Synthesis of alkane hydroxylase ofPseudomonas oleovorans increases the iron requirement ofalk+ bacterial strains

Staijen, Ivo E.

doi:10.1002/(sici)1097-0290(19980120)57:2<228::aid-bit12>3.0.co;2-c

Cited by 16 publications

(2 citation statements)

References 38 publications

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“…A report on the effect of trace element requirement has shown that the iron demand in bacteria increases during the expression of alkane hydroxylase [35]. A study on the effect of iron concentration on degradation of toluene by Pseudomonas strain reports reduction in the efficiency of the culture when the iron concentration is low [3].…”

Section: Resultsmentioning

confidence: 99%

Siderophore-Producing Bacteria from a Sand Dune Ecosystem and the Effect of Sodium Benzoate on Siderophore Production by a Potential Isolate

Gaonkar

Nayak

Garg

et al. 2012

The Scientific World Journal

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Bioremediation in natural ecosystems is dependent upon the availability of micronutrients and cofactors, of which iron is one of the essential elements. Under aerobic and alkaline conditions, iron oxidizes to Fe+3creating iron deficiency. To acquire this essential growth-limiting nutrient, bacteria produce low-molecular-weight, high-affinity iron chelators termed siderophores. In this study, siderophore-producing bacteria from rhizosphere and nonrhizosphere areas of coastal sand dunes were isolated using a culture-dependent approach and were assigned to 8 different genera with the predominance ofBacillussp. Studies on the ability of these isolates to grow on sodium benzoate revealed that a pigmented bacterial culture TMR2.13 identified asPseudomonas aeruginosashowed growth on mineral salts medium (MSM) with 2% of sodium benzoate and produced a yellowish fluorescent siderophore identified as pyoverdine. This was inhibited above 54 μM of added iron in MSM with glucose without affecting growth, while, in presence of sodium benzoate, siderophore was produced even up to the presence of 108 μM of added iron. Increase in the requirement of iron for metabolism of aromatic compounds in ecosystems where the nutrient deficiencies occur naturally would be one of the regulating factors for the bioremediation process.

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Section: Resultsmentioning

confidence: 99%

Siderophore-Producing Bacteria from a Sand Dune Ecosystem and the Effect of Sodium Benzoate on Siderophore Production by a Potential Isolate

Gaonkar

Nayak

Garg

et al. 2012

The Scientific World Journal

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“…E coli and P. oleovorans ( P. putida ), when overexpressing AlkB, have been shown to have an increased cellular demand for iron. 18 Pseudomonas strains that oxidize toluene using an AlkB-like enzyme, XylM, have shown decreased toluene degradation in iron-limited continuous culture experiments. 19 Finally, iron stimulates surfactant production in Bacillus subtilis, 20 and similar mechanisms have been hypothesized for facilitating hydrocarbon oxidation in the presence of added iron in pseudomonads.…”

Section: Perspectivementioning

confidence: 99%

Perspective: what is known, and not known, about the connections between alkane oxidation and metal uptake in alkanotrophs in the marine environment

2014

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Article Summary Should iron and copper be added to the environment to stimulate the natural bioremediation of marine oil spills? The key enzymes that catalyze the oxidation of alkanes require either iron or copper, and the concentration of these ions in seawater is vanishingly low. Nevertheless, the dependence of alkane oxidation activity on external metal concentrations remains unclear. This perspective will summarize what is known about the co-regulation of alkane oxidation and metal acquisition and pose a series of critical questions to which, for the most part, we do not yet have answers. The paucity of answers points to the need for additional studies to illuminate the cellular biology connecting microbial growth on alkanes to the acquisition of metal ions.

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Production of enantiopure styrene oxide by recombinantEscherichia coli synthesizing a two-component styrene monooxygenase

Panke

Wubbolts²,

Schmid³

et al. 2000

Biotechnol. Bioeng.

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Synthesis of alkane hydroxylase ofPseudomonas oleovorans increases the iron requirement ofalk+ bacterial strains

Cited by 16 publications

References 38 publications

Siderophore-Producing Bacteria from a Sand Dune Ecosystem and the Effect of Sodium Benzoate on Siderophore Production by a Potential Isolate

Siderophore-Producing Bacteria from a Sand Dune Ecosystem and the Effect of Sodium Benzoate on Siderophore Production by a Potential Isolate

Perspective: what is known, and not known, about the connections between alkane oxidation and metal uptake in alkanotrophs in the marine environment

Production of enantiopure styrene oxide by recombinantEscherichia coli synthesizing a two-component styrene monooxygenase

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