Synthesis of glycosaminoglycans by chick retinal pigment epithelium in vitro

Abstract

Cultured chick embryonal retinal pigment epithelial (RPE) cells synthesized two main glycosaminoglycans (GAGs), chondroitin 4-sulfate, and heparan sulfate. Chondroitin 4-sulfate was liberated mainly in the medium (apical side) and heparan sulfate was incorporated into the cell and/or basement membrane. Glycosaminoglycans liberated into the medium were composed of chondroitin 4-sulfate (44%), keratan sulfate (22%), heparan sulfate (18%) and hyaluronate (12%). On the other hand, the GAGs in the cell and/or basement membrane were composed of heparan sulfate (52%), hyaluronate (19%), and chondroitin 4-sulfate (13%).

Interphotoreceptor Matrix Glycosaminoglycans in Bovine Eye

“…GAG, which is one of the substances ex isting abundantly in IPM, has been proven to be synthesized and secreted by both NR and RPE cells [3,6,7,12,13]. However, the exact sites of their production and metabolic course are unclear.…”

“…and found two subpopulations: one was a 'half-sulfated' chondroitin sulfate suscepti ble to testicular hyaluronidase (HAase), and the other was a 'sialoglycan' resistant to tes ticular HAase [4], Further detailed characterization about GAGs was performed successively by Bach and Berman [ 1,2], In order to explain which tissues are the productive sites of GAGs in IPM, several studies using radioactive pre cursors had been undertaken; RPE cells had been found to synthesize and secrete GAGs [3,7,8]. On the other hand, the neural retina (NR) also had been found to produce sulfated GAGs [13,14], RPE cells also synthe- sized a fucose-containing glycoprotein [9] which is presumed to be a sialoglycan.…”

Interphotoreceptor Matrix Glycosaminoglycans in Bovine Eye

“…GAG, which is one of the substances ex isting abundantly in IPM, has been proven to be synthesized and secreted by both NR and RPE cells [3,6,7,12,13]. However, the exact sites of their production and metabolic course are unclear.…”

“…and found two subpopulations: one was a 'half-sulfated' chondroitin sulfate suscepti ble to testicular hyaluronidase (HAase), and the other was a 'sialoglycan' resistant to tes ticular HAase [4], Further detailed characterization about GAGs was performed successively by Bach and Berman [ 1,2], In order to explain which tissues are the productive sites of GAGs in IPM, several studies using radioactive pre cursors had been undertaken; RPE cells had been found to synthesize and secrete GAGs [3,7,8]. On the other hand, the neural retina (NR) also had been found to produce sulfated GAGs [13,14], RPE cells also synthe- sized a fucose-containing glycoprotein [9] which is presumed to be a sialoglycan.…”

Interphotoreceptor Matrix Glycosaminoglycans in Bovine Eye

“…GAG, which is one of the substances ex isting abundantly in IPM, has been proven to be synthesized and secreted by both NR and RPE cells [3,6,7,12,13]. However, the exact sites of their production and metabolic course are unclear.…”

“…and found two subpopulations: one was a 'half-sulfated' chondroitin sulfate suscepti ble to testicular hyaluronidase (HAase), and the other was a 'sialoglycan' resistant to tes ticular HAase [4], Further detailed characterization about GAGs was performed successively by Bach and Berman [ 1,2], In order to explain which tissues are the productive sites of GAGs in IPM, several studies using radioactive pre cursors had been undertaken; RPE cells had been found to synthesize and secrete GAGs [3,7,8]. On the other hand, the neural retina (NR) also had been found to produce sulfated GAGs [13,14], RPE cells also synthe- sized a fucose-containing glycoprotein [9] which is presumed to be a sialoglycan.…”

Interphotoreceptor Matrix Glycosaminoglycans in Bovine Eye

“…GAG, which is one of the substances ex isting abundantly in IPM, has been proven to be synthesized and secreted by both NR and RPE cells [3,6,7,12,13]. However, the exact sites of their production and metabolic course are unclear.…”

“…and found two subpopulations: one was a 'half-sulfated' chondroitin sulfate suscepti ble to testicular hyaluronidase (HAase), and the other was a 'sialoglycan' resistant to tes ticular HAase [4], Further detailed characterization about GAGs was performed successively by Bach and Berman [ 1,2], In order to explain which tissues are the productive sites of GAGs in IPM, several studies using radioactive pre cursors had been undertaken; RPE cells had been found to synthesize and secrete GAGs [3,7,8]. On the other hand, the neural retina (NR) also had been found to produce sulfated GAGs [13,14], RPE cells also synthe- sized a fucose-containing glycoprotein [9] which is presumed to be a sialoglycan.…”