Synthesis of Gold Nanoparticles Using <i>Citrus macroptera</i> Fruit Extract: Anti‐Biofilm and Anticancer Activity

Majumdar, Moumita; Biswas, Suresh Chandra; Choudhury, Rupasree; Upadhyay, Priyanka; Adhikary, Arghya; Roy, Dijendra Nath; Misra, Tarun Kumar

doi:10.1002/slct.201804021

Cited by 42 publications

(14 citation statements)

References 61 publications

(92 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To understand the mechanism of antibiofilm effect of a microorganism, the estimations of these factors are important. In the presence of CM-FeNPs, these factors were thus determined following the conventional methods [31,48,51,52] and calculated percentage inhibition productions. These are depicted in Figure 7.…”

Section: Effect Of Cm-fenps On Virulence Factorsmentioning

confidence: 99%

“…[30] Here, the mediator, i. e., plant extract acts as reducing as well as stabilizing agent. Citrus species have extensively been used for synthesizing green metal NPs [31,32] because of its major component, citric acid; the salt of citric acid, i. e., sodium citrate has widely been used for synthesizing metal NPs. [33,34] In case of FeNPs synthesis, the employment of citrus plant materials are nearly unnoticed.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Green Synthesis of Iron Nanoparticles for Investigation of Biofilm Inhibition Property

et al. 2020

Self Cite

View full text Add to dashboard Cite

Green metal nanoparticles (NPs) have emerged as efficient biofilm inhibitors. Citrus macroptera (CM) fruit extract mediated iron (Fe) NPs, i. e., CM-FeNPs were thus synthesized for studying biofilm inhibition property. The shape (spherical), size (12 nm), and crystallinity (α-Fe 0 bcc) of the CM-FeNPs were confirmed by studying mainly Transmission Electron microscopy (TEM) and X-ray Diffractometer (X-RD) tools. The particles inhibit biofilm growth of a bacteria Pseudomonas aeruginosa (P. aeruginosa) by inhibiting the functions of extracellular polymeric substances (EPS) and virulence factors to ∼ 80 %. Scanning Electron microscopy (SEM) images of the bacteria in presence of the CM-FeNPs (4600 nM) in combination with azithromycin (AZI) evidence the inhibition of biofilm growth > 80 %. Molecular docking studies support experimental findings and result significant negative binding energies (e. g., isoplatydesmine/LasR: À 8.25 kcal/mole, isoplatydesmine/MvfR: À 7.56 kcal/mole) from bioactive molecules/biofilm proteins interactions. Thus, CM-FeNPs could be considered as potential biofilm inhibitors, and as an alternative to antibiotic drugs.

show abstract

Section: Effect Of Cm-fenps On Virulence Factorsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Green Synthesis of Iron Nanoparticles for Investigation of Biofilm Inhibition Property

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…The glacial acetic acid 30% (w/v) was added to dissolve the stain from adherent biofilm. The OD of each well was measured using a microplate reader (Company: Diatek; Model: LWR96) at 492 nm [28,29].…”

Section: Assessment Of Biofilm Development Under Laboratory Conditionmentioning

confidence: 99%

“…Mica sheets containing treated and untreated bacterial (P. aeruginosa) suspension were air-dried before atomic force microscopy (AFM) analysis [29,32]. The images were captured by atomic force microscopy with the help of silicon tip (Company: Bruker Multimode 8) and evaluated with compatible software (NanoScope Analysis v1.40).…”

Section: Studies Of Biofilm Topography By Atomic Force Microscopymentioning

confidence: 99%

“…A total of 2 μL cell suspension from a 48-h P. aeruginosa culture (treated and untreated) was added at the center of the plate following point inoculation methods. The plates were incubated at 37°C for 48 h [26,29]. The rapid and coordinated translocation of a bacterial population across semi-solid surfaces was checked in triplicates.…”

