Synthesis and Transfection Efficiencies of Divalent Ammonium Headgroup Cationic Lipids with Different Hydrophobic Tails

Synthesis, gene transfection efficiency, and serum compatibility of β-sitosterol-based cationic lipids with different headgroups

“…In Scheme 1, β-sitosterol ( 9 ) was reacted with 4-nitrophenyl chloroformate to afford active carbonate compound 10 . 49 The achieved compound 10 was divided into 2 portions. Each portion of compound 10 was reacted with 1,2-diaminoethane and 1,3-diaminopropane to give the corresponding carbamate bond of compounds 1A and 5A , respectively.…”