Synthesis of a mixed-model stationary phase derived from glutamine for HPLC separation of structurally different biologically active compounds: HILIC and reversed-phase applications

Theory and Practice of UHPLC and UHPLC–MS

“…A mixed-mode glutamine silica-bonded stationary phase containing an amino alcohol group and two different amide groups, one being a polar head and the other embedded in an aromatic phenyl ring, retains polar, moderately polar, and nonpolar analytes (Aral et al, 2015). The column shows similar properties to zwitterionic stationary phases and provides a HILICeion exchange dual retention mechanism.…”

Interactions between pyrazole derived enantiomers and Chiralcel OJ: Prediction of enantiomer absolute configurations and elution order by molecular dynamics simulations

“…The column temperature also has an important effect on HPLC separation [30,31]. Therefore, in addition to the experimental temperature of 298K, calculations were also performed at high temperature (500K) and low temperature (100K) under the mobile phase 2-propanol (DC = 18.62) following the same protocol as that applied for the room temperature.…”

“…Thus it would be interesting to elucidate how solvents would affect the interactions when a polar group is introduced into the structure. On the other 5 hand, separation of chiral drugs by HPLC has been performed under different column temperature [30,31], therefore, it is also important to study if temperature has similar influence on their interactions.…”

Preparation of a monolithic column with a mixed‐mode stationary phase of reversed‐phase/hydrophilic interaction for capillary liquid chromatography

“…The stationary phases can be designed according to three different formats: (1) one ligand is functionalized with different chemical functions [1]; (2) several different ligands are immobilized onto one support; or (3) different packing material bonded with different types of ligands are mixed together in one column [6]. Thus far, the use of silica-based mixed-mode stationary phases for HPLC separations has been widely reported [7][8][9][10][11][12][13][14][15][16][17][18], while only few mixed-mode stationary phases based on the polymer monolithic columns have been reported [19,20].…”