Section: Measurement Of Swarming Motility Of Pseudomonas Aeruginosamentioning

confidence: 99%

See 1 more Smart Citation

In vitro anti-biofilm activity of 14-deoxy-11,12-didehydroandrographolide from Andrographis paniculata against Pseudomonas aeruginosa

2019

Self Cite

View full text Add to dashboard Cite

14-Deoxy-11,12-didehydroandrographolide is a biologically active molecule present in the extract of Andrographis paniculata (Kalmegh), a classic ethnic herbal formula, which has been used for over thousand years as therapeutics to treat numerous infectious diseases like upper respiratory tract infection, urinary tract infection, and many more health issues. The present study is designed to ascertain an inhibitor against biofilm formation from the major metabolites of Andrographis paniculata, because the extract of this herb shows inhibition of bacterial quorum sensing (QS) communication and biofilm development against microorganisms. 14-Deoxy-11,12-didehydroandrographolide at 0.1 mM (sub-MIC dose) with azithromycin (6 μg/mL, sub-MIC) or gentamicin (4 μg/mL, sub-MIC) synergistically inhibits 92% biofilm production by a 48-h treatment against Pseudomonas aeruginosa. Further investigation carried out by atomic force microscopy shows promising reduction in roughness and height of biofilm in the presence of 14-deoxy-11,12-didehydroandrographolide compared with the control group. The content of extracellular polymeric substances, level of pyocyanin production, and synthesis of extracellular protease by P. aeruginosa have also been reduced significantly at around 90% in 14-deoxy-11,12-didehydroandrographolide-treated group. In conclusion, 14deoxy-11,12-didehydroandrographolide could be used as a drug molecule against biofilm development by inhibiting QS pathway in Pseudomonas aeruginosa.

show abstract

Gluconate‐Stabilized Silver Nanoparticles as pH Dependent Dual‐Nanosensor for Quantitative Evaluation of Methionine and Cysteine

et al. 2020

View full text Add to dashboard Cite

Silver nanoparticles (AgNPs) based naked‐eye and spectrophotometric dual‐nanosensor for two most important biothiols: methionine and cysteine would be highly useful for an analyst. We have explored potential use of gluconate‐stabilized AgNPs (Glu‐AgNPs) for such unique purpose. Simply, aggregation of Glu‐AgNPs by the analyte, methionine/cysteine technique was adopted. Gluconate ions make the AgNPs anionic in nature. The anionic Glu‐AgNPs, as a pH‐ dependent nanosensor are selective to sensing methionine and cysteine over other amino acids in aqueous solution. By tuning pH of the particles’ solution we have at different demonstrated that the equilibria components of methionine/cysteine at different pH may play guiding roles in aggregating the particles, consequently in selectivity and sensitivity of the nanosensor. The LOL, LOQ, and LOD for methionine are 42, 9.89 and 2.97 μM, and for cysteine are 1.4, 0.49 and 0.14 μM, respectively. The interactions of cysteine (Kasso, 3.07x105 M−1) with the anionic Glu‐AgNPs are greater than that of methionine (Kasso, 1.635x104 M−1). Overall, the recovery results from the real samples: urine, blood serum and aCsF are excellent, which may make the developed nanosensor attractive to an analyst.

show abstract

Synthesis of Gold Nanoparticles Using Citrus macroptera Fruit Extract: Anti‐Biofilm and Anticancer Activity

Cited by 42 publications

References 61 publications

Green Synthesis of Iron Nanoparticles for Investigation of Biofilm Inhibition Property

Green Synthesis of Iron Nanoparticles for Investigation of Biofilm Inhibition Property

In vitro anti-biofilm activity of 14-deoxy-11,12-didehydroandrographolide from Andrographis paniculata against Pseudomonas aeruginosa

Gluconate‐Stabilized Silver Nanoparticles as pH Dependent Dual‐Nanosensor for Quantitative Evaluation of Methionine and Cysteine

Contact Info

Product

Resources

About