoforms of UDPase, of which two candidates decreased in abundance after veraison whereas one increased (Fraige et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

System-Level and Granger Network Analysis of Integrated Proteomic and Metabolomic Dynamics Identifies Key Points of Grape Berry Development at the Interface of Primary and Secondary Metabolism

et al. 2017

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Grapevine is a fruit crop with worldwide economic importance. The grape berry undergoes complex biochemical changes from fruit set until ripening. This ripening process and production processes define the wine quality. Thus, a thorough understanding of berry ripening is crucial for the prediction of wine quality. For a systemic analysis of grape berry development we applied mass spectrometry based platforms to analyse the metabolome and proteome of Early Campbell at 12 stages covering major developmental phases. Primary metabolites involved in central carbon metabolism, such as sugars, organic acids and amino acids together with various bioactive secondary metabolites like flavonols, flavan-3-ols and anthocyanins were annotated and quantified. At the same time, the proteomic analysis revealed the protein dynamics of the developing grape berries. Multivariate statistical analysis of the integrated metabolomic and proteomic dataset revealed the growth trajectory and corresponding metabolites and proteins contributing most to the specific developmental process. K-means clustering analysis revealed 12 highly specific clusters of co-regulated metabolites and proteins. Granger causality network analysis allowed for the identification of time-shift correlations between metabolite-metabolite, protein- protein and protein-metabolite pairs which is especially interesting for the understanding of developmental processes. The integration of metabolite and protein dynamics with their corresponding biochemical pathways revealed an energy-linked metabolism before veraison with high abundances of amino acids and accumulation of organic acids, followed by protein and secondary metabolite synthesis. Anthocyanins were strongly accumulated after veraison whereas other flavonoids were in higher abundance at early developmental stages and decreased during the grape berry developmental processes. A comparison of the anthocyanin profile of Early Campbell to other cultivars revealed similarities to Concord grape and indicates the strong effect of genetic background on metabolic partitioning in primary and secondary metabolism.

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Section: Discussionmentioning

confidence: 99%

System-Level and Granger Network Analysis of Integrated Proteomic and Metabolomic Dynamics Identifies Key Points of Grape Berry Development at the Interface of Primary and Secondary Metabolism

et al. 2017

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“…Significant different metabolites in flavonoid biosynthetic pathway were reported in purple fig mutation compared to the green control [23]. The interaction of the primary and secondary metabolites profiles combined with proteomics elucidated the accumulation of phenolic substances and other major compounds related to flavonoids accumulation during seed development in cacao [25].Over the past decade, studies related to red-fleshed apple were mainly focused on the structural and regulatory genes which play important roles in anthocyanins biosynthesis [26][27][28][29]. Little attention was paid to the identification and classification of metabolites of red-fleshed apple.…”

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confidence: 99%

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confidence: 99%

Analysis of Antioxidant Activity and Flavonoids Metabolites in Peel and Flesh of Red-Fleshed Apple Varieties

