Synthesis, Characterization, and Antibacterial Activity of Calcium Hydroxide Nanoparticles Against Gram‐Positive and Gram‐Negative Bacteria

Abstract

Calcium hydroxide nanoparticles (Ca(OH)2 NPs) are of great interest in the development of new products due to their antibacterial properties. Chemical precipitation process was used for synthesizing Ca(OH)2 NPs. The synthesized Ca(OH)2 NPs were characterized by using X‐ray Diffraction (XRD), Field Emission Scanning Electron Microscopy (FESEM), Fourier Transmission Infrared Spectroscopy (FTIR), UV‐Visible spectroscopy, DLS (Dynamic Light Scattering) techniques. Ca(OH)2 NPs were assessed for antibacterial activity against gram‐positive bacteria (Bacillus subtilis, Staphylococcus aureus) and gram‐negative bacteria (Escherichia coli, Pseudomonas aeruginosa). The results showed reasonable bactericidal activity and flash out that the antibacterial activity of Ca(OH)2 had an important inhibitory activity against B. subtilis and P. aeruginosa which are gram‐positive and gram‐negative bacteria respectively. Reactive oxygen species (ROS) generation of Ca(OH)2 NPs were also studied and shows significant results against both gram negative and gram positive bacteria. From the analysis of the results, it was observed that Ca(OH)2 shows the best antibacterial activity against gram‐positive bacteria B. subtilis. Further, the possible mechanisms of antibacterial behaviour of Ca(OH)2 against each bacteria were suggested. These outcomes indicate that Ca(OH)2 could be utilized as a functional antibacterial material.

Spectroscopic and antibacterial activities of cobalt and nickel nanoparticles: a comparative analysis

“…Cobalt nanoparticles and NiNPs have been found to possess antibacterial properties, which make them attractive for use in medical and industrial applications. Research studies have shown that these nanoparticles can effectively inhibit the growth and proliferation of bacteria of several types, encompassing Gram-positive and Gram-negative ones (Fazal et al 2022;Harish et al 2022;Sarian et al 2022;Hassan Afandy et al 2023). The antibacterial properties of these nanoparticles are thought to be due to their ability to generate reactive oxygen species (ROS) that can damage bacterial cell membranes and inhibit bacterial growth (Saravanan et al 2021).…”

Ultrasound Activatable Microbombs via Co‐Loading CaO2/Perfluorohexane for Cascade Catalytic Biofilm Disruption and Caries Prevention

“…Additionally, as a widely applied antimicrobial medicament, the highly alkaline calcium hydroxide generated from the reactions could also promote the remineralization and therapeutic recovery of tooth hard tissue. [ 37,38 ] It could be hypothesized that the proposed microbombs can be activated by US to synergistically achieve ROS‐induced bacteria inactivation and bubble‐assisted dental plaque disruption for caries prevention as well as biofilm‐related diseases treatments (Scheme 1C).…”