Synthetic PEG hydrogel extracellular matrix mimics for the vascularization of engineered tissues

Papavasiliou, Georgia; Turturro, Michael V.; Christenson, Megan

doi:10.1016/j.carpath.2013.01.017

Cited by 4 publications

(2 citation statements)

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“…Regenerative medicine is based on the combination of cells and scaffolds to construct artificial tissues or organs to replace, repair, or enhance biological functions of injured patients. , For the construction of vascularized three-dimensional (3D) tissues, several bottom-up approaches have been reported recently, for example, cell sheets, − hydrogels composed of extracellular matrices (ECMs), − microfluidic channel models, − and angiogenic factors embedded scaffolds. , However, these techniques have crucial limitations because of a low biocompatibility or insufficient mechanical properties of scaffolds, which are significant factors to establish functionalized 3D tissues in the field of tissue engineering. − …”

Section: Introductionmentioning

confidence: 99%

Collagen/Cellulose Nanofiber Blend Scaffolds Prepared at Various pH Conditions

Liu

Goto

Hongo

et al. 2018

ACS Appl. Bio Mater.

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Living tissues modules consist of highly organized cells and extracellular matrices (ECMs) in a hierarchical manner. Among these ECMs, type I collagen (COL), which is the most abundant protein, is widely accepted in the tissue engineering field due to its biocompatibility. However, improvement of mechanical properties of COL scaffolds still remains a challenge. We prepared the scaffold sheets with blends of COL and 2,2,6,6-tetramethylpiperidine-1-oxil(TEMPO)-oxidized cellulose nanofiber (TOCN). TOCNs with high mechanical properties reinforced COL scaffolds. Moreover, we prepared their blends under various pH conditions and investigated their mechanical properties and biocompatibilities. Sheets prepared at a higher pH possess a better mechanical performance. From Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy measurements, it is proved that the higher mechanical properties were attributed to COL triple inter helix structure, hydrogen interaction, and electrostatic interaction with TOCN. The biocompatibilities of COL/TOCN prepared at a higher pH were increased. We successfully demonstrated COL/TOCN blend materials with a high mechanical strength and high biocompatibility for scaffold materials.

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Section: Introductionmentioning

confidence: 99%

Collagen/Cellulose Nanofiber Blend Scaffolds Prepared at Various pH Conditions

Liu

Goto

Hongo

et al. 2018

ACS Appl. Bio Mater.

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“…Poly(ethylene glycol) (PEG) is a molecule with repeating (−OCH 2 CH 2 −) units, possessing advantages of biocompatibility, high solubility in water and organic solvents, being easy to be chemically modified, and absence of immunogenic/toxic response. In tissue engineering, particularly PEGDA/DMA has been used, which can be crosslinked in the presence of UV light and photoinitiator [173] . As for LTE, PEGDA/DMA has been utilized for cell encapsulation [73,74,174] , micro-patterned system [94] , bioink [175] , in vivo application [176] and so on.…”

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confidence: 99%

Gelatin methacryloyl inverted colloidal crystal scaffolds as artificial liver platform

Shirahama¹

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Artificial organs are vital for drug development since preclinical animal testing has a limitation in human toxicity prediction, occasionally leading to severe damages. In particular the liver which is an organ that serves the functions in drug metabolism and detoxification, would play a critical role in toxicity screening when reconstructed in vitro. However, primary hepatocytes lose their functions when seeded onto plain substrates, and maintaining the functions ex vivo has been challenging. One of the cues found in liver tissue engineering to keep hepatocyte phenotype is the structural dimensionality; three-dimensional (3D) culture systems which emulate the liver microenvironment, provide enhanced cell-cell interactions and cell-material interactions compared to those in two-dimensions, resulting in prolonged of hepatocyte functions. Therefore, in-vivo-like platforms that mimic the liver microstructure have been in high demand. The overall goal of this dissertation is to create a liver-mimicking platform that can aid in maintaining hepatic functions. Besides cells, the liver is composed of extracellular matrices (ECM) with highly-ordered, porous structure. On the other hand, fabrication of such ECM-based highly-ordered scaffold has been challenging. Problems lie in high viscosity and in slow crosslinking of the aqueous protein solutions for building complex configuration. In this thesis, the material chosen for the platform is a photocrosslinkable protein, gelatin methacryloyl (GelMA). Gelatin is a hydrolyzed form of collagen, which is the main component of the liver structure. While preserving biological advantages of collagen/gelatin, the functionalized gelatin can be crosslinked in minutes in the presence of ultraviolet light and a photoinitiator. Furthermore, aqueous solutions of GelMA are much less viscous than the parent materials. However, the synthesis method has not been optimized in a systematic manner, leaving room for improvement. The first work presented in this thesis is to revamp GelMA synthesis through finding appropriate buffer systems and reaction conditions such as pH, molarity, temperature, and time.

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Control of vascular network location in millimeter-sized 3D-tissues by micrometer-sized collagen coated cells

Liu

Matsusaki

Akashi

2016

Biochemical and Biophysical Research Communications

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Engineering three-dimensional (3D) vascularized constructs remains a central challenge because capillary network structures are important for sufficient oxygen and nutrient exchange to sustain the viability of engineered constructs. However, construction of 3D-tissues at single cell level has yet to be reported. Previously, we established a collagen coating method for fabricating a micrometer-sized collagen matrix on cell surfaces to control cell distance or cell densities inside tissues. In this study, a simple fabrication method is presented for constructing vascular networks in 3D-tissues over micrometer-sized or even millimeter-sized with controlled cell densities. From the results, well vascularized 3D network structures can be observed with a fluorescence label method mixing collagen coated cells and endothelia cells, indicating that constructed ECM rich tissues have the potential for vascularization, which opens up the possibility for various applications in pharmaceutical or tissue engineering fields.

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Synthetic PEG hydrogel extracellular matrix mimics for the vascularization of engineered tissues

Cited by 4 publications

References 0 publications

Collagen/Cellulose Nanofiber Blend Scaffolds Prepared at Various pH Conditions

Collagen/Cellulose Nanofiber Blend Scaffolds Prepared at Various pH Conditions

Gelatin methacryloyl inverted colloidal crystal scaffolds as artificial liver platform

Control of vascular network location in millimeter-sized 3D-tissues by micrometer-sized collagen coated cells

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