Zhang

Xu³

et al. 2020

Molecules

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In this research, we compared the phenotypical characters, total anthocyanins content, total phenols content, and antioxidant activity of red-fleshed apple cultivars 'XJ4', 'QN-5', 'DH' and 'HX1' at three fruit developmental stages. A further flavonoids metabolites study was conducted in 'XJ4' and 'DH'. We found broader variation of total anthocyanins content in the peel of the four cultivars, which might result in larger differences of free radicals scavenging rate. The most significant difference in fruit phenotype, anthocyanins content, and DPPH scavenging rate was observed between 'XJ4' and 'DH' at mature stage. Therefore, the flavonoids metabolites of 'XJ4' and 'DH' at mature stage were compared to unveil the details of anthocyanins compounds. The unique compounds pelargonidin 3-O-β-d-glucoside and cyanidin-3-O-malonylhexoside were detected only in peel and flesh of 'XJ4' but not in 'DH', which might contribute to the purple peel and dark-red flesh color of 'XJ4'. Significantly decreased upstream metabolites in the early biosynthetic genes regulated domain were found only in 'XJ4' peel but not in the flesh. This might explain why the anthocyanins content in 'XJ4' peel was decreased largely at the mature stage. Taken together, our findings will give some insight into the metabolites study in flavonoid biosynthetic pathway of red-fleshed apple. Molecules 2020, 25, 0 2 of 22The number of hydroxyl groups on two aromatic benzene rings and the double bond positions on oxygenated heterocycle have a great impact on the sensitivity of anthocyanins to oxidation [9].As strong antioxidants, anthocyanins have a strong DPPH, ·OH, and O 2 ·radicals scavenging ability and the related studies have been intensively reported in many horticultural crops [10]. Previous study found the extract of anthocyanins from purple cabbage had strong capacity to scavenge free radicals [11]. Lyophilized pomegranate anthocyanins extract had strong ability to scavenge hydroxyl and superoxide anions [12]. Purple cauliflower had high total anthocyanins contents and scavenging rate of DPPH radical [13]. Red-fleshed apples (Malussieversii f. Neidzwetzkyana (Dieck) Langenf) are valuable resources attracting much more attention because they contain extremely high levels of anthocyanins compared to white and off-white fleshed apples [14]. The enriched anthocyanins properties of red-fleshed apple made it show stronger antioxidant activity. Our previous study indicated red-fleshed apple cultivar 'QN-5' exhibited stronger capacity for DPPH, OH, and O 2 ·radicals scavenging when compared to vitamin C [15]. A recent study found red-fleshed apples can inhibit the proliferation of human breast cancer MCF-7 and MDA-MB-231 cells through studying the antioxidant and anti-proliferation properties, compared with a traditional cultivar 'Fuji' [16]. In addition, it's reported anthocyanins extract from red-fleshed apple could eliminate ROS induced oxidative damage in porcine cells [17]. Interestly, it has been found that the antioxidant activity and phenolic conten...

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“…Untargeted metabolic fingerprinting of gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-mass spectrometry (LC-MS) data coupled with chemometrics has proven to be a robust tool for multiplex metabolomic profiling from a global perspective [13,14]. In a typical analysis, tens of thousands of metabolites can be measured by LC-MS in the anaerobic digestate of manure [15].…”

Section: Introductionmentioning

confidence: 99%

Untargeted Metabolite Profiling for Screening Bioactive Compounds in Digestate of Manure under Anaerobic Digestion

Muhmood

Czekała

et al. 2019

Water

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Untargeted metabolite profiling was performed on chicken manure (CHM), swine manure (SM), cattle manure (CM), and their respective digestate by XCMS coupled with MetaboAnalyst programs. Through global chemical profiling, the chemical characteristics of different digestates and types of manure were displayed during the anaerobic digestion (AD) process. As the feed for AD, CM had less easily-degradable organics, SM contained the least O-alkyls and anomerics of carbohydrates, and CHM exhibited relatively lower bio-stability. The derived metabolite pathways of different manure during the AD process were identified by MetaboAnalyst. Twelve, 8, and 5 metabolic pathways were affected by the AD process in CHM, SM, and CM, respectively. Furthermore, bioactive compounds of digestate were detected, such as amino acids (L-arginine, L-ornithine, L-cysteine, and L-aspartate), hormones (L-adrenaline, 19-hydroxy androstenedione, and estrone), alkaloids (tryptamine and N-methyltyramine), and vitamin B5, in different types of manure and their digestates. The combination of XCMS and MetaboAnalyst programs can be an effective strategy for metabolite profiling of manure and its anaerobic digestate under different situations.

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System level analysis of cacao seed ripening reveals a sequential interplay of primary and secondary metabolism leading to polyphenol accumulation and preparation of stress resistance

Cited by 53 publications

References 79 publications

System-Level and Granger Network Analysis of Integrated Proteomic and Metabolomic Dynamics Identifies Key Points of Grape Berry Development at the Interface of Primary and Secondary Metabolism

System-Level and Granger Network Analysis of Integrated Proteomic and Metabolomic Dynamics Identifies Key Points of Grape Berry Development at the Interface of Primary and Secondary Metabolism

Analysis of Antioxidant Activity and Flavonoids Metabolites in Peel and Flesh of Red-Fleshed Apple Varieties

Untargeted Metabolite Profiling for Screening Bioactive Compounds in Digestate of Manure under Anaerobic Digestion

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