Synthesis of 3H labeled dihydrorotenone

Synthesis, 3H‐labelling and in vitro evaluation of a substituted dipiperidine alcohol as a potential ligand for chemokine receptor 2

“…However, the multi‐step synthesis of 4 proceeds via intermediate 5 (shown in Scheme ), which was present in abundance and ready to use. Furthermore, it has been reported in the literature that under standard reaction conditions for tritio‐dehalogenations, using Pd/C and tritium gas, C═C double bonds may undergo saturation or isomerisation, even at low tritium pressures . Intermediate 5 , unlike 4 , does not contain a double bond and, therefore, did not appear at risk to undergo an unwanted reduction.…”

Tritiated dihydro compounds employed as radioligand surrogates

“…Because no details were reported for the preparation of 25 , Williams and colleagues at the former Berkeley National Tritium Labelling Facility studied its tritium labeling in greater depth . These radiochemists employed the tritiation conditions displayed in Scheme and in the course of their radiolabeling reported another single contaminant accompanying desired product 25 .…”

Synthetic Tritium Labeling: Reagents and Methodologies

“…These signals are attributed to tritium incorpoation at the 6¢ (exocyclic methine), 7¢, and 8¢ (methyl) positions. 86…”