Synthesis of improved long-chain isomaltooligosaccharide, using a novel glucosyltransferase derived from Thermoanaerobacter thermocopriae, with maltodextrin

Park, Ji Yeong; Lee, So Hee; Hong, Seong-Jin; Jeong, Ji Hye; Choi, Jung‐Ho; Park, Shin-Yong; Park, Chan Soon; Lee, Ha-Nul; Kim, Young‐Min

doi:10.1016/j.enzmictec.2021.109788

Cited by 12 publications

(7 citation statements)

References 37 publications

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“…Isomaltose is a bifidobacteria proliferation factor. However, other harmful intestinal bacteria cannot utilize isomaltose, thus inhibiting the growth of harmful bacteria and promoting the adjustment of intestinal microecology to a virtuous cycle [34]. On adding 0-8 mg/mL of algae polysaccharide into the plate of L. rhamnosus culture, Zhu Yanli et al [35] found that the colony count of these bacteria increased from 3 × 10 9 CFU/mL to 5 × 10 9 CFU/mL.…”

Section: Discussionmentioning

confidence: 99%

Cloning of Cold-Adapted Dextranase and Preparation of High Degree Polymerization Isomaltooligosaccharide

Wang¹,

Lin²,

Dong³

et al. 2022

Catalysts

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Intestinal diseases are mainly caused by a decrease in the relative abundance of probiotics and an increase in the number of pathogenic bacteria due to dysbiosis of the intestinal flora. High degree polymerization isomaltooligosaccharide (IMO) can promote probiotic metabolism and proliferation. In this study, the dextranase (PsDex1711) gene of marine bacterial Pseudarthrobacter sp. RN22 was cloned and expressed in Escherichia coli BL21 (DE3). The optimal pH and temperature of the dextranase were 6.0 and 30 °C, respectively, showing the highest stability at 20 °C. The dextran T70 could be hydrolyzed to produce IMO3, IMO4, IMO5, and IMO6 with a high degree of polymerization. The hydrolysate of 1 mg/mL could significantly promote the growth of Lactobacillus and Bifidobacterium after 12 h culture and the formation of biofilms by 58.2%. The hydrolysates could promote the proliferation of probiotics. Furthermore, the IC50 of scavenging rate of DPPH, hydroxyl radical, and superoxide anion was less than 20 mg/mL. This study provides a crucial theoretical basis for the application of dextranase such as pharmaceutical and food industries.

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Section: Discussionmentioning

confidence: 99%

Cloning of Cold-Adapted Dextranase and Preparation of High Degree Polymerization Isomaltooligosaccharide

Wang¹,

Lin²,

Dong³

et al. 2022

Catalysts

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“…B. subtilis strain AP-1 was isolated and identified as previously described [ 30 ]. The 16s rRNA sequence was deposited in GenBank with accession number MW537819.1.…”

Section: Methodsmentioning

confidence: 99%

“…Long-chain IMOs have been reported to be more effective than short-chain IMOs in promoting the growth of probiotics and enhancing health benefits [ 27 , 28 , 29 ]. Accordingly, many researchers have attempted to search for a highly efficient transferase enzyme with strong transglucosylation activity in order to improve enzymatic properties by protein engineering and/or to use a synergistic enzyme cocktail for producing IMOs with high DP [ 24 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Production of a Series of Long-Chain Isomaltooligosaccharides from Maltose by Bacillus subtilis AP-1 and Associated Prebiotic Properties

Tiangpook

Nhim

Prangthip

et al. 2023

Foods

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Bacillus subtilis strain AP-1, which produces α-glucosidase with transglucosidase activity, was used to produce a series of long-chain isomaltooligosaccharides (IMOs) with degree of polymerization (DP) ranging from 2 to 14 by direct fermentation of maltose. A total IMOs yield of 36.33 g/L without contabacillusmination from glucose and maltose was achieved at 36 h of cultivation using 50 g/L of maltose, with a yield of 72.7%. IMOs were purified by size exclusion chromatography with a Superdex 30 Increase column. The molecular mass and DP of IMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). Subsequently, linkages in produced oligosaccharides were verified by enzymatic hydrolysis with α-amylase and oligo-α-1,6-glucosidase. These IMOs showed prebiotic properties, namely tolerance to acidic conditions and digestive enzymes of the gastrointestinal tract, stimulation of probiotic bacteria growth to produce short-chain fatty acids and no stimulating effect on pathogenic bacteria growth. Moreover, these IMOs were not toxic to mammalian cells at up to 5 mg/mL, indicating their biocompatibility. Therefore, this research demonstrated a simple and economical method for producing IMOs with DP2–14 without additional operations; moreover, the excellent prebiotic properties of the IMOs offer great prospects for their application in functional foods.

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“…LMW dextran with antithrombotic activity is a blood substitute in emergencies and can be used to synthesize iron dextran to treat severe anemia (Khalikova et al, 2005). As one of the promising prebiotic candidates, IMOs modulate the composition of the intestinal microbiome and mediate health-promoting benefits (Sorndech, 2018;Huang et al, 2020;Park et al, 2021). In addition, dextranase can effectively restrain and eliminate dental plaque biofilm, which is greatly significant for the inhibition and treatment of dental caries and the exploitation of the dental caries vaccine (Walker et al, 2016;Pleszczyńska et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of cold-adapted and salt-tolerant dextranase from Cellulosimicrobium sp. THN1 and its potential application for treatment of dental plaque

Zhang²,

Liu³

et al. 2022

Front. Microbiol.

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The cold-adapted and/or salt-tolerant enzymes from marine microorganisms were confirmed to be meritorious tools to enhance the efficiency of biocatalysis in industrial biotechnology. We purified and characterized a dextranase CeDex from the marine bacterium Cellulosimicrobium sp. THN1. CeDex acted in alkaline pHs (7.5–8.5) and a broad temperature range (10–50°C) with sufficient pH stability and thermostability. Remarkably, CeDex retained approximately 40% of its maximal activities at 4°C and increased its activity to 150% in 4 M NaCl, displaying prominently cold adaptation and salt tolerance. Moreover, CeDex was greatly stimulated by Mg2+, Na+, Ba2+, Ca2+ and Sr2+, and sugarcane juice always contains K+, Ca2+, Mg2+ and Na+, so CeDex will be suitable for removing dextran in the sugar industry. The main hydrolysate of CeDex was isomaltotriose, accompanied by isomaltotetraose, long-chain IOMs, and a small amount of isomaltose. The amino acid sequence of CeDex was identified from the THN1 genomic sequence by Nano LC–MS/MS and classified into the GH49 family. Notably, CeDex could prevent the formation of Streptococcus mutans biofilm and disassemble existing biofilms at 10 U/ml concentration and would have great potential to defeat biofilm-related dental caries.

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Synthesis of improved long-chain isomaltooligosaccharide, using a novel glucosyltransferase derived from Thermoanaerobacter thermocopriae, with maltodextrin

Cited by 12 publications

References 37 publications

Cloning of Cold-Adapted Dextranase and Preparation of High Degree Polymerization Isomaltooligosaccharide

Cloning of Cold-Adapted Dextranase and Preparation of High Degree Polymerization Isomaltooligosaccharide

Production of a Series of Long-Chain Isomaltooligosaccharides from Maltose by Bacillus subtilis AP-1 and Associated Prebiotic Properties

Purification and characterization of cold-adapted and salt-tolerant dextranase from Cellulosimicrobium sp. THN1 and its potential application for treatment of dental plaque

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